From: "JJF (Johannes Fels)" Subject: 1536 absorbance & fluorescence reader Date: Mon, 8 Nov 1999 11:38:17 +0100 I am just curious, if somebody knows vendors selling absorbance & fluorescence readers capable to read 1536 micro titer plates. I am aware of the BMG and the Tecan readers. Regards, Johannes Fels From: "Suzanne Martinez" Subject: Re: Lab-Robotics Discussion: 1536 absorbance & fluorescencereader Date: Mon, 08 Nov 1999 08:23:29 -0800 Johannes, Unfortunately, Molecular Devices has not yet developed a 1536 platereader or fluorescence reader. However, I would like to mention that we just started to sell a 1536 platewasher(the first 1536 washer on the market), called the Embla 1536. If you are interested, please send inquiries to info@SPAMFOIL.moldev.com. Suzanne Martinez Skatron Product Manager Molecular Devices Corp. 1311 Orleans Drive Sunnyvale, CA 94089 Phone: 408-548-6305, 800-635-5577 Fax: 408-747-3602 Email: Suzanne_Martinez@SPAMFOIL.MolDev.com >>> "JJF (Johannes Fels)" 11/08/99 02:38AM >>> --------------------- Lab-Robotics Discussion --------------------- I am just curious, if somebody knows vendors selling absorbance & fluorescence readers capable to read 1536 micro titer plates. I am aware of the BMG and the Tecan readers. Regards, Johannes Fels --- Lab-Robotics Discussion To review archived messages from this list, send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just RESEND-xx (xx is a number) as the subject. You will get a single digest message for the last xx days of messages. From: "Charlie d'Estries" Subject: RE: Lab-Robotics Discussion: 1536 absorbance & fluorescence reader Date: Mon, 8 Nov 1999 11:29:33 -0800 Have you looked at LJL, LabSystems, and Wallac? Charlie d'Estries SciBiz International -----Original Message----- From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On Behalf Of JJF (Johannes Fels) Sent: Monday, November 08, 1999 2:38 AM To: Lab-Robotics Discussion Mailing List Subject: Lab-Robotics Discussion: 1536 absorbance & fluorescence reader --------------------- Lab-Robotics Discussion --------------------- I am just curious, if somebody knows vendors selling absorbance & fluorescence readers capable to read 1536 micro titer plates. I am aware of the BMG and the Tecan readers. Regards, Johannes Fels --- Lab-Robotics Discussion A digest is a single MIME-encoded message containing the contents of all the messages posted to the mailing list during the digest period. If you send the DIGEST-xx (xx is a number) command to the list, you will no longer receive individual messages. Instead you will receive digest messages at the specified interval. From: qingduo@SPAMFOIL.robot.nankai.edu.cn (Shi Qingduo) Subject: Telerobot & the use of VR in robotics Date: Wed, 10 Nov 1999 15:25:31 +0800 Hi. I would like to know the current researching hotspot directions of the telerobot technology and the use of VR in robotics. Would anybody provide some summarized information on the topics above? From: "Andy Hodge" Subject: Lab-Robotics Discussion Date: Wed, 10 Nov 1999 10:50:55 -0500 We are users of Source for Automation SDAS and ADM units within a process validation / QC environment. Are there other users that wish to directly share experiences / applications etc? Andy Hodge Section Leader QC Dev and Support Wyeth UK From: "Ashton, Mark" Subject: RE: Lab-Robotics Discussion: 96 well concentrator Date: Thu, 11 Nov 1999 08:56:32 -0000 Try the TurboVap from Zymark -----Original Message----- From: Thomas J. Baiga [mailto:tjbaiga@SPAMFOIL.charybtech.com] Sent: 25 October 1999 22:45 To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: 96 well concentrator --------------------- Lab-Robotics Discussion --------------------- At 08:36 AM 10/25/99 -0400, you wrote: >--------------------- >Lab-Robotics Discussion >--------------------- >Hello, > >I am fraction collecting peaks eluting from a chromatographic separation >into a 96 well plate. I need to concentrate these samples (i.e., evaporate >off 95% of solvent). I have seen an instrument that purges nitrogen into >the wells, however do not recall the manufacturer. > >I am interested in hearing about problems associated with product absorption >to walls allowing only qualitative data on subsequent analyses - if that is >even a problem. > >Thanks and have a great day! > >Adam Fermier >RWJ-PRI Adam, Bhi (yes, the rotovap people) just unveiled a new product called SynCore (TM) at the ILMAC Conference in Basel, Switzerland this month (Oct. 11-14). This benchtop device is a parallel evaporator which can accomodate up to four (4) microtiter plates or microtiter plate racks. It won't be available in the US until Pittcon (called your local Brinkmann rep for more info) but if you can wait a few months, it's a very nice system in terms of functionality and cost. Furthermore, this system will be able to accomodate Charybdis' new collection plates, which are anodized aluminum frame racks which adhere to the xy dimensions of the microtiter plate and hold disposable glass tubes. Current rack configurations hold 96-2ml tubes, 48-5ml tubes and 24-10ml tubes with 12-25ml tube and 6-50ml tube designs available later this month. The nice thing about collection and evaporator from glass tubes is the great rate of efficiency, better heat transfer equates to lesser chances of bumping and subsequent cross contamination, and you don't need to worry about contaminating your purified samples with extracted excipients from the polypropylene plates. By the way, the N2 blow down system your probably thinking about is the device from Zymark. Hope that helps. Tom Baiga Charybdis Technologies --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: "Curtis, Jon" Subject: RE: Lab-Robotics Discussion: 96 well concentrator Date: Thu, 11 Nov 1999 09:44:44 -0000 We have developed a 96 heated dry down station that is now available through Provair UK..... PID temp and flow controlled seems to work a treat..... http://www.porvair-sciences.com/products/prodset.htm jon curtis...... > -----Original Message----- > From: Bill Tuting [SMTP:btuting@SPAMFOIL.gilson.com] > Sent: Tuesday, October 26, 1999 3:25 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: 96 well concentrator > > --------------------- > Lab-Robotics Discussion > --------------------- > Gilson has several units that will do this. I suggest you go to their home > page, gilson.com to find out more about their products and your local > representative. > Bill Tuting > Gilson, Inc. N. NJ. > > Fermier, Adam [PRI] wrote: > > > --------------------- > > Lab-Robotics Discussion > > --------------------- > > Hello, > > > > I am fraction collecting peaks eluting from a chromatographic separation > > into a 96 well plate. I need to concentrate these samples (i.e., evaporate > > off 95% of solvent). I have seen an instrument that purges nitrogen into > > the wells, however do not recall the manufacturer. > > > > I am interested in hearing about problems associated with product absorption > > to walls allowing only qualitative data on subsequent analyses - if that is > > even a problem. > > > > Thanks and have a great day! > > > > Adam Fermier > > RWJ-PRI > > > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: gledson@SPAMFOIL.whatman.com Subject: Re: Lab-Robotics Discussion: Sonic thawing Date: Thu, 11 Nov 1999 08:31:13 -0500 I am assuming that you are referring to a 96 well, 2ml deepwell storage plate. As you may know we at Whatman Polyfiltronics produce a 384 well, 400 ul per well deepwell storage plate. This 384 well plate contains a lot more plastic than a 96 well plate. Would this different format affect the sonic thawing time? Please let me have more details. How does sonic thawing differ from microwave technology? Grahame Ledson. "Curtis, Jon" on 10/21/99 04:33:20 AM To: jpc67195@SPAMFOIL.GlaxoWellcome.co.uk cc: (bcc: Grahame Ledson/Whatman) Subject: Lab-Robotics Discussion: Sonic thawing --------------------- Lab-Robotics Discussion --------------------- If any one is interested we have been thawing deep welled microtitre plates in approx. 4 hours (< 24 hours at ambient with no degradation). I saw in one of the previous questions asked to the group queries on ultrasonic thawing. We have developed Sonic thawing and are now looking to implement it on a production system... If any one is interested drop me a line... Jon Curtis........ Jon Curtis applied technology proteomic engineering rm 3G 122 Gunnels Wood road Stevenage SG1 2NY Glaxowellcome 01438 768169 tel 01438 763563 fax --- Lab-Robotics Discussion To view statistics on this list, send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just STATS as the subject. You will receive statistics for the mailing list showing number of subscribers, domain types and messages. From: "Ashton, Mark" Subject: RE: Lab-Robotics Discussion: 96 well concentrator Date: Thu, 11 Nov 1999 16:16:52 -0000 I would recommend using the TurboVap in a hood. Also it is used with a sizeable compressor so you will need space for that. -----Original Message----- From: Anthony Zerlin [mailto:azerlin@SPAMFOIL.topmail.de] Sent: 29 October 1999 07:52 To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: 96 well concentrator --------------------- Lab-Robotics Discussion --------------------- Hello, Zymark in the US has just got a 96 well evaporator called TurboVap 96 on the market. I saw it at the ISLAR in Boston. I havn愒 tried it yet but it makes a very good impression. It is very fast, has two independant positions and doesn愒 need any hood space. Anthony Am 25.10.1999 um 14.36 Uhr schrieb "Fermier, Adam [PRI]": > --------------------- > Lab-Robotics Discussion > --------------------- > Hello, > > I am fraction collecting peaks eluting from a chromatographic separation > into a 96 well plate. I need to concentrate these samples (i.e., evaporate > off 95% of solvent). I have seen an instrument that purges nitrogen into > the wells, however do not recall the manufacturer. > > I am interested in hearing about problems associated with product absorption > to walls allowing only qualitative data on subsequent analyses - if that is > even a problem. > > Thanks and have a great day! > > Adam Fermier > RWJ-PRI > From: David Hitrys Subject: Re: Lab-Robotics Discussion: 1536 absorbance & fluorescencereader Date: Thu, 11 Nov 1999 11:56:58 -0500 Check out the new reader from Carl Zeiss. It is distributed in the U.S. by Cellomics, Inc. A lot more can be found at: http://www.cellomics.com/html/products/uhts_instrumentation.htm I know a little more about it and will be pleased to answer your questions. I can be reached at 508-877-5668. David Hitrys Suzanne Martinez wrote: > > --------------------- > Lab-Robotics Discussion > --------------------- > Johannes, > Unfortunately, Molecular Devices has not yet developed a 1536 platereader or fluorescence reader. However, I would like to mention that we just started to sell a 1536 platewasher(the first 1536 washer on the market), called the Embla 1536. If you are interested, please send inquiries to info@SPAMFOIL.moldev.com. > > Suzanne Martinez > Skatron Product Manager > Molecular Devices Corp. > 1311 Orleans Drive > Sunnyvale, CA 94089 > Phone: 408-548-6305, 800-635-5577 > Fax: 408-747-3602 > Email: Suzanne_Martinez@SPAMFOIL.MolDev.com > > >>> "JJF (Johannes Fels)" 11/08/99 02:38AM >>> > --------------------- > Lab-Robotics Discussion > --------------------- > I am just curious, if somebody knows vendors selling absorbance & > fluorescence readers capable to read 1536 micro titer plates. > I am aware of the BMG and the Tecan readers. > > Regards, > > Johannes Fels > > --- Lab-Robotics Discussion > To review archived messages from this list, send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just RESEND-xx (xx is a number) as > the subject. You will get a single digest message for the last xx > days of messages. > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: bullockb@SPAMFOIL.pt.cyanamid.com Subject: 96 well to 384 well transfer of DNA Date: Thu, 11 Nov 1999 13:20 -0400 (EDT) Hello, I have been asked by our Biotech department to test the validity of my Tecan Gensis liquid handler to transfer DNA samples from 96 well plates to 384 well plates. I have various fixed needle types as well as disposable tips. I am sure that the problems I am running into are the same that many have already experienced.These are: 1. Because of the length of the fixed tips, even a tiny deviation from being off center will cause the needle to miss the well in the 384 well plate. I can not get the needles to hit the wells continously without crashing into the plate bending the needles. 2. With the disposable tips, guaranteeing that the tips will seat themselves prefectly every time is not likely resulting in the same problem as above. 