FP for PKC: Conclusions
A high affinity anti-phosphoserine Ab has been discovered for development of FP assay of PKC.
Phosphatidylserine did not interfere with FP assays of PKC.
No significant propeller effect was observed.
This antibody may recognize a specific phosphopeptide only. However, the FP assay developed with the antibody may be adapted to other serine/threonine kinases since many of they share similar recognition motifs.