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Mid Atlantic Chapter

February 1999
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The Laboratory Robotics Interest Group

February 1999 Meeting

Agricultural & Analytical Applications

Date:        Monday, February 22, 1999
Place:       Raritan Valley Community College Advanced Technology Communication Center
                 Route 28 & Lamington Road, North Branch, NJ  08876
Itinerary:  Social Period with Food & Refreshments and Poster Session, Lobby -  4:30 to 6:30 PM
                 Presentations and Discussion, Auditorium - 6:30 to 8:30 PM
Pre-Registration: Requested, not required.  Registering will allow us to more accurately gauge seating requirements and refreshment needs.  Indicate names of attendees and company affiliation.
                 Email:  [email protected]
                 Phone: (732)302-1038
                 Fax:     (732)302-9080

Agenda:  This is a combined session meeting focusing on Agricultural Applications and Analytical Applications.  The Social Period will feature food and refreshments.  Members interested in presenting a poster are encouraged to do so.  Open career positions at your company may be announced or posted.  There is no fee to attend the meeting.  Bring a business card to drop in the registration fishbowl - it eases registration and qualifies you for the rosewood pen set drawing.

Sharon Reed is chairing the Agricultural Session.  Bill Haller is the chair for Analytical Applications.  Their contact information is listed below.

Agricultural Session

Presentation:  Custom Modifications to Packard MultiPROBE 208 Liquid Handler and Asymtek?/font> Century 702?/font> Fluid Dispenser in Dispensing Agar-Based Insect Diet Media for Insecticide Discovery in Agricultural Biotechnology.
Tracy Michaels1, John Cesarek1, Stacey Replogle2,Mark Hurst2,Lisa Fertsch3 and Scott Breidenthal3
1Mycogen Corporation,2 Packard,3Asymtek
[email protected]

A major technical goal of our program is to reliably and consistently dispense volumes ranging from 0.1 ml to 5 ml to diverse types of insect container formats, including the standard 96-well footprint, used in insecticide screening. Dispensing of insect diet posed special challenges to the liquid dispensing process due to the elevated temperature and mixing requirements of the liquid media. Use of available laboratory liquid handing equipment with the insect diet resulted in solidification and clogging of lines and/or dispense heads. Various modifications were implemented including specialized heating, insulation, dispense timing, dispensing heads, tips, programming, and washing. These developments resulted in consistent dispensing of the liquid insect diet with the Packard MultiPROBE 208 and Asymtek?/font> Century 702?/font> . Additions to the Packard MultiPROBE 208 included an extension on the Z-axis range to enable the probes to access the liquid insect-diet reservoir positioned below the deck. The Asymtek?/font> Century 702?/font> was integrated with a Watson-Marlow Multi-Channel Peristaltic Pump for coordinated accuracy of dispensing into containers with a variety of dimensions and volumes. Each dispensing system has its own advantages and unique roles in meeting the needs of the insect bioassay system and will be discussed in detail.

Presentation:  Migration From a Traditional AgChem Screen to a Miniaturized HTS Program
Robert Bisbing, Rohm and Haas Company, Spring House, PA

Within the last two years, the strategy for and migration to a "high throughput" first level screening effort has restructured the sample handling, data acquisition, and biological testing areas within our overall evaluation program for identifying agriculturally active compounds. This change was initiated to facilitate, broaden and reduce costs associated with the sample acquisition process and to improve the quality of compounds entering our more advanced greenhouse screens. As an interim stage, traditional screens requiring large amounts of test compound were replaced with similar but modified tests requiring less compound. Concurrently, new miniaturized screens were developed and validated within each area of interest (fungicides, insecticides and herbicides). Means for converting our powder archive to liquid format was initiated along with sample handling strategy that would be consistent with new screens being developed for implementation at the workstation level. A brief description with overheads/slides of 4-5 of these tests will be given.

Presentation:  The AMPLE(TM) Multiplex Synthesis Device
Robert E. Hormann, Daryl Gilbert, Wilhelm Glaeser
Rohm and Haas Company, Spring House, PA

In the Rohm and Haas agrochemicals group, we have developed a medium-scale (75 mL x 15 vessels), semi-manual, parallel synthesis device called AMPLE(TM) (Amplified Multiplex Preparation of Library Ensembles). Once a lead structure is identified, possibly by combinatorial methods, a subset or ensemble of structures related to the active members of that library may be scaled-up, or amplified. The basic AMPLE(TM) design is flexible, compact, scalable, and adaptable to the numerous conditions of nonuniformity which are typical of traditional organic synthesis.

