Orbital Shaker
Problems:
- The shaker needed for this system must be able to sufficiently agitate a small volume of solution (0.5 mL) and be able to shake up to 25 tubes at one time.
- Have the shaker stop in the same position each time that a tube was retrieved or replaced.
- Temperature control of tubes between user specified range (10oC-37.5oC).
Solutions:
- A variable speed orbital shaker (Make and model) was selected and customized by adding a 30-position rack. (See Figure 1)
- Use of pneumatic pins and guides attached to the shaker which are engaged prior to the pull. (See figure 2)
- The tubes are kept at a constant temperature while on the shaker by using a Neslab?heater/circulator and Zymark?chiller system. (See Figure 3)