3. If disposable tip are not used how do you prevent carry over of the DNA samples from well to well since DNA loves to bind to plastics and metals? Is simple solvent washing of the needles enough to prevent carry over or is there a cocktail that can be used to pre-wash with first? I have contacted Popper & Sons to have some fixed needles made that are only an inch or two long. This may help in solving the offset of the tip but I can't be sure until I try. Any help would be much appreciative. Thanks. Billy Bullock Project Leader - Automated Synthesis American Cyanamid Co. PO Box 400 Princeton, NJ 08543 From: s Subject: Lab-Robotics Discussion: 96 well concentrator Date: Thu, 11 Nov 1999 11:25:00 -0800 Dear Adam, Why don't you use the compact unit of the N-Vap for 96 well-plates, there is the one plate evaporator. We have been doing nitrogen evaporation whether it be 24, 49, and 100 rack position for numerous years. Tom Flores Thomson Instrument Company www.hplc1.com e-mail: folks@SPAMFOIL.hplc1.com From: Andy Zaayenga Subject: Re: Lab-Robotics Discussion: 96 well to 384 well transfer ofDNA Date: Thu, 11 Nov 1999 15:49:29 -0500 Billy, We are successfully reformatting 96-well to 384-well plates with our Assay TekBench. We use our 96-channel fixed cannula pipettor. The TekBench has the ability to wash the pipettor body and needles with up to three reagents. We usually select air as a third reagent to blow out any wash residue. The wash is a true flow through wash, not a reflux. It has proven to be very effective - our clients using this method are comfortable transferring compounds with it based on their carryover studies. The wash is "passive". It is performed with the pipettor detached from the retriever arm so the arm is free to perform other tasks during the wash. Our experience pipetting with 384-well plates points up the issues you raise. The cannulas should be as short as possible while allowing you to get to the bottom of your deepest labware item. This minimizes the deflection error along the length of the tip. In our case the cannulas must be long enough to reach the bottom of a deepwell. In your case you should be able to customize a shorter length. We found that active locators were best used for aligning the plates properly. There is just too much variance in the typical robot friendly locator. We use an active cam on the locator to ensure that the position of the plate is identical every time. We also have a 384-channel fixed cannula pipettor which is even more challenging, but using these concepts has proven reliable. We are looking at a 384-channel disposable tip solution (to complement our 96-channel disposable tip pipettor). One of the difficulties with disposable tips is the plasticware. There is the seating issue you referred to as well as a straightness (symmetry) issue. The 96 tip disposables are fairly well worked out. The 384 tip disposables are not, and of course these specifications must be even tighter. Another issue for automated pipettor vendors is the tip box. It must be automation friendly, which few 384 tip boxes are. A question for you, and the automation community - how important is a 384-channel disposable tip solution to you? Thanks (hope this helps). Andy Zaayenga TekCel Corporation Mailto:andy.zaayenga@SPAMFOIL.tekcel.com Web Site: http://tekcel.com At 01:20 PM 11/11/1999 -0400, bullockb@SPAMFOIL.pt.cyanamid.com wrote to Andy: |--------------------- |Lab-Robotics Discussion |--------------------- | Hello, | | I have been asked by our Biotech department to test the validity of my | Tecan Gensis liquid handler to transfer DNA samples from 96 well | plates to 384 well plates. I have various fixed needle types as well | as disposable tips. | | I am sure that the problems I am running into are the same that many | have already experienced.These are: | | 1. Because of the length of the fixed tips, even a tiny deviation from | being off center will cause the needle to miss the well in the 384 | well plate. I can not get the needles to hit the wells continously | without crashing into the plate bending the needles. | | 2. With the disposable tips, guaranteeing that the tips will seat | themselves prefectly every time is not likely resulting in the same | problem as above. | | 3. If disposable tip are not used how do you prevent carry over of the | DNA samples from well to well since DNA loves to bind to plastics and | metals? Is simple solvent washing of the needles enough to prevent | carry over or is there a cocktail that can be used to pre-wash with | first? | | I have contacted Popper & Sons to have some fixed needles made that | are only an inch or two long. This may help in solving the offset of | the tip but I can't be sure until I try. | | Any help would be much appreciative. Thanks. | | Billy Bullock | Project Leader - Automated Synthesis | American Cyanamid Co. | PO Box 400 | Princeton, NJ 08543 | From: andy.zaayenga@SPAMFOIL.lab-robotics.org Subject: Duplicate Posts Date: Thu, 11 Nov 1999 16:25:09 -0500 Hi all, Sorry if you have received some duplicate posts. The MindSpring/Netcom servers (our ISP) have been a little flaky. When a message is interrupted mid-stream, it is re-qued, causing the already sent posts to be sent again. Andy Zaayenga Moderator, The Lab-Robotics Discussion Mailing List mailtoandy.zaayenga@SPAMFOIL.lab-robotics.org web site http//www.lab-robotics.org From: "Samir M. Dandekar" Subject: RE: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Thu, 11 Nov 1999 14:56:22 -0600 We have seen the same problem when going from 96 well PCR plates to 384 well PCR plates. There is not much "give" when you get the tips inside the wells. With the small volume tips you have about 1 mm on either side of the tip when the is placed inside a 384 plate well. We eventually got the 384 well protocols to work and what we had to do is spread the rack error over the whole plate. Since the PCR plates are plastic some error is introduced just from manufactuing along with error introduced from the carriers that these plates sit on. We implemented the racks so that starting in column 1 the tips are slightly left of center and in column 12 the tips are slightly right of center. This worked with the plates that we used, and it this approach may change from plate type to plate type. We also seat the 384 well plates in an adapter so that each well of the 384 well plate is seated in its own hole in the adapter plate. This allowed the plate to be positioned more precisely on the carrier. Going to short fixed tips is a good approach, but you will have to make your carriers taller if the tips are not long enough. As far as carryover, our customer asked us to program the protocols to perform a bleach wash in between DNA samples to prevent carryover. Our customer is running 11 different protocols from DNA/PCR pooling, gridding, and cherry picking. We used the Tecan Toolbox software to develop a custom application and implemented the racks with the Worktable Editor program I can give you our customers contact information if you would like to speak with them directly about the system. Please contact me and I will forward their information to you. Hope this helps. Samir Dandekar The Technology Integration Group 919-461-2357 919-461-2358 fax http://www.ttig.com -----Original Message----- From: bullockb@SPAMFOIL.pt.cyanamid.com [SMTP:bullockb@SPAMFOIL.pt.cyanamid.com] Sent: Thursday, November 11, 1999 11:20 AM To: Lab-Robotics Discussion Mailing List Subject: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA --------------------- Lab-Robotics Discussion --------------------- Hello, I have been asked by our Biotech department to test the validity of my Tecan Gensis liquid handler to transfer DNA samples from 96 well plates to 384 well plates. I have various fixed needle types as well as disposable tips. I am sure that the problems I am running into are the same that many have already experienced.These are: 1. Because of the length of the fixed tips, even a tiny deviation from being off center will cause the needle to miss the well in the 384 well plate. I can not get the needles to hit the wells continously without crashing into the plate bending the needles. 2. With the disposable tips, guaranteeing that the tips will seat themselves prefectly every time is not likely resulting in the same problem as above. 3. If disposable tip are not used how do you prevent carry over of the DNA samples from well to well since DNA loves to bind to plastics and metals? Is simple solvent washing of the needles enough to prevent carry over or is there a cocktail that can be used to pre-wash with first? I have contacted Popper & Sons to have some fixed needles made that are only an inch or two long. This may help in solving the offset of the tip but I can't be sure until I try. Any help would be much appreciative. Thanks. Billy Bullock Project Leader - Automated Synthesis American Cyanamid Co. PO Box 400 Princeton, NJ 08543 --- Lab-Robotics Discussion The Lab-Robotics Discussion Mailing List is a feature of the Laboratory Robotics Interest Group, www.lab-robotics.org. From: James Schmeits Subject: Re: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Thu, 11 Nov 1999 13:42:49 -0800 >--------------------- >Lab-Robotics Discussion >--------------------- > Hello, > > I have been asked by our Biotech department to test the validity of my > Tecan Gensis liquid handler to transfer DNA samples from 96 well > plates to 384 well plates. I have various fixed needle types as well > as disposable tips. > > I am sure that the problems I am running into are the same that many > have already experienced.These are: > > 1. Because of the length of the fixed tips, even a tiny deviation from > being off center will cause the needle to miss the well in the 384 > well plate. I can not get the needles to hit the wells continously > without crashing into the plate bending the needles. Which can definitely get expensive over time. > 2. With the disposable tips, guaranteeing that the tips will seat > themselves prefectly every time is not likely resulting in the same > problem as above. > > 3. If disposable tip are not used how do you prevent carry over of the > DNA samples from well to well since DNA loves to bind to plastics and > metals? Is simple solvent washing of the needles enough to prevent > carry over or is there a cocktail that can be used to pre-wash with > first? I tried disposable tips and I found that the accuracy of liquid detection and handling just wasn't consistent. This was solved when I switched to standard tips. As for carryover of DNA, a simple water wash has proven to be enough for something like yeast DNA. For more sensitive applications, like human DNA for PCR amplification, I've included a bleach wash between samples. > I have contacted Popper & Sons to have some fixed needles made that > are only an inch or two long. This may help in solving the offset of > the tip but I can't be sure until I try. I haven't tried 384 wells yet, though I will soon. I thought that Tecan made a tip which was about 2 inches long and it seems pretty accurate. James Schmeits jschmeits@SPAMFOIL.ucsd.edu From: "Regina Gorski" Subject: Long-term Storage Date: Thu, 11 Nov 1999 16:39:37 -0500 I'm interested in long-term storage of DNA samples. Currently, we're looking at the HomeBase storage, the Mega-Stor from Tom Tec, and the Mol Bank from Tecan. I was wondering if anyone has any experience with these products, information about your current storage equipment, or if you can suggest any other products. Our requirements are to ideally store 1 million samples, but I'm willing to go with something smaller, to use microtubes in deepwells and/or microtiter plates, to track samples with barcodes, and to know the position of each sample in the storage. The samples also need to be easily accessible. We would also like to integrate it with our Tecan. Any advice or suggestions would be helpful. Thanks Regina Gorski Genomics Collaborative, Inc. 99 Erie St. Cambridge, MA 02139 tel# 617-661-2400 x224 fax# 617-661-8899 rgorski@SPAMFOIL.genecoop.com From: FisherJA@SPAMFOIL.pebio.com Subject: Re: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Thu, 11 Nov 1999 14:02:08 -0800 PE Biosystems' ABI Prism 877 uses a single stainless steel tip for transferring reagents, including DNA samples, for sequencing and PCR, as well as moving products after PCR. We clean the tip between samples by aspirating sodium hypochlorite, holding for about 20 sec, and then flushing thoroughly with water. We've found it to be effective at preventing sample-to-sample DNA carryover. We aspirate greater than the volume of DNA that was handled. Also we recommend using lab grade 5% hypochlorite (e.g. J.T. Baker) and NOT using household bleach like Clorox, mainly because we don't know what else is in it. Jim Fisher PE Biosystems Genetic Analysis R&D bullockb@SPAMFOIL.pt.cyanamid.com on 11/11/99 09:20:00 AM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: James A Fisher/FOS/PEC) Subject: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA --------------------- Lab-Robotics Discussion --------------------- Hello, I have been asked by our Biotech department to test the validity of my Tecan Gensis liquid handler to transfer DNA samples from 96 well plates to 384 well plates. I have various fixed needle types as well as disposable tips. I am sure that the problems I am running into are the same that many have already experienced.These are: 1. Because of the length of the fixed tips, even a tiny deviation from being off center will cause the needle to miss the well in the 384 well plate. I can not get the needles to hit the wells continously without crashing into the plate bending the needles. 