Poster:  FLUORESCENCE DETECTION TO MONITOR FUNGAL GROWTH IN MICROPLATE-BASED
FUNGITOXICITY ASSAYS

M. Elizabeth Miller and Christine S. Jany
Rohm and Haas Company, Spring House, PA

The fluorescent stain, FUN-1, available from Molecular Probes, was evaluated for its utility as a live/dead marker with fungi used in our current in-vitro cytotoxicity assays.

Analytical Session

Presentation:  LABORATORY AUTOMATION IN PHARMACEUTICAL DEVELOPMENT: PERSPECTIVES FOR THE NEW MILLENNIUM
Stephen Scypinski, Analytical Development
The R.W. Johnson Pharmaceutical Research Institute, Raritan, NJ

Incredibly, the use of laboratory automation and robotics in pharmaceutical analysis and drug development is almost twenty years old. There are many reported examples of success stories, trials and tribulations of pharmaceutical dosage form automation. More recently, the automation wave has hit the drug discovery area quite hard and it is amazing to see the rapid implementation of automation and robotics in areas such as high throughput screening and combinatorial chemistry. If one compares the "ramps of utilization" between discovery and development, one will find (without surprise) that discovery has far outstripped development in it’s utilization of automation. The question to be asked is…WHY?

I have asked myself the aforementioned question and have tried to answer it by factoring in the rigidities we in development are confronted with such as regulatory methods, cGMP compliance, method, system and computer validation, documentation and training. Indeed, if any or all of these factors are contributing to the non-usage of robotics in drug development and pharmaceutical analysis, their contribution is almost certainly small. One must begin to ask the question: "If we in development are indeed going to integrate robotics into our repertoire of analytical tools and techniques, what needs to be done to make this happen?"

Having joined a new organization less than a year ago helps one establish a perspective and gives a new beginning and new ideas. One approach being tried is to firmly "build automation in" when designing methods and sample preparation schemes. This accomplishes several things. 1) It shifts the paradigm of conventional sample preparation to that compatible with automation (i.e. homogenization) 2) Translating/transferring manual methods to an automated system becomes more facile and 3) Persons utilizing both see more equivalency between them and hence, usage is more readily accepted.

A tangential approach to the implementation of "classic automation" is the development of new analytical instrumentation based on high throughput technology, which holds great promise as one of the premier analytical techniques of tomorrow.

Using several examples, aspects of automation applied to the challenges we all face as we move into the 21st century will be highlighted.

For more information contact:

Executive Chair:
Dennis France
dennis.france@
pharma.novartis.com

(908) 277-5328
Novartis Pharmaceuticals Corporation
Secretary:
Andy Zaayenga
andy.zaayenga@
tekcel.com

(732) 302-1038
TekCel Corporation
Analytical Chemistry Chair and Treasurer:
William Haller
bhaller@
ompus.jnj.com

(908) 218-6341
Ortho-McNeil
High Throughput Screening Chair:
John Babiak, Ph.D.
babiakj@
war.wyeth.com

(732) 274-4788
Wyeth-Ayerst Research
Agricultural Applications Chair:
Sharon Reed
reeds@
pt.cyanamid.com

(609) 716-2905
American Cyanamid
Data Management Chair:
Steve Fillers, Ph.D.
steve_fillers@
biogen.com

(617) 679-2657
Biogen Inc.
   

Directions (on-line directions):

The Raritan Valley Community College campus lies at the crossroads of Central New Jersey, with Routes 22, 202 and 206 and Interstates 287 and 78 just minutes away. The College is situated on the north side of Route 28 in North Branch.

FROM THE NORTH

From INTERSTATE 287 SOUTH - (From Morristown Area): Take Interstate 287 SOUTH to Interstate 78 WEST. Proceed on 78 WEST for approx. 3 miles to Exit 26 (Lamington/North Branch). At light make a left onto Lamington Road. Proceed on Lamington Road til it ends (approx. 3 miles). At STOP sign make a left onto Route 28 EAST. Entrance to the College will be on your left approx. 1/8 of a mile.

