PPT Slide

Traditional Approach for Identifying Estrogen Receptor Modulators

Estrogen Receptor

Labeled

Estradiol

Compound

Secondary Assay

Characterizes compounds as

agonist or antagonist

Transcriptional Activation

of reporter genes in cell culture

Problem: Agonist or antagonist activity may depend upon cell and promoter context.

Primary Assay

Identifies compounds that compete

with labeled estradiol

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