This is a multi-part MIME document. --=_X9040113073042346Y= Content-Type: text/plain From: "Daly, Maureen" Subject: GeneVac Maintenance Date: Mon, 1 Mar 1999 15:08:12 -0500 Over the past month, I have read the various messages discussing the GeneVac. My question is regarding maintenance on the GeneVac. The User Manuals are not very descriptive in this area. Therefore, I am wondering what maintenance schedules people have developed and/or follow. Please let me know. Thanks! Maureen Daly Chemical Process Engineer ArQule Inc. --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: Henry M Holava Subject: Re: Lab-Robotics: Automated plate unsealing Date: Mon, 01 Mar 1999 16:21:37 -0500 I'm looking into automated plate sealing with the mylar backed foil. We would not unseal but pierce the seal for liquid transfer and then discard the pierced plate. If anyone has experience with this type of seal I would be interested in how well the mylar hold up with DMSO and how well the seal it self hold up under various temperature storage conditions. Henry Holava Victor Holland wrote: > > --------------------- > Lab-Robotics > --------------------- > It seems that there are several options for automated sealing of > microplates. Is there any automated system that can unseal > plates? If not, how do the automated systems deal with the > sealed plates? Are they taken off line for manual unsealing? > Do some automated systems pierce or otherwise penetrate the > seal? If so, how is the plate re-sealed? --=_X9040113073042346Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: Andy Zaayenga Subject: Re: Lab-Robotics: Automated plate unsealing Date: Mon, 01 Mar 1999 20:43:03 -0500 Henry, TekCel Corporation just announced today the first microplate cover capable of fully automated sealing and unsealing! The SealTite system eliminates adhesives, bubble mats, and loose covers. The patented SealTite plate cover consists of a thin Teflon seal that covers the wells of the microplate. On top of the Teflon seal is a soft Neoprene material that applies an even force to the Teflon covering the wells. Finally there is a plastic cover that is held on top of the plate by spring loaded straps that run underneath the microplate. To install a cover, SealTite is picked up by the robot and moved into position so that the springs can be deflected down. The plate is slid under the cover. The springs are then released, pulling the cover onto the plate. The sequence is reversed to remove the cover. * SealTite offers an inert and gas tight seal to the chemicals for long term storage. * SealTite is reusable and can be repeatedly installed and removed in a fully automated fashion. Payback may be achieved in as little as seven seal/unseal cycles. * SealTite covered plates may be uncovered moments before use and recovered immediately after use. * Worker safety is greatly enhanced. * Cross contamination from peeling off adhesive seals is eliminated. * SealTite is an universal solution fitting shallow microplates, halfwells, deepwells, cubetubes, and minitibes. Combined with the TekCel PlateServer, any automated system on the market today may be serviced with SealTite covered plates. Our first adopter of this exciting new technology plans to store their entire compound library with SealTite covers and service it with a PlateServer linked to an Assay TekBench. For more detailed information please visit our SealTite web page: http://tekcel.com/sealtite.htm Andy Zaayenga TekCel Corporation Mailto:andy.zaayenga@SPAMFOIL.tekcel.com At 04:21 PM 3/1/99 -0500, Henry M Holava wrote to Andy: |--------------------- |Lab-Robotics |--------------------- |I'm looking into automated plate sealing with the mylar backed foil. We |would not unseal but pierce the seal for liquid transfer and then |discard the pierced plate. If anyone has experience with this type of |seal I would be interested in how well the mylar hold up with DMSO and |how well the seal it self hold up under various temperature storage |conditions. | |Henry Holava | |Victor Holland wrote: |> |> --------------------- |> Lab-Robotics |> --------------------- |> It seems that there are several options for automated sealing of |> microplates. Is there any automated system that can unseal |> plates? If not, how do the automated systems deal with the |> sealed plates? Are they taken off line for manual unsealing? |> Do some automated systems pierce or otherwise penetrate the |> seal? If so, how is the plate re-sealed? | | --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: Paul Day Subject: Re: Lab-Robotics: Automated plate unsealing Date: Tue, 02 Mar 1999 12:32:11 -0800 Henry M Holava wrote: > > --------------------- > Lab-Robotics > --------------------- > I'm looking into automated plate sealing with the mylar backed foil. We > would not unseal but pierce the seal for liquid transfer and then > discard the pierced plate. If anyone has experience with this type of > seal I would be interested in how well the mylar hold up with DMSO and > how well the seal it self hold up under various temperature storage > conditions. > > Henry Holava > > Victor Holland wrote: > > > > --------------------- > > Lab-Robotics > > --------------------- > > It seems that there are several options for automated sealing of > > microplates. Is there any automated system that can unseal > > plates? If not, how do the automated systems deal with the > > sealed plates? Are they taken off line for manual unsealing? > > Do some automated systems pierce or otherwise penetrate the > > seal? If so, how is the plate re-sealed? You may want to consider our Auto Laboratory Plate Sealer (ALPS) system which is based on heat sealing technology and has been integrated into robotic systems. The ALPS is compatable with a variety of different heat sealing foils and and films, one of which is a polypopylene(PP) piercable foil laminate which provides a very strong heat sealed bond. The seal is extremely effective and will eliminate any leakage or evaporation even at high/low temperatures. It also has very good solvent resistance because it is a PP laminate and will withstand DMSO. The Easy Pierce foil is available on both a roll (cat no. AB-0798) for use with the ALPS or as cut sheets AB-0813 for use with our manual sealer. Our products are distributed in the US by Marsh Biomedical tel: 800-445-2812. Paul Day Advanced Biotechnologies Phone +44(0)1372 723456 Fax +44(0)1372 741414 Web Site http://www.adbio.co.uk --=_X9040113073042346Y= Content-Type: text/plain From: "Smith, David" Subject: RE: Lab-Robotics: GeneVac Maintenance Date: Tue, 2 Mar 1999 10:45:16 -0500 GeneVac basic maintenance if fairly straight forward. We empty the condenser usually every day or after each use. Checking the oil in the vacuum pump is done once a week. If needed, the oil is topped off. We clean the evaporation chamber as needed (once a month or so) with acetone and use a thin film of high vacuum grease on the O-ring. Once a year, a thorough cleaning of the valves is done. I hope this helps. David Smith Rhone-Poulenc Ag. Co. Discovery Chemistry 2 TW Alexander Dr. RTP, NC 27709 Phone: (919) 549-2966 Fax: (919) 549-0054 > ---------- > From: Daly, Maureen[SMTP:MDaly@SPAMFOIL.arqule.com] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Monday, March 01, 1999 3:08 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: GeneVac Maintenance > > --------------------- > Lab-Robotics > --------------------- > Over the past month, I have read the various messages discussing the > GeneVac. My question is regarding maintenance on the GeneVac. The > User > Manuals are not very descriptive in this area. Therefore, I am > wondering what maintenance schedules people have developed and/or > follow. Please let me know. Thanks! > > Maureen Daly > Chemical Process Engineer > ArQule Inc. > --=_X9040113073042346Y= Content-Type: text/plain From: "Li, Chuck" Subject: mixing magnetic beads Date: Wed, 3 Mar 1999 17:20:08 -0800 Does anyone have any suggestions for mixing magnetic bead solution in a reservoir which has a footprint of a 96 well microtiter plate? This reservoir is to be used with a Multimek for bulk bead solution