From GARDEN STATE PARKWAY SOUTH - (From North Jersey): Take Exit 142 for Interstate 78 WEST. Proceed on 78 WEST for approx. 26 miles to Exit 26 (Lamington/North Branch). At light make a left onto Lamington Road. Proceed on Lamington Road til it ends (approx. 3 miles). At STOP sign make a left onto Route 28 EAST. Entrance to the College will be on your left approx. 1/8 of a mile.

FROM THE SOUTH

From INTERSTATE 287 NORTH - (From Edison Area): Take Interstate 287 NORTH to the Route 22 WEST exit. After approx. 7 miles (just before the 5th light), exit onto Orr Drive. At the end of Orr Drive make a left at the STOP sign. Make first right into the College.

From GARDEN STATE PARKWAY NORTH - (From the Shore area): Take Exit 127 to Interstate 287 NORTH. Proceed on 287 NORTH to the Route 22 WEST exit. After approx. 7 miles (just before the 5th light), exit onto Orr Drive. At the end of Orr Drive make a left at the STOP sign. Make first right into the College.

From ROUTE 206 NORTH - (From Princeton Area): Take Route 206 NORTH to the Somerville Circle. At the Somerville Circle exit onto Route 28 WEST. Proceed on Route 28 WEST for approx. 4 miles. Entrance to the College will be on your right.

FROM THE EAST

From INTERSTATE 78 WEST - (From Newark Area): Take Interstate 78 WEST. Proceed on 78 WEST and get off at Exit 26 (Lamington/North Branch). At light make a left onto Lamington Road. Take Lamington Road til it ends (approx. 3 miles). At STOP sign make a left onto Route 28 EAST. Entrance to College will be on your left approx. 1/8 of a mile.

FROM THE WEST

From INTERSTATE 78 EAST - (From Clinton Area): Take Interstate 78 EAST to Exit 26 (Lamington/North Branch). Turn right at light onto Lamington Road. Proceed on Lamington Road til it ends (approx. 3 miles). At STOP sign make a left onto Route 28 EAST. Entrance to the College will be on your left approx. 1/8 of a mile.

FROM OTHER AREAS

From NEW JERSEY TURNPIKE - Take the New Jersey Turnpike to Exit 10. After toll, bear left, exit onto Interstate 287 NORTH. Proceed on 287 NORTH to the Route 22 WEST exit. After approx. 7 miles (just before the 5th light), exit onto Orr Drive. At the end of Orr Drive make a left at the STOP sign. Make first right into the College.

From ROUTE 31 NORTH - Take Route 31 NORTH to Route 202 NORTH. Stay on Route 202 NORTH for approx. 10 miles til you come to the Somerville Circle. At the Somerville Circle exit onto Route 28 WEST. Proceed on Rt 28 WEST for approx. 4 miles. Entrance to the College will be on your right.

From PENNSYLVANIA TURNPIKE - Take either Route 78 East, or Route 22 East to Route 78 East. Follow directions above "FROM THE WEST" (From Clinton Area).

FROM THE PARKING LOT (see map of campus)

Follow signs to parking lot #2.

Area Hotels

Ramada Inn
60 Cottontail Lane & Rt. 287
Somerset, NJ  08873
732-560-9880
mention LRIG and get room for $94
Somerset Marriott Hotel
110 Davidson Avenue
Somerset, NJ  08873
800-228-9290, 732-560-0500
Best Western
1271 Rt 22 West & 287
Bridgewater, NJ
800-528-1234, 908-722-4840
Days Inn of Hillsborough
118 US Hwy 206 South
Somerville, NJ
908-685-9000
Hampton Inn
255 Davidson Avenue
Somerset, NJ  08873
908-563-1600
DoubleTree Hotel
200 Atrium Drive
Somerset, NJ  08873
732-469-2600
Somerset Hills
200 Liberty Corner Road
Warren, NJ  07059
908-647-6700
Summerfield Suites
Somerset, NJ
732-356-8000
Summerfield Suites
530 Route 22 East
Bridgewater, NJ
908-725-0800

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Last modified: October 15, 2004

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