Implementation
First step is to compile a list of variables and set high and low values for each, e.g.
- salt 0 150mM
- temperature 22 37C
- Ca++ 0 4mM
- enzyme conc. 5 25 nM
- glycerol 0 10%
- BSA 0 0.05%
- shaking no yes
Note that variables may be continuous or categorical and intra or interplate.