dispensing. Thanks for your inputs. Chuck --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Raymond Reilly" Subject: Re: Lab-Robotics: mixing magnetic beads Date: Thu, 4 Mar 1999 08:47:57 -0500 Hi Chuck, This doesn't answer your specific question but CRS Automated Solutions has a standard DNA Purification Workstation which includes a bead mixing and dispensing station using a standard MTP format. If you'd like more information about it please contact me at RFReilly@SPAMFOIL.worldnet.att.net or (908)665-7757. Regards, Ray Reilly -----Original Message----- From: Li, Chuck To: Lab-Robotics Mailing List Date: Thursday, March 04, 1999 12:34 AM Subject: Lab-Robotics: mixing magnetic beads >--------------------- >Lab-Robotics >--------------------- >Does anyone have any suggestions for mixing magnetic bead solution in a >reservoir which has a footprint of a 96 well microtiter plate? This >reservoir is to be used with a Multimek for bulk bead solution dispensing. > >Thanks for your inputs. > > >Chuck > > --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: Andy Zaayenga Subject: Re: Lab-Robotics: mixing magnetic beads Date: Fri, 05 Mar 1999 00:00:43 -0500 Chuck, Take a look at http://www.hypertask.com/mixer.htm. Justin Webster makes this station for the Biomek and it is adaptable to the Multimek. Andy At 05:20 PM 3/3/99 -0800, Li, Chuck wrote to Andy: |--------------------- |Lab-Robotics |--------------------- |Does anyone have any suggestions for mixing magnetic bead solution in a |reservoir which has a footprint of a 96 well microtiter plate? This |reservoir is to be used with a Multimek for bulk bead solution dispensing. | |Thanks for your inputs. | | |Chuck | | --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" From: "Werner, Chris" Subject: RE: Lab-Robotics: mixing magnetic beads Date: Fri, 5 Mar 1999 07:47:00 -0500 I'd have a look at V&P Scientific stirring devices. They look pretty good. They are at : http://www.vp-scientific.com/ Hope this helps. Chris Werner Group Leader, Engineering ArQule, Inc. -----Original Message----- From: Li, Chuck [SMTP:chuckl@SPAMFOIL.amgen.com] Sent: Wednesday, March 03, 1999 8:20 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: mixing magnetic beads --------------------- Lab-Robotics --------------------- Does anyone have any suggestions for mixing magnetic bead solution in a reservoir which has a footprint of a 96 well microtiter plate? This reservoir is to be used with a Multimek for bulk bead solution dispensing. Thanks for your inputs. Chuck --=_X9040113073042346Y= Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 7bit From: "Laskody, Becky C" Subject: Sonicating cleaner for Genesis fixed tips Date: Mon, 8 Mar 1999 11:46:03 -0500 Hi Folks, I've heard of people using a sonicating bath to help clean fixed pipet tips, such as Genesis or Multiprobe, in place. For example, clean tips between transfers of SPA beads at a wash station outfitted with a sonicator. Any one know of a manufacturer for such a sonicating bath (very slim footprint to minimize deck space usage)? Also, an optical sensor to turn it on and off as the tips approach would be desirable. Thanks for your help, Becky Laskody * --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: dunlapr@SPAMFOIL.kodak.com Subject: Re: Lab-Robotics: Sonicating cleaner for Genesis fixed tips Date: Mon, 8 Mar 1999 15:07:51 -0500 From: Richard P. Dunlap I use an ultrasonic pen cleaner from Koh-I-Noor, model 25K42. Their web site is, www.koh-i-norr.com --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: Dominic Joseph Subject: Re: Lab-Robotics: Sonicating cleaner for Genesis fixed tips Date: Mon, 08 Mar 1999 16:28:36 -0500 www.koh-i-noor.com connects to the site At 03:07 PM 3/8/99 -0500, you wrote: >--------------------- >Lab-Robotics >--------------------- > > >From: Richard P. Dunlap > >I use an ultrasonic pen cleaner from Koh-I-Noor, model 25K42. Their web >site is, www.koh-i-norr.com > > > Dominic Joseph ------------------------------------------------------------------- Millennium Pharmaceuticals | Email: joseph@SPAMFOIL.mpi.com | 640 Memorial Drive | Phone: (617) 679-7473 | Cambridge, MA 02139 | Fax: (617) 621-3520 | ------------------------------------------------------------------- --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: David Meyer Subject: reader/imager Date: Thu, 11 Mar 1999 16:05:46 -0500 Does anyone know of a high quality plate reader/imager, something similar to the Bio-Rad Ultramark but images faster and with better resolution?? DAVE ________________________________________ David F. Meyer, Ph.D. Scientist Millennium Pharmaceuticals Inc. 40 Erie St. Cambridge, MA 02139 Tel: (617) 679 7475 Fax: (617) 374 7790 email: dmeyer@SPAMFOIL.mpi.com www.mlnm.com _______________________________________ --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: ifeygin@SPAMFOIL.pharmacop.com (Feygin, Ilya) Subject: Re: Lab-Robotics: reader/imager Date: Fri, 12 Mar 1999 08:28:19 -0500 Dave, Did you have a chance to talk to Dr. Greg Kirk at Millenium? Ilya Feygin Pharmacopeia At 04:05 PM 3/11/99 -0500, you wrote: >--------------------- >Lab-Robotics >--------------------- >Does anyone know of a high quality plate reader/imager, something similar >to the Bio-Rad Ultramark but images faster and with better resolution?? > >DAVE > > > >________________________________________ > >David F. Meyer, Ph.D. >Scientist >Millennium Pharmaceuticals Inc. >40 Erie St. >Cambridge, MA 02139 > >Tel: (617) 679 7475 >Fax: (617) 374 7790 >email: dmeyer@SPAMFOIL.mpi.com >www.mlnm.com > >_______________________________________ --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" From: Susan Vigilante Subject: RE: Lab-Robotics: reader/imager Date: Fri, 12 Mar 1999 07:24:14 -0800 Check out Molecular Devices. They have a great reader, but more importantly they have awesome software. I used to work for them and still think they have the best. I am with Argonaut now, so if you need any organic synthesizers let me know!! Susan Vigilante -----Original Message----- From: David Meyer [mailto:dmeyer@SPAMFOIL.mpi.com] Sent: Thursday, March 11, 1999 1:06 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: reader/imager --------------------- Lab-Robotics --------------------- Does anyone know of a high quality plate reader/imager, something similar to the Bio-Rad Ultramark but images faster and with better resolution?? DAVE ________________________________________ David F. Meyer, Ph.D. Scientist Millennium Pharmaceuticals Inc. 40 Erie St. Cambridge, MA 02139 Tel: (617) 679 7475 Fax: (617) 374 7790 email: dmeyer@SPAMFOIL.mpi.com www.mlnm.com _______________________________________ --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: "Ray Boutin" Subject: Re: Lab-Robotics: reader/imager Date: Sat, 13 Mar 1999 10:08:34 -0500 Dave, Anthos makes a couple of durable plate readers that can also image a well (35 measurements across the diameter). Measurement speed is about 5 sec. Ray Boutin Rosys Anthos At 04:05 PM 3/11/99 -0500, you wrote: >--------------------- >Lab-Robotics >--------------------- >Does anyone know of a high quality plate reader/imager, something similar >to the Bio-Rad Ultramark but images faster and with better resolution?? > >DAVE > > > >________________________________________ > >David F. Meyer, Ph.D. >Scientist >Millennium Pharmaceuticals Inc. >40 Erie St. >Cambridge, MA 02139 > >Tel: (617) 679 7475 >Fax: (617) 374 7790 >email: dmeyer@SPAMFOIL.mpi.com >www.mlnm.com > >_______________________________________ > > --=_X9040113073042346Y= Content-Type: text/plain From: "Daly, Maureen" Subject: GeneVac Atlas or Mega Date: Mon, 15 Mar 1999 08:55:09 -0500 I am wondering if anyone has had any issues (i.e.: failures, etc.) with the rotor assembly on the GeneVac, which may be resultant of heavy loads and/or imbalancing. Any information would be greatly appreciated. Thank you! Maureen K. Daly Chemical Process Engineer ArQule, Inc. --=_X9040113073042346Y= Content-Type: text/plain From: "Smith, David" Subject: RE: Lab-Robotics: GeneVac Atlas or Mega Date: Mon, 15 Mar 1999 13:05:38 -0500 Maureen, Our GeneVac Atlas is 3 years old and we