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T O P I C R E V I E W
Andy Zaayenga
The Living Chip: A massively parallel microfluidics platform for ultra-high throughput screening and compound library storage
We have developed a novel microarray technology for performing very large numbers (potentially up to one million) of biochemical, chemical and cell-based nanoliter volume screening and synthesis operations in a massively parallel manner. The Living Chip is an array of precisely machined through-holes retaining nanoliters of fluid by capillary action. Sample loading, washing and recovery are operations that can be performed manually or with simple automation. Mixing between co-registered through-holes is achieved by stacking two or more precision aligned arrays and optical assay read-outs are in parallel with a CCD imaging system. An automated picker system transfers hits into lower density microtiter plates for further analysis. We will present results demonstrating massively parallel implementation of both homogeneous and inhomogeneous fluidic and cell-based assay systems and applications of the chip for drug compound library storage and management.
