HTS Protocol for Detecting GPCR Agonists & Antagonists

• Prepare cell plates

  • Seed Black Packard Clearview plates with ICN Multidrop
  • Cell confluency important

• Load cells with Fluo-3 AM dye

  • Probenecid for CHO-K1 cells
  • Load for 1 hour with 5 uM dye

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