have not had a problem with the rotor assembly. Indeed, we have used weights of up to 800g on each swing of the Atlas and there has been no deformation. We do pay attention balancing. Each tube in each rack has approximately the same amount of solvent. Our Mega is new, just 1 month old but we have not seen a problem with it either. David Smith Rhone-Poulenc Ag. Co. Discovery Chemistry 2 TW Alexander Dr. RTP, NC 27709 Phone: (919) 549-2966 Fax: (919) 549-0054 > ---------- > From: Daly, Maureen[SMTP:MDaly@SPAMFOIL.arqule.com] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Monday, March 15, 1999 8:55 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: GeneVac Atlas or Mega > > --------------------- > Lab-Robotics > --------------------- > I am wondering if anyone has had any issues (i.e.: failures, etc.) > with the > rotor assembly on the GeneVac, which may be resultant of heavy loads > and/or > imbalancing. Any information would be greatly appreciated. > > Thank you! > > Maureen K. Daly > Chemical Process Engineer > ArQule, Inc. > --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: "Votaw, Greg " Subject: RE: Lab-Robotics: GeneVac Atlas or Mega Date: Tue, 16 Mar 1999 15:04:38 -0500 I'm in the process of sending our HT-4 back to England for repair because of rotor problems. Genevac says it is damaged because it was dropped during shipment. It was fixed once before at our site now they are sending it to England for a rebuild. I assumed GeneVac is correct with the "dropped in shipment" theory because we use very small samples and always balance the load. Gregory Votaw Gregory A. Votaw Diversity Sciences GV85829@SPAMFOIL.Glaxowellcome.com > -----Original Message----- > From: Smith, David [SMTP:DSmith@SPAMFOIL.rp-agro.com] > Sent: Monday, March 15, 1999 1:06 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: GeneVac Atlas or Mega > > --------------------- > Lab-Robotics > --------------------- > Maureen, > > Our GeneVac Atlas is 3 years old and we have not had a problem with the > rotor assembly. Indeed, we have used weights of up to 800g on each > swing of the Atlas and there has been no deformation. We do pay > attention balancing. Each tube in each rack has approximately the same > amount of solvent. > > Our Mega is new, just 1 month old but we have not seen a problem with it > either. > David Smith > Rhone-Poulenc Ag. Co. > Discovery Chemistry > 2 TW Alexander Dr. > RTP, NC 27709 > Phone: (919) 549-2966 > Fax: (919) 549-0054 > > > ---------- > > From: Daly, Maureen[SMTP:MDaly@SPAMFOIL.arqule.com] > > Reply To: discussion@SPAMFOIL.lab-robotics.org > > Sent: Monday, March 15, 1999 8:55 AM > > To: discussion@SPAMFOIL.lab-robotics.org > > Subject: Lab-Robotics: GeneVac Atlas or Mega > > > > --------------------- > > Lab-Robotics > > --------------------- > > I am wondering if anyone has had any issues (i.e.: failures, etc.) > > with the > > rotor assembly on the GeneVac, which may be resultant of heavy loads > > and/or > > imbalancing. Any information would be greatly appreciated. > > > > Thank you! > > > > Maureen K. Daly > > Chemical Process Engineer > > ArQule, Inc. > > > --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: Jeff_Busnach@SPAMFOIL.Millipore.com Subject: Flourescence Readers Date: Wed, 17 Mar 1999 11:01:51 -0500 We are trying to decide between a Tecan SpectraFlour PLUS and a SpectraMAX Gemini for flourescence readers. Can anyone offer some opinions/experience with these two instruments? In particular, we are concerned with bulb life, service intervals, validation service and expense, support, reliability, and performance. Our requirement is for a Q.C. instrument that can do both 96 and 384 well Top read and Bottom read Flourescence. Are there any other players on the market in the under 30K price range? Thanks for your help! Jeff Busnach Automation and Multiwell Products Specialist Millipore Corporation 17 Cherry Hill Drive Danvers, MA 1-800-426-4266 x5204 --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: Brendan_J_Connolly@SPAMFOIL.sbphrd.com Subject: Print and apply bar-code