2. With the disposable tips, guaranteeing that the tips will seat themselves prefectly every time is not likely resulting in the same problem as above. 3. If disposable tip are not used how do you prevent carry over of the DNA samples from well to well since DNA loves to bind to plastics and metals? Is simple solvent washing of the needles enough to prevent carry over or is there a cocktail that can be used to pre-wash with first? I have contacted Popper & Sons to have some fixed needles made that are only an inch or two long. This may help in solving the offset of the tip but I can't be sure until I try. Any help would be much appreciative. Thanks. Billy Bullock Project Leader - Automated Synthesis American Cyanamid Co. PO Box 400 Princeton, NJ 08543 --- Lab-Robotics Discussion To subscribe to this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just SUBSCRIBE as the subject. Alternatively, click on this link: mailto:discussion@SPAMFOIL.lab-robotics.org?subject=subscribe From: "Mike Brown" Subject: RE: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Thu, 11 Nov 1999 22:02:17 -0500 Billy: Tecan does have a shorter fixed tip (about 2 inches) that was developed specifically for use in 384 well applications. It's teflon coated stainless steel. I haven't used them yet, but I have seen them. Give Kelly McKeon a shout at Tecan US for additional information. I'd give you the part number if I could recall it, but I can't. If you don't have any luck, I'll be glad to help you dig out the information. Robert M. "Mike" Brown Jr. The Technology Integration Group, LLC (919) 461-2357 (919) 461-2358 (fax) http://www.ttig.com > -----Original Message----- > From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On > Behalf Of bullockb@SPAMFOIL.pt.cyanamid.com > Sent: Thursday, November 11, 1999 12:20 PM > To: Lab-Robotics Discussion Mailing List > Subject: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA > > > --------------------- > Lab-Robotics Discussion > --------------------- > Hello, > > I have been asked by our Biotech department to test the > validity of my > Tecan Gensis liquid handler to transfer DNA samples from 96 well > plates to 384 well plates. I have various fixed needle types as well > as disposable tips. > > I am sure that the problems I am running into are the same that many > have already experienced.These are: > > 1. Because of the length of the fixed tips, even a tiny > deviation from > being off center will cause the needle to miss the well in the 384 > well plate. I can not get the needles to hit the wells continously > without crashing into the plate bending the needles. > > 2. With the disposable tips, guaranteeing that the tips will seat > themselves prefectly every time is not likely resulting in the same > problem as above. > > 3. If disposable tip are not used how do you prevent carry > over of the > DNA samples from well to well since DNA loves to bind to > plastics and > metals? Is simple solvent washing of the needles enough to prevent > carry over or is there a cocktail that can be used to pre-wash with > first? > > I have contacted Popper & Sons to have some fixed needles made that > are only an inch or two long. This may help in solving the offset of > the tip but I can't be sure until I try. > > Any help would be much appreciative. Thanks. > > Billy Bullock > Project Leader - Automated Synthesis > American Cyanamid Co. > PO Box 400 > Princeton, NJ 08543 > > --- Lab-Robotics Discussion > To review archived messages from this list, send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just RESEND-xx (xx is a number) as > the subject. You will get a single digest message for the last xx > days of messages. > > From: "david allen" Subject: RE: Lab-Robotics Discussion: Long-term Storage Date: Fri, 12 Nov 1999 08:42:39 -0500 > samples, but I'm willing to go with something smaller, to use > microtubes in > deepwells and/or microtiter plates, to track samples with barcodes, and to > know the position of each sample in the storage. The samples also need to > be easily accessible. We would also like to integrate it with our Tecan. > Any advice or suggestions would be helpful. Thanks > you might want to check out Matrix. they're coming out with pre-labeled micro tubes in the SBS 96 well format racks. the labels are 2D barcodes with text, mounted on the bottom so they can be easily read. they're using a 10 digit code so the numbers will only repeat every 10^10th tube. the racks have a positive latch and appear rugged enough for shipping. not sure what sizes they'll offer, but .7 and 1.4ml seem certain. the tubes may be sealed with septa plugs that can be pierced by the Tecan. if you're concerned about tracking temp., I'm working on incorporating a data logger onto the 96 well racks. the target spec is 1 datapoint per hour for a year in the range of -20 to 85 deg. C. I've found all the parts, just looking for money and opportunity to put it together. da From: "Feiglin, Marc" Subject: RE: Lab-Robotics Discussion: Long-term Storage Date: Fri, 12 Nov 1999 08:54:29 -0500 Regina, We currently own a Tomtec MegaStor and expect delivery of a MolBank in a few weeks too. (We actuallly had ordered a Molbank years ago when it was known as a Cocoon, the copmany selling it then went out of business.) I can talk to you about these units if you want to contact me directly. You might also want to look at the comPOUND from Technology Partnership. Marc -----Original Message----- From: Regina Gorski [mailto:RGorski@SPAMFOIL.genecoop.com] Sent: Thursday, November 11, 1999 4:40 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Long-term Storage --------------------- Lab-Robotics Discussion --------------------- I'm interested in long-term storage of DNA samples. Currently, we're looking at the HomeBase storage, the Mega-Stor from Tom Tec, and the Mol Bank from Tecan. I was wondering if anyone has any experience with these products, information about your current storage equipment, or if you can suggest any other products. Our requirements are to ideally store 1 million samples, but I'm willing to go with something smaller, to use microtubes in deepwells and/or microtiter plates, to track samples with barcodes, and to know the position of each sample in the storage. The samples also need to be easily accessible. We would also like to integrate it with our Tecan. Any advice or suggestions would be helpful. Thanks Regina Gorski Genomics Collaborative, Inc. 99 Erie St. Cambridge, MA 02139 tel# 617-661-2400 x224 fax# 617-661-8899 rgorski@SPAMFOIL.genecoop.com --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: "Sheldon, Adrian" Subject: RE: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Fri, 12 Nov 1999 08:58:03 -0500 Your idea about much shorter needles sounds good, but I have had a good experience in using the disposable (conductive) Tecan tips in 384 well plates. As long as the well positions are taught carefully initially, the tips won't hit the walls. Adrian Sheldon Group Leader, HTS ARQule, Inc. > ---------- > From: bullockb@SPAMFOIL.pt.cyanamid.com[SMTP:bullockb@SPAMFOIL.pt.cyanamid.com] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Thursday, November 11, 1999 12:20 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA > > --------------------- > Lab-Robotics Discussion > --------------------- > Hello, > > I have been asked by our Biotech department to test the validity of > my > Tecan Gensis liquid handler to transfer DNA samples from 96 well > plates to 384 well plates. I have various fixed needle types as well > as disposable tips. > > I am sure that the problems I am running into are the same that many > have already experienced.These are: > > 1. Because of the length of the fixed tips, even a tiny deviation > from > being off center will cause the needle to miss the well in the 384 > well plate. I can not get the needles to hit the wells continously > without crashing into the plate bending the needles. > > 2. With the disposable tips, guaranteeing that the tips will seat > themselves prefectly every time is not likely resulting in the same > problem as above. > > 3. If disposable tip are not used how do you prevent carry over of > the > DNA samples from well to well since DNA loves to bind to plastics and > > metals? Is simple solvent washing of the needles enough to prevent > carry over or is there a cocktail that can be used to pre-wash with > first? > > I have contacted Popper & Sons to have some fixed needles made that > are only an inch or two long. This may help in solving the offset of > the tip but I can't be sure until I try. > > Any help would be much appreciative. Thanks. > > Billy Bullock > Project Leader - Automated Synthesis > American Cyanamid Co. > PO Box 400 > Princeton, NJ 08543 > > --- Lab-Robotics Discussion > There is a web based companion to the LRIG Discussion Mailing List > called DiscussWeb. The LRIG DiscussWeb allows for on-line messaging, > responses, and reviewing of past posts. It may be found at > https://www.lab-robotics.org/Web_Discuss/ > From: "david allen" Subject: RE: Lab-Robotics Discussion: Long-term Storage Date: Fri, 12 Nov 1999 11:30:33 -0500 > I'm interested in long-term storage of DNA samples. Currently, we're > looking at the HomeBase storage, the Mega-Stor from Tom Tec, and the Mol > Bank from Tecan. I was wondering if anyone has any experience with these > products, information about your current storage equipment, or if you can > suggest any other products. you may want to look at TekCel as well. at the last LRIG meeting in Boston, they were showing a very impressive storage system. it's highly modular and easily expanded as your needs increase. da From: Lynn Petukhova Subject: Re: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Fri, 12 Nov 1999 12:42:38 -0500 Hi: I have very recently begun using a Tecan Genesis? By teaching, do you mean defining x-value and y-value offsets in carrier definitions? Is it common for there to be variances between positions (+/- 0.5, but as high as 2). Once these values are defined, will they ever need to be re-defined (given the same carrier)? Thanks in advance. Lynn >--------------------- >Lab-Robotics Discussion >--------------------- >Your idea about much shorter needles sounds good, but I have had a good >experience in using the disposable (conductive) Tecan tips in 384 well >plates. As long as the well positions are taught carefully initially, the >tips won't hit the walls. > >Adrian Sheldon >Group Leader, HTS >ARQule, Inc. Lynn Orvieto Petukhova Genotyping Group Leader Starr Center for Human Genetics Rockefeller University 1230 York Avenue, Box 241 New York, NY 10021 phone: (212) 327-7181 fax: (212) 327-7182 From: "Suzanne B. Shepard" Subject: Re: Lab-Robotics Discussion: Long-term Storage Date: Fri, 12 Nov 1999 16:37:24 -0500 Dear Regina, Matrix Technologies has recently introduced TrakMates, our 1.4 ml Tube Rack System With Unique Identifiers for long term sample storage. These tubes are in 96 well format and are designed to work in robotic storage systems. The bottom of each tube has a 2-D barcode and a human readable code that has been laser engraved onto the tube. The features of this matrix code include the potential for 10 billion unique codes and, most importantly, the impossibility of a misread in the code. The rack allows easy access to individual tubes and a locking mechanism for secure storage. I believe TrakMates is a good fit for you. Please call me @SPAMFOIL. 