Comparison Between FLISA and ELISA

(R+D systems)

Linear dynamic range IL-8: 15.6-1000 pg/ml IL-8: 31-2000 pg/ml

IL-6: 15.6-2000 pg/ml IL-6: 3.1-300 pg/ml

Amount of monoclonal antibody 0.4 ?g 38.4 ?g

required per plate

Protocol Homogeneous assay Multiple wash and incubation steps

Cost/well Beads: $0.005

Streptavidin $0.005

mAb: $0.016 mAb: $0.15

biotin-pAb: $0.018 biotin-pAb: $0.018

Total: $0.044 Total: $0.168

(excluding detection reagents)