800.345.0206 x 161 for further information. Sincerely, Suzanne B. Shepard Sales Representative Matrix Technologies 22 Friars Drive Hudson, NH 800.345.0206 x 161 Phone 603.595.0106 Fax www.matrixtechcorp.com sbshepard@SPAMFOIL.excelonline.com -----Original Message----- From: Regina Gorski To: Lab-Robotics Discussion Mailing List Date: Friday, November 12, 1999 12:12 AM Subject: Lab-Robotics Discussion: Long-term Storage >--------------------- >Lab-Robotics Discussion >--------------------- >I'm interested in long-term storage of DNA samples. Currently, we're >looking at the HomeBase storage, the Mega-Stor from Tom Tec, and the Mol >Bank from Tecan. I was wondering if anyone has any experience with these >products, information about your current storage equipment, or if you can >suggest any other products. Our requirements are to ideally store 1 million >samples, but I'm willing to go with something smaller, to use microtubes in >deepwells and/or microtiter plates, to track samples with barcodes, and to >know the position of each sample in the storage. The samples also need to >be easily accessible. We would also like to integrate it with our Tecan. >Any advice or suggestions would be helpful. Thanks > >Regina Gorski >Genomics Collaborative, Inc. >99 Erie St. >Cambridge, MA 02139 >tel# 617-661-2400 x224 >fax# 617-661-8899 >rgorski@SPAMFOIL.genecoop.com > >--- Lab-Robotics Discussion >The Lab-Robotics Discussion Mailing List is a feature of the >Laboratory Robotics Interest Group, www.lab-robotics.org. > From: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion) Subject: Thank you for subscribing to Lab-Robotics Discussion Date: Mon, 15 Nov 1999 12:20:10 -0500 Thank you for subscribing to the Lab-Robotics Discussion mailing list. This message is sent once every 30 days to remind you of the commands which Arrow (our mailing list software) understands. When you reply to a message in a forum like this, it is generally considered "netiquette" to trim the original message down to just the portion you are replying to (if possible). Also, if you are replying to a string of messages, the same principle applies. The aim is to reduce the clutter for the other list members while preserving the sense of the message. When you post to the list, you may receive back some autoreplies from other list members email systems. This happens because some mail systems incorrectly use the "From:" heading instead of the "Reply To:" heading for autoreplies. This is unavoidable for us since we want to know who is sending a message to our list. To unsubscribe from the list, send a message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. To change to digest mode and receive messages once a day send a message with DIGEST-1 as the subject. To receive a digest once a week, send a message with a subject of DIGEST-7. To change from digest mode back to individual messages, send a message with DIGEST-OFF as the subject. For help with these and other Arrow commands, send a message to discussion@SPAMFOIL.lab-robotics.org with just HELP as the subject. Note: All Arrow commands are a single word (with no spaces) and are sent in the subject of the message to the list. Andy Zaayenga Moderator, The Lab-Robotics Discussion Mailing List mailto:andy.zaayenga@SPAMFOIL.lab-robotics.org web site: https://www.lab-robotics.org --- Age of List in Days 414 Number of Subscribers 802 Total Number of Messages Posted to List 1029 Average Number of Messages Posted Per Day 2 From: bullockb@SPAMFOIL.pt.cyanamid.com Subject: 96 to 384 Transfer Date: Wed, 17 Nov 1999 09:53 -0400 (EDT) Hello All, Last week I wrote about some problems I was having reformatting 96 well plates into 384 well plates using my Tecan Genesis. First I want to thank everyone who wrote back, many with very good ideas. Second I want to say that Tecan came to my rescue with low volume fixed needles that can be individually adjusted to hit the wells of a 384 well plate. Once adjusted properly, the system did a great job of doing the transfers as well as tracking up from the bottom of the 384 well so that an air bubble is not introduced into the well. This is definitely the place to come for answers. Best Regards, Billy Bullock Project Leader - Automated Synthesis Dept. American Cyanamid Co. From: "Waqas Khalid" Subject: Mechanical Engineering student Date: Tue, 23 Nov 1999 03:05:21 +0500 Hi, this is my first email to this group. I am a Mechanical Engineering students, and I would really appreciate some help for my first robot. I need all the info i can get, plus , if u can tell me of some good sites, I would really appreciate it. Waqas From: Sally Dowling Subject: RE: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA Date: Wed, 17 Nov 1999 18:13:12 -0600 good luck, call an engineering based company to help. >---------- >From: Sheldon, Adrian[SMTP:ASheldon@SPAMFOIL.arqule.com] >Sent: Friday, November 12, 1999 7:58 AM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA > >--------------------- >Lab-Robotics Discussion >--------------------- >Your idea about much shorter needles sounds good, but I have had a good >experience in using the disposable (conductive) Tecan tips in 384 well >plates. As long as the well positions are taught carefully initially, the >tips won't hit the walls. > >Adrian Sheldon >Group Leader, HTS >ARQule, Inc. >> ---------- >> From: bullockb@SPAMFOIL.pt.cyanamid.com[SMTP:bullockb@SPAMFOIL.pt.cyanamid.com] >> Reply To: discussion@SPAMFOIL.lab-robotics.org >> Sent: Thursday, November 11, 1999 12:20 PM >> To: discussion@SPAMFOIL.lab-robotics.org >> Subject: Lab-Robotics Discussion: 96 well to 384 well transfer of DNA >> >> --------------------- >> Lab-Robotics Discussion >> --------------------- >> Hello, >> >> I have been asked by our Biotech department to test the validity of >> my >> Tecan Gensis liquid handler to transfer DNA samples from 96 well >> plates to 384 well plates. I have various fixed needle types as well >> as disposable tips. >> >> I am sure that the problems I am running into are the same that many >> have already experienced.These are: >> >> 1. Because of the length of the fixed tips, even a tiny deviation >> from >> being off center will cause the needle to miss the well in the 384 >> well plate. I can not get the needles to hit the wells continously >> without crashing into the plate bending the needles. >> >> 2. With the disposable tips, guaranteeing that the tips will seat >> themselves prefectly every time is not likely resulting in the same >> problem as above. >> >> 3. If disposable tip are not used how do you prevent carry over of >> the >> DNA samples from well to well since DNA loves to bind to plastics and >> >> metals? Is simple solvent washing of the needles enough to prevent >> carry over or is there a cocktail that can be used to pre-wash with >> first? >> >> I have contacted Popper & Sons to have some fixed needles made that >> are only an inch or two long. This may help in solving the offset of >> the tip but I can't be sure until I try. >> >> Any help would be much appreciative. Thanks. >> >> Billy Bullock >> Project Leader - Automated Synthesis >> American Cyanamid Co. >> PO Box 400 >> Princeton, NJ 08543 >> >> --- Lab-Robotics Discussion >> There is a web based companion to the LRIG Discussion Mailing List >> called DiscussWeb. The LRIG DiscussWeb allows for on-line messaging, >> responses, and reviewing of past posts. It may be found at >> https://www.lab-robotics.org/Web_Discuss/ >> > >--- Lab-Robotics Discussion >To be removed from this list, please send an e-mail message to >discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. >Alternatively, click on this link >mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > > From: Sally Dowling Subject: RE: Lab-Robotics Discussion: Long-term Storage Date: Wed, 17 Nov 1999 18:13:20 -0600 hey, there is a theme here.... doesn't.........support applications? MTAC--that is Mettler Toledo Automated Chemistry does.' Sorry for the Advert... that is not my style, but this is getting to be a theme and I could either bow out of the discussion group or reply,.... >---------- >From: Feiglin, Marc[SMTP:marc_feiglin@SPAMFOIL.merck.com] >Sent: Friday, November 12, 1999 7:54 AM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: Long-term Storage > >--------------------- >Lab-Robotics Discussion >--------------------- >Regina, >We currently own a Tomtec MegaStor and expect delivery of a MolBank in a few >weeks too. (We actually had ordered a Molbank years ago when it was known >as a Cocoon, the copmany selling it then went out of business.) I can talk >to you about these units if you want to contact me directly. >You might also want to look at the comPOUND from Technology Partnership. >Marc > >-----Original Message----- >From: Regina Gorski [mailto:RGorski@SPAMFOIL.genecoop.com] >Sent: Thursday, November 11, 1999 4:40 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: Long-term Storage > > >--------------------- >Lab-Robotics Discussion >--------------------- >I'm interested in long-term storage of DNA samples. Currently, we're >looking at the HomeBase storage, the Mega-Stor from Tom Tec, and the Mol >Bank from Tecan. I was wondering if anyone has any experience with these >products, information about your current storage equipment, or if you can >suggest any other products. Our requirements are to ideally store 1 million >samples, but I'm willing to go with something smaller, to use microtubes in >deepwells and/or microtiter plates, to track samples with barcodes, and to >know the position of each sample in the storage. The samples also need to >be easily accessible. We would also like to integrate it with our Tecan. >Any advice or suggestions would be helpful. Thanks > >Regina Gorski >Genomics Collaborative, Inc. >99 Erie St. >Cambridge, MA 02139 >tel# 617-661-2400 x224 >fax# 617-661-8899 >rgorski@SPAMFOIL.genecoop.com > From: Sally Dowling Subject: RE: Lab-Robotics Discussion: 1536 absorbance & fluorescencereader Date: Wed, 17 Nov 1999 18:14:02 -0600 oh, great, another vendor..... >---------- >From: David Hitrys[SMTP:dhitrys@SPAMFOIL.world.std.com] >Sent: Thursday, November 11, 1999 10:56 AM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: 1536 absorbance & fluorescencereader > >--------------------- >Lab-Robotics Discussion >--------------------- >Check out the new reader from Carl Zeiss. It is distributed in the U.S. >by Cellomics, Inc. > >A lot more can be found at: > >http://www.cellomics.com/html/products/uhts_instrumentation.htm > >I know a little more about it and will be pleased to answer your >questions. >I can be reached at 508-877-5668. > >David Hitrys > > > > >Suzanne Martinez wrote: >> >> --------------------- >> Lab-Robotics Discussion >> --------------------- >> Johannes, >> Unfortunately, Molecular Devices has not yet developed a 1536 platereader >>or fluorescence reader. However, I would like to mention that we just >>started to sell a 1536 platewasher(the first 1536 washer on the market), >>called the Embla 1536. If you are >interested, please send inquiries to info@SPAMFOIL.moldev.com. >> >> Suzanne Martinez >> Skatron Product Manager >> Molecular Devices Corp. >> 1311 Orleans Drive >> Sunnyvale, CA 94089 >> Phone: 408-548-6305, 800-635-5577 >> Fax: 408-747-3602 >> Email: Suzanne_Martinez@SPAMFOIL.MolDev.com >> >> >>> "JJF (Johannes Fels)" 11/08/99 02:38AM >>> >> --------------------- >> Lab-Robotics Discussion >> --------------------- >> I am just curious, if somebody knows vendors selling absorbance & >> fluorescence readers capable to read 1536 micro titer plates. >> I am aware of the BMG and the Tecan readers. >> >> Regards, >> >> Johannes Fels >> From: Sally Dowling Subject: RE: Lab-Robotics Discussion: 96 well concentrator Date: Wed, 17 Nov 1999 18:14:07 -0600 hey,Rob, you are a great guy, but I am refraining from Adverts on this site, and I think we all should respond to the people personally, or I am not delighted to sign on after vacation with more tha half of my email from us all.... ..... ... >---------- >From: Rob Maddox[SMTP:rob.maddox@SPAMFOIL.genevacusa.com] >Sent: Wednesday, October 27, 1999 8:42 AM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: 96 well concentrator > >--------------------- >Lab-Robotics Discussion >--------------------- >Genevac makes a series of instruments for evaporating plates and vials. For >further information our web site is www.genevac.com. > >Rob Maddox >Sales Manager >502-239-7704 > From: Sally Dowling Subject: RE: Lab-Robotics Discussion: 1536 absorbance & fluorescencereader Date: Wed, 17 Nov 1999 18:14:05 -0600 great, another vendor advert on the dicussion group... argggggghhhhhethhhh >---------- >From: Suzanne Martinez[SMTP:Suzanne_Martinez@SPAMFOIL.moldev.com] >Sent: Monday, November 08, 1999 10:23 AM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: 1536 absorbance & fluorescencereader > >--------------------- >Lab-Robotics Discussion >--------------------- >Johannes, >Unfortunately, Molecular Devices has not yet developed a 1536 platereader or >fluorescence reader. However, I would like to mention that we just started >to sell a 1536 platewasher(the first 1536 washer on the market), called the >Embla 1536. If you are interested, please send inquiries to info@SPAMFOIL.moldev.com. > >Suzanne Martinez >Skatron Product Manager >Molecular Devices Corp. >1311 Orleans Drive >Sunnyvale, CA 94089 >Phone: 408-548-6305, 800-635-5577 >Fax: 408-747-3602 >Email: Suzanne_Martinez@SPAMFOIL.MolDev.com > >>>> "JJF (Johannes Fels)" 11/08/99 02:38AM >>> >--------------------- >Lab-Robotics Discussion >--------------------- >I am just curious, if somebody knows vendors selling absorbance & >fluorescence readers capable to read 1536 micro titer plates. >I am aware of the BMG and the Tecan readers. > >Regards, > >Johannes Fels > >--- Lab-Robotics Discussion >To review archived messages from this list, send an e-mail message to >discussion@SPAMFOIL.lab-robotics.org with just RESEND-xx (xx is a number) as >the subject. You will get a single digest message for the last xx >days of messages. > >--- Lab-Robotics Discussion >To be removed from this list, please send an e-mail message to >discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. >Alternatively, click on this link >mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > From: Hank20000@SPAMFOIL.aol.com Subject: 384 Pipette Racks Date: Wed, 17 Nov 1999 22:00:11 EST I am Interested to see if there is a manufacturer or company that has a 384 formatted disposable pipette tip rack. If any body knows of one, could you please tell me who sells it and if it is specifically designed to be used with a certain robotic pipetting system. Thank you in advance. Hank K. Denville Scientific From: Sam Pickering Subject: Glass 6-well Date: Thu, 18 Nov 1999 11:52:39 +0000 Does anybody know where I can get a glass 6-well plate from? I need to make some modifications to the inside of the wells, and then autoclave (121 degrees Centigrade, one bar guage). I don't want to have to gamma-irradiate, and all that I've found so far is polystyrene/ethylene/propylene/carbonate. Glass seems to only be used in the manufacture of 96-wells or more. Any help would be much appreciated, Sam Pickering. From: Robert F Trinka Subject: Lab-Robotics Discussion: 384 Pipette Racks Date: Thu, 18 Nov 1999 11:20:40 -0500 Lab Systems had a rack of 384 tips. Hank, Another option is Molecular Devices that sells tips for their FLIPR 384. I belive Packard CCS will offer tips soon if they are not already. In addition, Matrix is launching their 384 head pipettor, however I have not seen their Rack. I hope this helps. Chad Jenkins E&K Scientific Products,Inc. (800) 934-8114 ext. 105 http://www.eandkscientific.com Hank20000@SPAMFOIL.aol.com wrote: --------------------- Lab-Robotics Discussion --------------------- I am Interested to see if there is a manufacturer or company that has a 384 formatted disposable pipette tip rack. If any body knows of one, could you please tell me who sells it and if it is specifically designed to be used with a certain robotic pipetting system. Thank you in advance. Hank K. Denville Scientific --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: David Subject: Re: Lab-Robotics Discussion: 96 well concentrator Date: Thu, 18 Nov 1999 23:34:32 +0000 Sally Dowling wrote: > hey,Rob, you are a great guy, but I am refraining from Adverts on this > site, and I think we all should respond to the people personally, or I > am not delighted to sign on after vacation with more tha half of my > email from us all.... ..... ... > >From: Rob Maddox[SMTP:rob.maddox@SPAMFOIL.genevacusa.com] > >Genevac makes a series of instruments for evaporating plates and vials. For > >further information our web site is www.genevac.com. I was thinking, does it really matter? Someone writes asking where they can get a certain product, a vendor writes back saying "yes, us", and gives a web site. We can hit delete or look at the web site. David (yes I know, I'm doing it too!) :) -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com Press any key to continue or any other key to quit... From: David Subject: Re: Lab-Robotics Discussion: Mechanical Engineering student Date: Thu, 18 Nov 1999 23:49:16 +0000 Waqas Khalid wrote: > > --------------------- > Lab-Robotics Discussion > --------------------- > Hi, this is my first email to this group. > I am a Mechanical Engineering students, and I would really appreciate some > help for my first robot. > > I need all the info i can get, plus , if u can tell me of some good sites, I > would really appreciate it. I remember what it was like trying to get info as a student. You are not going to get much of actual nuts and bolts because the technologies are closely guarded by the robot companies. But if you look on our site (below) you will see some diagrams which might help. Mainly search the web. David -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com Press any key to continue or any other key to quit... From: Martha Bowden Subject: RE: Lab-Robotics Discussion: Mechanical Engineering student Date: Thu, 18 Nov 1999 18:12:44 -0800 Check out the LabAutomation 2000 Conference site at www.labautomation.org Several of the vendors exhibiting are robotics companies. At least from there you can go to their web sites. Martha Bowden Argonaut Technologies (showing at lab automation but NOT a general purpose robotics company) mbowden@SPAMFOIL.argotech.com -----Original Message----- From: Waqas Khalid [mailto:u970127@SPAMFOIL.giki.edu.pk] Sent: Monday, November 22, 1999 2:05 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Mechanical Engineering student --------------------- Lab-Robotics Discussion --------------------- Hi, this is my first email to this group. I am a Mechanical Engineering students, and I would really appreciate some help for my first robot. I need all the info i can get, plus , if u can tell me of some good sites, I would really appreciate it. Waqas From: Sally Dowling Subject: RE: Lab-Robotics Discussion: 96 well concentrator Date: Fri, 19 Nov 1999 09:28:56 -0600 Point taken, I was reacting to >60% of emails after 5 days away being from LRIG Discussion group and >50% of them being this type of solicitation. Andy Z. had asked in the past that the vendors reply directly to the questioner because of the email deluge, and I am deluged so I may have overstated the case. I will resign from the discussion group, at the risk of missing pertinent updates to eliminate this problem. >---------- >From: David[SMTP:robotics1@SPAMFOIL.dial.pipex.com] >Sent: Thursday, November 18, 1999 5:34 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: 96 well concentrator > >--------------------- >Lab-Robotics Discussion >--------------------- >Sally Dowling wrote: >> hey,Rob, you are a great guy, but I am refraining from Adverts on this >> site, and I think we all should respond to the people personally, or I >> am not delighted to sign on after vacation with more tha half of my >> email from us all.... ..... ... >> >From: Rob Maddox[SMTP:rob.maddox@SPAMFOIL.genevacusa.com] >> >Genevac makes a series of instruments for evaporating plates and vials. >>For >> >further information our web site is www.genevac.com. > >I was thinking, does it really matter? Someone writes asking where they >can get a certain product, a vendor writes back saying "yes, us", and >gives a web site. We can hit delete or look at the web site. > >David >(yes I know, I'm doing it too!) :) >-- >________________________________________________________ > David N. Sands, ST Robotics International > Website: http://www.strobotics.com > >Press any key to continue or any other key to quit... > >--- Lab-Robotics Discussion >To view statistics on this list, send an e-mail message to >discussion@SPAMFOIL.lab-robotics.org with just STATS as the subject. You will >receive statistics for the mailing list showing number of >subscribers, domain types and messages. > > From: "Kevin Olsen" Subject: First Robot - help needed Date: Fri, 19 Nov 1999 10:29:54 -0500 Dear Mr. Khalid, I have a question about your post to the Laboratory Robotics Interest Group mailing list. What is the intended use of your robot? I suspect that most subscribers to the discussion list will tell you (and they will be speaking from sometimes painful experience) that the reliability of any robotic system is inversely proportional to the its complexity. Many laboratories would rather have smaller units that do one or two jobs very well, than larger units that can do many jobs with only average reliability. This is also true in most industrial settings. This is a rule that I am sure you already know. But everyone (including myself) in robotics is constantly tempted to break it. Good luck, please feel free to share what are learning with this community, Kevin Olsen Robotics & Automation Group Wyeth Ayerst Research Pearl River, New York, USA From: "Doug Gurevitch" Subject: Lab-Robotics Discussion: Mechanical Engineering student Date: Fri, 19 Nov 1999 09:44:09 -0800 Waqas, Check out the following resources from the general/industrial robotics world. Also, you may want to start with a kit robot to minimize cost and time sourcing parts. Arrick robotics Robot Information Central - Arrick makes automation components and kit robots - http://www.robotics.com/robots.html The Robotic Industries Association (RIA) - http://www.robotics.org/ The Institute for Electrical and Electronics Engineers Robotics and Automation Society (IEEE is the main robotics organization in the US) - http://www.ncsu.edu/IEEE-RAS/ Robix - educational kit robot - http://www.robix.com/ The Rockies Robotics Group - one of the better hobbyist web sites - http://www.rockies-robotics.com/ Good luck. Sincerely, Doug Gurevitch -----Original Message----- From: Waqas Khalid [mailto:u970127@SPAMFOIL.giki.edu.pk] Sent: Monday, November 22, 1999 2:05 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Mechanical Engineering student --------------------- Lab-Robotics Discussion --------------------- Hi, this is my first email to this group. I am a Mechanical Engineering students, and I would really appreciate some help for my first robot. I need all the info i can get, plus , if u can tell me of some good sites, I would really appreciate it. Waqas --- Lab-Robotics Discussion To review archived messages from this list, send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just RESEND-xx (xx is a number) as the subject. You will get a single digest message for the last xx days of messages. From: "Ramesha, Chakk {High~Palo Alto}" Subject: Radioactive Counters Y2K Date: Fri, 19 Nov 1999 14:44:22 -0800 Can anyone tell me the impact of NOT making the radioactive counters Y2K compliant. I have tried all sorts of things I cannot justify spending $60k to make few of them Y2K compliant. I see a problem if I use it for calculting decay, but I never use it for that purpose. Any knowledge in this regard is appreciated. Chakk From: David Subject: Re: Lab-Robotics Discussion: 96 well concentrator Date: Fri, 19 Nov 1999 21:13:40 +0000 Sally Dowling wrote: > Point taken, I was reacting to >60% of emails after 5 days away being > from LRIG Discussion group and >50% of them being this type of > solicitation. Andy Z. had asked in the past that the vendors reply > directly to the questioner because of the email deluge, and I am deluged > so I may have overstated the case. > > I will resign from the discussion group, at the risk of missing > pertinent updates to eliminate this problem. I don't think you should resign. The discussion group is still in it's infancy as I'm sure will be agreed in a few years time looking back. There are bound to be problems. The strangest one is the autoresponders. During the summer when many people were away I sent in one posting and got back no end of auto-respond messages. Presumably these in turn were relayed back to the auto-responders but it didn't seem to keep repeating. Maybe Andy knows why. David -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com Error reading FAT record: Try the SKINNY one? (Y/N) From: David Subject: Re: Lab-Robotics Discussion: Mechanical Engineering student Date: Fri, 19 Nov 1999 21:17:04 +0000 Doug Gurevitch wrote: > Waqas, > > Check out the following resources from the general/industrial robotics > world. Also, you may want to start with a kit robot to minimize cost > and time sourcing parts. Excellent information. Thanks for helping the student with these resources. I'll note them myself for future reference. David -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com Error reading FAT record: Try the SKINNY one? (Y/N) From: Laboratory Robotics Interest Group Subject: Re: Vendor Replies Date: Sat, 20 Nov 1999 16:02:27 -0500 Sally, It is a fine line between informing and unsolicited advertising as you point out. If someone asks a question about the availability of a particular product, I think it is fine if a vendor answers with details of a product of theirs that fits. The thing we would like to avoid is the "call me, we do everything" or "well, we don't have that, but we do have..." responses that are just irrelevant. Most of the responses were fairly on target given the nature of the questions. It really doesn't matter that a vendor or end user answers if it is a relevant reply. Many vendors do reply privately to posts. However, if it is a valid reply, this denies the rest of us the knowledge of what is out there. It's a tough call - we don't want to clog our email boxes with unsolicited advertising, but I think the vendor contributors to this list have been very good in their targeted replies. I welcome the subscribers' input, either publicly to the list or personally to my email address listed below. Andy Zaayenga Moderator, The Lab-Robotics Discussion Mailing List mailto:andy.zaayenga@SPAMFOIL.lab-robotics.org At 09:28 AM 11/19/1999 -0600, Sally Dowling wrote to Andy: >--------------------- >Lab-Robotics Discussion >--------------------- >Point taken, I was reacting to >60% of emails after 5 days away being >from LRIG Discussion group and >50% of them being this type of >solicitation. Andy Z. had asked in the past that the vendors reply >directly to the questioner because of the email deluge, and I am deluged >so I may have overstated the case. > >I will resign from the discussion group, at the risk of missing >pertinent updates to eliminate this problem. From: Laboratory Robotics Interest Group Subject: Re: Lab-Robotics Discussion: 96 well concentrator Date: Sat, 20 Nov 1999 17:15:58 -0500 David, The problem with the autoreplies happens because some mail systems incorrectly use the "From:" heading instead of the "Reply To:" heading for autoreplies. This is unavoidable for us since we want to know who is sending a message to our list. Andy At 09:13 PM 11/19/1999 +0000, David wrote to Andy: >The discussion group is still in it's >infancy as I'm sure will be agreed in a few years time looking back. >There are bound to be problems. The strangest one is the >autoresponders. During the summer when many people were away I sent in >one posting and got back no end of auto-respond messages. Presumably >these in turn were relayed back to the auto-responders but it didn't >seem to keep repeating. Maybe Andy knows why. > >David >-- >________________________________________________________ > David N. Sands, ST Robotics International > Website: http://www.strobotics.com From: David Subject: Re: Lab-Robotics Discussion: Radioactive Counters Y2K Date: Sat, 20 Nov 1999 23:09:53 +0000 Ramesha, Chakk {High~Palo Alto} wrote: > > --------------------- > Lab-Robotics Discussion > --------------------- > Can anyone tell me the impact of NOT making the radioactive counters Y2K > compliant. I have tried all sorts of things I cannot justify spending $60k > to make few of them Y2K compliant. I see a problem if I use it for > calculting decay, but I never use it for that purpose. Any knowledge in > this regard is appreciated. > > Chakk $60K to make counters Y2K compliant? I'm in the wrong business.! I don't know what make these counters are or how they work, but are they computer based? The acid test is: does it use the date and can you enter the date? The Y2K bug is limited to small systems which used only 2 digits to express the year, so 00 is less than 99. Such systems are almost all obsolete, for example a 286 computer that still uses DOS 4 or something like that. The Y2K is largely a scam, perpetuated by computer professionals who are making big bucks 'fixing' a problem which usually isn't there. It's the Emperor's new clothes. However, practically what you can do is simple: If it's a computer enter the date as December 31st 1999, time 23:58. Switch off the computer (or device), switch on again 3 minutes later and check what date and time it thinks it is, also check any software which uses date. If it's some other device (without battery clock) then set its date to 31-12-99, time 23:59 and watch. Later put the clocks back again. Of course, if the device does not use date or ask for date then it can't possibly have the Y2K bug. The only other problem the system might have is: does it recognise that year 2000 is a leap year. To test this set the date to 28 Feb 00 and see what happens. All this I say without any knowledge of your particular device. Perhaps you could provide more details. David -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com Error reading FAT record: Try the SKINNY one? (Y/N) From: "Ramesha, Chakk {High~Palo Alto}" Subject: RE: Lab-Robotics Discussion: Radioactive Counters Y2K Date: Sun, 21 Nov 1999 11:34:26 -0800 David, Thanks for the reply. It appeared to me like a scam too. That is why I decided to solicit some opinions. Yes, these counters are controlled by computers, but the counting is mostly done by PMT. The date functions appear mostly cosmetic. I did go through the routine like changing dates etc. to make sure that in Y2000 it continue to correctly count the radioactivity and report the cpm or dpm. Changing date had absolutely no effect either on the performance or on the absolute cpm/dpm counted and reported. I have several of these counters, some old some new. None of them showed any problems following the date changes. Once again thanks, Chakk > -----Original Message----- > From: David [SMTP:robotics1@SPAMFOIL.dial.pipex.com] > Sent: Saturday, November 20, 1999 3:10 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: Radioactive Counters Y2K > > --------------------- > Lab-Robotics Discussion > --------------------- > Ramesha, Chakk {High~Palo Alto} wrote: > > > > --------------------- > > Lab-Robotics Discussion > > --------------------- > > Can anyone tell me the impact of NOT making the radioactive counters Y2K > > compliant. I have tried all sorts of things I cannot justify spending > $60k > > to make few of them Y2K compliant. I see a problem if I use it for > > calculting decay, but I never use it for that purpose. Any knowledge in > > this regard is appreciated. > > > > Chakk > > $60K to make counters Y2K compliant? I'm in the wrong business.! > I don't know what make these counters are or how they work, but are they > computer based? > The acid test is: does it use the date and can you enter the date? > The Y2K bug is limited to small systems which used only 2 digits to > express the year, so 00 is less than 99. Such systems are almost all > obsolete, for example a 286 computer that still uses DOS 4 or something > like that. The Y2K is largely a scam, perpetuated by computer > professionals who are making big bucks 'fixing' a problem which usually > isn't there. It's the Emperor's new clothes. > However, practically what you can do is simple: If it's a computer enter > the date as December 31st 1999, time 23:58. Switch off the computer (or > device), switch on again 3 minutes later and check what date and time it > thinks it is, also check any software which uses date. If it's some > other device (without battery clock) then set its date to 31-12-99, time > 23:59 and watch. Later put the clocks back again. Of course, if the > device does not use date or ask for date then it can't possibly have the > Y2K bug. > > The only other problem the system might have is: does it recognise that > year 2000 is a leap year. To test this set the date to 28 Feb 00 and see > what happens. > > All this I say without any knowledge of your particular device. Perhaps > you could provide more details. > > David > > -- > ________________________________________________________ > David N. Sands, ST Robotics International > Website: http://www.strobotics.com > > Error reading FAT record: Try the SKINNY one? (Y/N) > From: Randall Engler Subject: Lab-Robotics Discussion: Vendor Replies Date: Sun, 21 Nov 1999 15:31:11 -0800 Andy: Thanks for an accurate and coherent position on vendor participation in the discussion group. If a vendor has products or information that are relevant to the question or discussion, it is absolutely appropriate for a vendor rep to respond with specific information (including other contact information: phone, fax, etc.). And, as this is a dynamic and competitive area of technology development, there may be many such replies to a query for product, method or service information. Comes with the territory. If one feels that some e-mails from this discussion are inappropriate, there are filters for e-mail systems. Such systems are rigid, however, and may be difficult to use to address this issue: for example, if you filter out .com e-mail addresses, you will miss 98% of the discussion. Randy Laboratory Robotics Interest Group wrote: > --------------------- > Lab-Robotics Discussion > --------------------- > Sally, > > It is a fine line between informing and unsolicited advertising as you point out. If someone asks a question about the availability of a particular product, I think it is fine if a vendor answers with details of a product of theirs that fits. The thing we would like to avoid is the "call me, we do everything" or "well, we don't have that, but we do have..." responses that are just irrelevant. Most of the responses were fairly on target given the nature of the questions. It really doesn't matter that a vendor or end user answers if it is a relevant reply. Many vendors do reply privately to posts. However, if it is a valid reply, this denies the rest of us the knowledge of what is out there. It's a tough call - we don't want to clog our email boxes with unsolicited advertising, but I think the vendor contributors to this list have been very good in their targeted replies. I welcome the subscribers' input, either publicly to the list or personally to my email address listed below. > > Andy Zaayenga > Moderator, The Lab-Robotics Discussion Mailing List > mailto:andy.zaayenga@SPAMFOIL.lab-robotics.org > > At 09:28 AM 11/19/1999 -0600, Sally Dowling wrote to Andy: > >--------------------- > >Lab-Robotics Discussion > >--------------------- > >Point taken, I was reacting to >60% of emails after 5 days away being > >from LRIG Discussion group and >50% of them being this type of > >solicitation. Andy Z. had asked in the past that the vendors reply > >directly to the questioner because of the email deluge, and I am deluged > >so I may have overstated the case. > > > >I will resign from the discussion group, at the risk of missing > >pertinent updates to eliminate this problem. > From: Jackbonham@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: Radioactive Counters Y2K Date: Sun, 21 Nov 1999 22:50:43 EST Chakk, It is unlikely that you have a counter that will not function after the new year. Gamma counters, however, fall under FDA regulations for Medical Devices as they may be used in in-vitro diagnostic work and the manufacturer may have stated that either your unit or the software you are using to control it is not fully compliant by the FDA guidelines. There are very specific regulations regarding "compliant" vs. "noncompliant." Stored curves, backgrounds, normalizations, etc., may be confused in the software over the 1999-2000 transition but there are several workarounds, if there is not an outright fix to the software. Most counters are "dumb" detection devices and use a control device (computer-internal or external or teletype) to control its operation. If the detectors are in good shape there should be a fix. Jack Bonham Titertek Instruments, Inc. From: "John Morin" Subject: Re: Lab-Robotics Discussion: Vendor Replies Date: Mon, 22 Nov 1999 10:09:21 -0500 Andy, I'm posting this to the public pool to offer support for your position that it is perfectly appropriate for a vendor to offer a relevant response to a question posted on this web site. I have found this site to be interesting, entertaining and informative and don't begrudge the approximately 5 seconds total elapsed time it takes to open each e-mail, scan the content and either absorb it or consign it to oblivion. Thanks to everyone who has supported this educational opportunity. John Morin Wyeth Research High Throughput Screening >>> Laboratory Robotics Interest Group 11/20 4:02 PM >>> --------------------- Lab-Robotics Discussion --------------------- Sally, It is a fine line between informing and unsolicited advertising as you point out. If someone asks a question about the availability of a particular product, I think it is fine if a vendor answers with details of a product of theirs that fits. The thing we would like to avoid is the "call me, we do everything" or "well, we don't have that, but we do have..." responses that are just irrelevant. Most of the responses were fairly on target given the nature of the questions. It really doesn't matter that a vendor or end user answers if it is a relevant reply. Many vendors do reply privately to posts. However, if it is a valid reply, this denies the rest of us the knowledge of what is out there. It's a tough call - we don't want to clog our email boxes with unsolicited advertising, but I think the vendor contributors to this list have been very good in their targeted replies. I welcome the subscribers' input, either publicly to the list or personally to my email address listed below. Andy Zaayenga Moderator, The Lab-Robotics Discussion Mailing List mailto:andy.zaayenga@SPAMFOIL.lab-robotics.org At 09:28 AM 11/19/1999 -0600, Sally Dowling wrote to Andy: >--------------------- >Lab-Robotics Discussion >--------------------- >Point taken, I was reacting to >60% of emails after 5 days away being >from LRIG Discussion group and >50% of them being this type of >solicitation. Andy Z. had asked in the past that the vendors reply >directly to the questioner because of the email deluge, and I am deluged >so I may have overstated the case. > >I will resign from the discussion group, at the risk of missing >pertinent updates to eliminate this problem. From: "Birzin, Elizabeth T." Subject: RE: Lab-Robotics Discussion: Radioactive Counters Y2K Date: Mon, 22 Nov 1999 10:05:09 -0500 My chief concern was for documentation purposes. If one ever gets into a patent argument with another company, It may be disadvantageous to have documents with incorrect dates. Also, notebooks are collected for FDA submissions. My feeling is better safe than sorry. > ---------- > From: Ramesha, Chakk {High~Palo > Alto}[SMTP:CHAKK.RAMESHA@SPAMFOIL.ROCHE.COM] > Sent: Friday, November 19, 1999 5:44 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: Radioactive Counters Y2K > > --------------------- > Lab-Robotics Discussion > --------------------- > Can anyone tell me the impact of NOT making the radioactive counters Y2K > compliant. I have tried all sorts of things I cannot justify spending > $60k > to make few of them Y2K compliant. I see a problem if I use it for > calculting decay, but I never use it for that purpose. Any knowledge in > this regard is appreciated. > > Chakk > From: Julie Monagle Subject: 96/384 well plate washers with stackers Date: 22 Nov 99 14:20:56 -0500 Hello all, I would be interested to hear from users, any reccomendations for plate washers with stackers (96 & 384 compatible). Thanks in advance, Julie Monagle Neurogen Corporation RE: Glass 6-well plates Contact Fred Spike at Spike International Toll-free Phone: (800) 734-9408 fred@SPAMFOIL.spike.cc www.spike.cc He distributes a line of German made glass plates with different well patterns. Patrick Gaillard Oyster Bay Pump Works, Inc. ----- Original Message ----- From: Sam Pickering To: Lab-Robotics Discussion Mailing List Sent: Thursday, November 18, 1999 6:52 AM Subject: Lab-Robotics Discussion: Glass 6-well --------------------- Lab-Robotics Discussion --------------------- Does anybody know where I can get a glass 6-well plate from? I need to make some modifications to the inside of the wells, and then autoclave (121 degrees Centigrade, one bar guage). I don't want to have to gamma-irradiate, and all that I've found so far is polystyrene/ethylene/propylene/carbonate. Glass seems to only be used in the manufacture of 96-wells or more. Any help would be much appreciated, Sam Pickering. --- Lab-Robotics Discussion There is a web based companion to the LRIG Discussion Mailing List called DiscussWeb. The LRIG DiscussWeb allows for on-line messaging, responses, and reviewing of past posts. It may be found at https://www.lab-robotics.org/Web_Discuss/ From: Guard@SPAMFOIL.britbio.co.uk Subject: Lab-Robotics Discussion: Pipetting Cells into 96/384-well plates Date: Wed, 24 Nov 1999 09:32:28 +0000 I'm very interested to hear of peoples experiences with pipetting cell suspensions/seeding adherent cell lines into 96 and 384-well plates for the purpose of cell-based screening. Any information regarding choice of appropriate pipetting equipment and any cautionary measures to be taken whilst pipetting to plates would be greatly appreciated. Steve Guard D.Phil. Group Leader British Biotech Pharmaceuticals Ltd. Watlington Road Oxford OX33 1YA U.K. Tel: 44 (0)1865 748747 Fax: 44 (0)1865 781064 From: jfloeckher@SPAMFOIL.packardinstrument.com Subject: Re: Lab-Robotics Discussion: 96/384 well plate washers withstackers Date: Wed, 24 Nov 1999 07:55:37 -0500 Hello Julie, The CCS Packard division of Packard Instrument Company manufactures and sells a 96/384 well plate washer called the PlateWash, with an optional 50 plate stacker called the PlateStak. You can obtain more information about these products from the Packard web site by looking in http://www.packardinstrument.com. Best regards, Jim Floeckher To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: Jim Floeckher/PI/US/PBC) Person From/To: From: Julie Monagle Date: 11/22/99 07:20:56 PM GMT Subject: Lab-Robotics Discussion: 96/384 well plate washers with stackers --------------------- Lab-Robotics Discussion --------------------- Hello all, I would be interested to hear from users, any reccomendations for plate washers with stackers (96 & 384 compatible). Thanks in advance, Julie Monagle Neurogen Corporation --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: Jeff_Busnach@SPAMFOIL.Millipore.com Subject: Re: Lab-Robotics Discussion: 96/384 well plate washers withstackers Date: Wed, 24 Nov 1999 09:44:37 -0500 Julie, TOMTEC has the Quadra models with integrated stackers and 6 position decks. It can run a couple of stacks through the wash station and then restack the plates all automatically. www.tomtec.com Jeffrey S. Busnach MultiWell Products & Automation Scientist Email: Jeff_Busnach@SPAMFOIL.millipore.com Tel: U.S. (978) 762-5204 Fax: U.S. (978) 762-5384 MILLIPORE CORPORATION 17 Cherry Hill Drive Danvers, MA 01923 Julie Monagle on 11/22/99 02:20:56 PM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: Jeff Busnach/Bev/NA/Millipore) Subject: Lab-Robotics Discussion: 96/384 well plate washers with stackers --------------------- Lab-Robotics Discussion --------------------- Hello all, I would be interested to hear from users, any reccomendations for plate washers with stackers (96 & 384 compatible). Thanks in advance, Julie Monagle Neurogen Corporation --- Lab-Robotics Discussion To retrieve a help file on this list, send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just HELP as the subject. From: "Hare, Tim" Subject: service for barcoding of third party plates? Date: Wed, 24 Nov 1999 11:53:06 -0500 Anyone know of any companies that will barcode third-party microtiter plates? That is, I need to be able to send a batch of microtiter plates made by one vendor (that cannot barcode) to another vendor who will barcode the plates to our specification. Timothy R. Hare Research Biochemist Automated Biotechnology (952) Mail drop: NW-2 tim_hare@SPAMFOIL.merck.com Voice: (215) 652-3235 FAX: (215) 993-0182 From: martin.daffertshofer@SPAMFOIL.evotec.de Subject: Parallel Pipetting of micro-liter volumes Date: Wed, 24 Nov 1999 17:42:31 +0100 Does anybody gathered experiences of high throughput pipetting / dispensing in the micro liter domain. I want to use devices like Robbins Hydra or JOBI-Well 384/1536 to transfer (> 384 in parallel) less than a micro liter of Water/DMSO mixtures (0-100%) from plate to plate. Any information would be appreciated, Martin Daffertshofer. From: "Suzanne Martinez" Subject: Re: Lab-Robotics Discussion: 96/384 well plate washers withstackers Date: Wed, 24 Nov 1999 09:05:17 -0800 Julie, MDC does not have a 96/384 stacker yet(only a 96 well stacker), but I would be interested to find out what your application is and whether you think this is a typical trend in your area of science. Also, I'd be interested to know when you are in need of this washer. Thank you. Regards, Suzanne Martinez Skatron Product Manager Molecular Devices Corporation >>> Julie Monagle 11/22/99 11:20AM >>> --------------------- Lab-Robotics Discussion --------------------- Hello all, I would be interested to hear from users, any reccomendations for plate washers with stackers (96 & 384 compatible). Thanks in advance, Julie Monagle Neurogen Corporation --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: "Thomas J. Baiga" Subject: Laboratory Microwave Ovens for Synthesis Date: Wed, 24 Nov 1999 10:30:08 -0800 Our application group has been hearing a lot of positive comments about microwave assisted solid-phase synthesis. We're interested in developing some standard applications in this area which will also lead to the development of some new reaction block modules. I'd like to get some recommendations on which oven performs the best. Comments are more than welcome from folks using ovens outside of synthesis applications (i.e. acid digestion, sample prep, etc.). Ovens features of interest include: SAFETY, flexible design (for reaction vessels), and an ability to be automated or interfaced with robotics systems. Thanks in advance. Tom Baiga Charybdis Technologies From: "Gunnet, Joseph [PRI]" Subject: RE: Lab-Robotics Discussion: Pipetting Cells into 96/384-well plates Date: Wed, 24 Nov 1999 14:18:37 -0500 We've had alot of success using the ICN Titertek Multidrop/stacker in seeding cells into 96 well plates for HTS. The unit fits in a 6 foot cell culture hood with enough extra room to open and stack the plates. We've used the Multidrop for several different cell lines and have seen low variability within and between plates. With antibiotics and with good sterile technique we seldom if ever see contamination. Joe Gunnet The R.W. Johnson Pharm. Res. Institute Rt. 202 Ratitan, NJ 08869 (908) 704-4286 jgunnet@SPAMFOIL.prius.jnj.com > -----Original Message----- > From: Guard@SPAMFOIL.britbio.co.uk [SMTP:Guard@SPAMFOIL.britbio.co.uk] > Sent: Wednesday, November 24, 1999 4:32 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: Pipetting Cells into 96/384-well > plates > > --------------------- > Lab-Robotics Discussion > --------------------- > I'm very interested to hear of peoples experiences with pipetting cell > suspensions/seeding adherent cell lines into 96 and 384-well plates for > the > purpose of cell-based screening. > > Any information regarding choice of appropriate pipetting equipment and > any > cautionary measures to be taken whilst pipetting to plates would be > greatly > appreciated. > > Steve Guard D.Phil. > Group Leader > British Biotech Pharmaceuticals Ltd. > Watlington Road > Oxford OX33 1YA > U.K. > Tel: 44 (0)1865 748747 > Fax: 44 (0)1865 781064 > From: Jackbonham@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: Pipetting Cells into 96/384-well plates Date: Fri, 26 Nov 1999 20:59:01 EST In a message dated 11/26/99 2:28:12 PM Eastern Standard Time, JGUNNET@SPAMFOIL.prius.jnj.com writes: << We've had alot of success using the ICN Titertek Multidrop/stacker in seeding cells into 96 well plates for HTS. >> Just so there is no confusion...ICN Pharmaceuticals is no longer associated with Titertek and any inquiries to them regarding the Multidrop or Multidrop Stacker will be met with the equivalent of a dumb look. I would refer you to the number below. Jack Bonham Titertek Instruments, Inc. 256-859-8600 From: "Piet Arnaut-P" Subject: 24-well plate stirring Date: Fri, 26 Nov 1999 21:31:15 -0500 Hello, I am looking for a device that can apply mechanical stirring or ideally circular rubbing in 24-well plates. The device should come with a RPM control. Piet Arnaut Senior Research Associate Procter & Gamble ETC Temselaan 100 1853 Strombeek-Bever Belgium From: "jcelani" Subject: Re: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes Date: Sun, 28 Nov 1999 12:53:46 -0800 Apricot Designs makes dispensing units which have small footprints. They have a 384 well head which can do up to 1536. They can be found on the web and are made in Monrovia, CA. Their head engineer can address the limits for less than a ul. ph (626) 256-6088 John Celani -----Original Message----- From: martin.daffertshofer@SPAMFOIL.evotec.de To: Lab-Robotics Discussion Mailing List Date: Wednesday, November 24, 1999 2:59 PM Subject: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes >--------------------- >Lab-Robotics Discussion >--------------------- > > >Does anybody gathered experiences of high throughput pipetting / dispensing >in the micro liter domain. >I want to use devices like Robbins Hydra or JOBI-Well 384/1536 to transfer >(> 384 in parallel) less >than a micro liter of Water/DMSO mixtures (0-100%) from plate to plate. > >Any information would be appreciated, > >Martin Daffertshofer. > From: David Subject: Re: Lab-Robotics Discussion: 24-well plate stirring Date: Sun, 28 Nov 1999 23:44:59 +0000 Piet Arnaut-P wrote: > > --------------------- > Lab-Robotics Discussion > --------------------- > Hello, > I am looking for a device that can apply mechanical stirring or ideally circular > rubbing in 24-well plates. The device should come with a RPM control. > > Piet Arnaut > Senior Research Associate > Procter & Gamble ETC > Temselaan 100 > 1853 Strombeek-Bever > Belgium Our company often designs custom products. We would be interested in quoting if you can't find what you're looking for. -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com Just in: I have just received information from a generally reliable source deep within NASA that the latest failure of the main computer onboard MIR occurred when Russian cosmonauts attempted to delete Microsoft's Internet Explorer. From: "Steven R Cotterman" Subject: Re: Lab-Robotics Discussion: service for barcoding of third partyplates? Date: Sun, 28 Nov 1999 18:54:31 -0800 Tim, Beckman Coulter has an excellent print and apply module. It will apply barcodes on all four sides of standard and deep well plates. An integrated MS-710 bar code scanner is part of the standard system. If you would like more information let me know. Regards, Steve Cotterman Beckman Coulter 1-800-392-2164 x 9006 From: jfloeckher@SPAMFOIL.packardinstrument.com Subject: Reply to Ramesha, Chakk re: Y2K Date: Mon, 29 Nov 1999 07:59:37 -0500 The Packard Instrument Company Web site, at http://www.packardinstrument.com/index.htm, contains comprehensive information about the Y2K issue with its instruments. Included is information about what you can expect from Packard radioisotope counters that have not been updated to Y2K compliance. Jim Floeckher From: "BODE, Donald C." Subject: RE: Lab-Robotics Discussion: service for barcoding of third partyplates? Date: Mon, 29 Nov 1999 09:00:54 -0500 Becton-Dickinson (contact Stuart Gibb 201-847-5072) and Corning Costar (contact Lauri Cavison 800-492-1119 x8024) have the capability for high-volume barcoding of their own plates, but I don't know if they would do third-party plates. Chris Bode RPR > ---------- > From: Hare, Tim[SMTP:tim_hare@SPAMFOIL.merck.com] > Sent: Wednesday, November 24, 1999 11:53 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: service for barcoding of third > party plates? > > --------------------- > Lab-Robotics Discussion > --------------------- > Anyone know of any companies that will barcode third-party microtiter > plates? > That is, I need to be able to send a batch of microtiter plates made by > one > vendor (that cannot barcode) to another vendor who will barcode the plates > to our specification. > > Timothy R. Hare > Research Biochemist > Automated Biotechnology (952) > Mail drop: NW-2 > tim_hare@SPAMFOIL.merck.com > Voice: (215) 652-3235 > FAX: (215) 993-0182 > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > From: "BODE, Donald C." Subject: RE: Lab-Robotics Discussion: Pipetting Cells into 96/384-well plates Date: Mon, 29 Nov 1999 09:12:36 -0500 We use the Labsystems Multidrop with their version of the Zymark Twister (80-plate capacity) for dispensing cell suspensions into 96-well plates on the benchtop (non-sterile environment). The Multidrop is easy, flexible, fast and pretty reliable. With antibiotics, we and others have found that there is no problem with contamination of mammalian cell cultures for a one- to two-day assay protocol under these conditions. We have not done any cell-based assays in 384-well plates yet. Chris > ---------- > From: Guard@SPAMFOIL.britbio.co.uk[SMTP:Guard@SPAMFOIL.britbio.co.uk] > Sent: Wednesday, November 24, 1999 4:32 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: Pipetting Cells into 96/384-well > plates > > --------------------- > Lab-Robotics Discussion > --------------------- > I'm very interested to hear of peoples experiences with pipetting cell > suspensions/seeding adherent cell lines into 96 and 384-well plates for > the > purpose of cell-based screening. > > Any information regarding choice of appropriate pipetting equipment and > any > cautionary measures to be taken whilst pipetting to plates would be > greatly > appreciated. > > Steve Guard D.Phil. > Group Leader > British Biotech Pharmaceuticals Ltd. > Watlington Road > Oxford OX33 1YA > U.K. > Tel: 44 (0)1865 748747 > Fax: 44 (0)1865 781064 > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > From: "Minor, Lisa [PRI]" Subject: RE: Lab-Robotics Discussion: Pipetting Cells into 96/384-well plates Date: Mon, 29 Nov 1999 09:21:53 -0500 As of November, the Multidrop also comes as a 96 well/384 well dispenser complete with stackers. Although I have had no experience with the 384 well dispenser in plating cells, I have recently had a demo and it looks promising. As Joe has said, contamination of cell cultures using the Multidrop has not been an issue as long as proper sterile techniques are implemented. Lisa Minor 908-704-4403 The R.W. Johnson Pharm. Res. Institute Rt. 202 Ratitan, NJ 08869 lminor@SPAMFOIL.prius.jnj.com > -----Original Message----- > From: Gunnet, Joseph [PRI] [SMTP:JGUNNET@SPAMFOIL.prius.jnj.com] > Sent: Wednesday, November 24, 1999 2:19 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: Pipetting Cells into 96/384-well > plates > > --------------------- > Lab-Robotics Discussion > --------------------- > We've had alot of success using the ICN Titertek Multidrop/stacker in > seeding cells into 96 well plates for HTS. The unit fits in a 6 foot cell > culture hood with enough extra room to open and stack the plates. We've > used > the Multidrop for several different cell lines and have seen low > variability > within and between plates. With antibiotics and with good sterile > technique > we seldom if ever see contamination. > > Joe Gunnet > The R.W. Johnson Pharm. Res. Institute > Rt. 202 > Ratitan, NJ 08869 > > (908) 704-4286 > jgunnet@SPAMFOIL.prius.jnj.com > > > -----Original Message----- > > From: Guard@SPAMFOIL.britbio.co.uk [SMTP:Guard@SPAMFOIL.britbio.co.uk] > > Sent: Wednesday, November 24, 1999 4:32 AM > > To: discussion@SPAMFOIL.lab-robotics.org > > Subject: Lab-Robotics Discussion: Pipetting Cells into 96/384-well > > plates > > > > --------------------- > > Lab-Robotics Discussion > > --------------------- > > I'm very interested to hear of peoples experiences with pipetting cell > > suspensions/seeding adherent cell lines into 96 and 384-well plates for > > the > > purpose of cell-based screening. > > > > Any information regarding choice of appropriate pipetting equipment and > > any > > cautionary measures to be taken whilst pipetting to plates would be > > greatly > > appreciated. > > > > Steve Guard D.Phil. > > Group Leader > > British Biotech Pharmaceuticals Ltd. > > Watlington Road > > Oxford OX33 1YA > > U.K. > > Tel: 44 (0)1865 748747 > > Fax: 44 (0)1865 781064 > > > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: "Smith, Todd, HMR/US" Subject: RE: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes Date: Mon, 29 Nov 1999 09:35:30 -0600 Hello John Celani. How are you? I am curious about this Apricot Design pipettor you posted about on the Lab Automation board. Is it the same one they displayed at lab automation last year? I recall that pipettor being a stand alone Hydra look alike that really wasn't suited to HTS automation. Any info you could forward would be appreciated. We are looking for a known 384 pipettor with 1536 capability that is HTS automation friendly. Its really too bad that CCS and Beckman had to screw up their relationship so bad that the CCS 384 head will never see a marriage with the Beckman Software or a Multimek. Most likely I'll see you in Palm Springs. Cheers, Todd Smith Biology / Chemistry Automation Selectide, a subsidiary of Hoechst Marion Roussel 1580 East Hanley Boulevard Tucson, Arizona 85737 (520) 544-5884 Phone (520) 575-1439Fax todd.smith@SPAMFOIL.hmrag.com -----Original Message----- From: jcelani [mailto:jcelani@SPAMFOIL.ix.netcom.com] Sent: Sunday, November 28, 1999 1:54 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes --------------------- Lab-Robotics Discussion --------------------- Apricot Designs makes dispensing units which have small footprints. They have a 384 well head which can do up to 1536. They can be found on the web and are made in Monrovia, CA. Their head engineer can address the limits for less than a ul. ph (626) 256-6088 John Celani -----Original Message----- From: martin.daffertshofer@SPAMFOIL.evotec.de To: Lab-Robotics Discussion Mailing List Date: Wednesday, November 24, 1999 2:59 PM Subject: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes >--------------------- >Lab-Robotics Discussion >--------------------- > > >Does anybody gathered experiences of high throughput pipetting / dispensing >in the micro liter domain. >I want to use devices like Robbins Hydra or JOBI-Well 384/1536 to transfer >(> 384 in parallel) less >than a micro liter of Water/DMSO mixtures (0-100%) from plate to plate. > >Any information would be appreciated, > >Martin Daffertshofer. > From: Julie Monagle Subject: RE: Lab-Robotics Discussion: 96/384 well plate washers withstack Date: 30 Nov 99 12:11:04 -0500 Hi Suzanne, Thanks for the info. I just got off the phone with Sarah Bliven. She is faxing me a quote for the 96 skan stacker. The reason for my request of 96 & 384 with stacking is that most of what we do is in 96 wells currently, but we would like the ability to investigate 384 without having to purchase another washer until we know that it will work for us. The ability to have stacking with both 96 & 384 is a nice option, because once a user gets used to the stackers they don't want to be tied to the washer in order to have a 384 well assay. At this point it looks like we will have to work with a 96 with stackers and invest in a 384 when the time is ready. Thanks, Julie Monagle Neurogen Corporation Suzanne Martinez wrote: >--------------------- >Lab-Robotics Discussion >--------------------- >Julie, >MDC does not have a 96/384 stacker yet(only a 96 well stacker), but I would be >interested to find out what your application is and whether you think this >is a typical trend in your area of science. Also, I'd be interested to know when >you are in need of this washer. >Thank you. > > >Regards, > >Suzanne Martinez >Skatron Product Manager >Molecular Devices Corporation > > >>>> Julie Monagle 11/22/99 11:20AM >>> >--------------------- >Lab-Robotics Discussion >--------------------- >Hello all, > >I would be interested to hear from users, any reccomendations for plate >washers with stackers (96 & 384 compatible). > >Thanks in advance, > > >Julie Monagle >Neurogen Corporation > From: "ANUJA.ANILKUMAR.SONALKER" Subject: pursuing my undergrads Date: Thu, 2 Dec 1999 08:22:08 +0000 (IST) This is an important query! In fact one of the major reasons for joining lab-robotics. I am pursuing my undergrads in Instrumentation/Control & want to go in for a further MS. So far I'm thinking of Intelligent Systems/Robotics/Control Syatems. Can anyone here highlight the scope of these subjects in the near future or are they just dead subjects. My second option is Telecommunications/Signal Processing. Please help ANUJA (A confused student) From: "Thomas J. Baiga" Subject: Re: Lab-Robotics Discussion: pursuing my undergrads Date: Wed, 01 Dec 1999 23:45:33 -0800 I am pursuing my undergrads in Instrumentation/Control & want to go in for a further MS. So far I'm thinking of Intelligent Systems/Robotics/Control Systems. Can anyone here highlight the scope of these subjects in the near future or are they just dead subjects. My second option is Telecommunications/Signal Processing. Please help ANUJA (A confused student) Anuja, Definitely not dead subjects. In fact, I expect the market for cross-trained engineers to grow significantly in the next few years. The program that your looking for(based upon your interests in Intelligent Systems/Robotics/Control Systems) is called MECHATRONICS. This is an interdisciplinary program combining research in smart materials, robotics, intelligent systems, and control systems. Rensselaer Polytechnic Institute (Troy, NY) created one of the first programs of this kind. Please refer to http://www.eng.rpi.edu/ and look at Professor Craig's research. Good luck, and when you finish your MS, please send your CV to our HR Dept., we're always looking for talented engineers. Tom ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ Hello Todd, I sense that there maybe a story behind some of your comments. Could you mention your hesitation in regards to the CCS Packard 384 tip device. Recently, I have been talking with CCS Packard and I am very interested in learning all that I can about this device. Further, any comments that you might have regarding the MiniTrak would be interesting to hear. Jim -----Original Message----- From: Smith, Todd, HMR/US [mailto:Todd.Smith@SPAMFOIL.hmrag.com] Sent: Monday, November 29, 1999 10:36 AM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes --------------------- Lab-Robotics Discussion --------------------- Hello John Celani. How are you? I am curious about this Apricot Design pipettor you posted about on the Lab Automation board. Is it the same one they displayed at lab automation last year? I recall that pipettor being a stand alone Hydra look alike that really wasn't suited to HTS automation. Any info you could forward would be appreciated. We are looking for a known 384 pipettor with 1536 capability that is HTS automation friendly. Its really too bad that CCS and Beckman had to screw up their relationship so bad that the CCS 384 head will never see a marriage with the Beckman Software or a Multimek. Most likely I'll see you in Palm Springs. Cheers, Todd Smith Biology / Chemistry Automation Selectide, a subsidiary of Hoechst Marion Roussel 1580 East Hanley Boulevard Tucson, Arizona 85737 (520) 544-5884 Phone (520) 575-1439Fax todd.smith@SPAMFOIL.hmrag.com -----Original Message----- From: jcelani [mailto:jcelani@SPAMFOIL.ix.netcom.com] Sent: Sunday, November 28, 1999 1:54 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes --------------------- Lab-Robotics Discussion --------------------- Apricot Designs makes dispensing units which have small footprints. They have a 384 well head which can do up to 1536. They can be found on the web and are made in Monrovia, CA. Their head engineer can address the limits for less than a ul. ph (626) 256-6088 John Celani -----Original Message----- From: martin.daffertshofer@SPAMFOIL.evotec.de To: Lab-Robotics Discussion Mailing List Date: Wednesday, November 24, 1999 2:59 PM Subject: Lab-Robotics Discussion: Parallel Pipetting of micro-liter volumes >--------------------- >Lab-Robotics Discussion >--------------------- > > >Does anybody gathered experiences of high throughput pipetting / dispensing >in the micro liter domain. >I want to use devices like Robbins Hydra or JOBI-Well 384/1536 to transfer >(> 384 in parallel) less >than a micro liter of Water/DMSO mixtures (0-100%) from plate to plate. > >Any information would be appreciated, > >Martin Daffertshofer. >