instrumentation Date: Wed, 17 Mar 1999 17:07:01 +0000 We are looking at implementing a system to print and apply bar codes to microtiter plates and would like any information on instrumentation that can perform this task. This includes the plate capacity (Stackers) How long it takes to label 100 plates How the bar-codes are loaded into the printer (manual or file - if so what format is the file in) Cost of a system Reliability of a system Thanks Brendan --=_X9040113073042346Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7BIT From: "Gert-Jan Euverink" Subject: Re: Lab-Robotics: Flourescence Readers Date: Thu, 18 Mar 1999 06:20:54 +0100 BMG-labarotories makes a nice robot friendly fluorescense reader (Fluorstar) http://www.bmg-labtechnologies.com/ However, this reader uses two filter wheels whereas the spectramax uses two monochromaters. So fluorescense spectra can be recorded (if support is included in the software) > --------------------- > Lab-Robotics > --------------------- > > > We are trying to decide between a Tecan SpectraFlour PLUS and > a SpectraMAX Gemini for flourescence readers. > > Can anyone offer some opinions/experience with these two instruments? > > In particular, we are concerned with bulb life, service intervals, > validation service and expense, > support, reliability, and performance. > > Our requirement is for a Q.C. instrument that can do both 96 and 384 well > Top read and Bottom read Flourescence. > Are there any other players on the market in the under 30K price range? > > Thanks for your help! > > Jeff Busnach > Automation and Multiwell Products Specialist > Millipore Corporation > 17 Cherry Hill Drive > Danvers, MA > 1-800-426-4266 x5204 > +++------------------------------------------------------------+++ Dr. G.J.W. Euverink BioExplore, High throughput Screening Facility, Groningen Biomolecular Sciences and Biotechnological Institute Kerklaan 30, 9751 NN Haren, The Netherlands phone: +31-(0)50-3632166/7633 fax: +31-(0)50-3632154 http://www.biol.rug.nl/bioexplore/bioexplore.html (under construction) ---++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++--- --=_X9040113073042346Y= Content-Type: text/plain;charset="Windows-1252" Content-Transfer-Encoding: 7bit From: "Dr. Martin Daffertshofer" Subject: 384-well plates with glass bottom Date: Thu, 18 Mar 1999 09:26:16 +0100 Does anyone know of a manufacturer of 384-well plates with glass bottom? Thanks Martin Daffertshofer EVOTEC BioSystems --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" From: Dan Murphy Subject: RE: Lab-Robotics: Print and apply bar-code instrumentation Date: Thu, 18 Mar 1999 08:46:20 -0500 Check out the Zymark Presto Labeler, it interfaces to the Twister Plate Stacker which can hold up to 80 plates. There are three options for entering the barcodes(manual, a template that is numerically incremented or a comma delimited ascii file. Once it is setup and running the Twister labeler combo has performed very well for our customers, I'm not sure of the cost but for further info check out http://www.zymark.com/MARKETIN/PRODUCTS/LABELER/Labeler.htm Thanks, Dan Murphy Zymark Corporation -----Original Message----- From: Brendan_J_Connolly@SPAMFOIL.sbphrd.com [mailto:Brendan_J_Connolly@SPAMFOIL.sbphrd.com] Sent: Wednesday, March 17, 1999 12:07 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Print and apply bar-code instrumentation --------------------- Lab-Robotics --------------------- We are looking at implementing a system to print and apply bar codes to microtiter plates and would like any information on instrumentation that can perform this task. This includes the plate capacity (Stackers) How long it takes to label 100 plates How the bar-codes are loaded into the printer (manual or file - if so what format is the file in) Cost of a system Reliability of a system Thanks Brendan --=_X9040113073042346Y= Content-Type: text/plain From: "Sheldon, Adrian" Subject: RE: Lab-Robotics: Flourescence Readers Date: Thu, 18 Mar 1999 09:38:38 -0500 We have BMG fluorescence detectors. The hardware is good, and they can read 384 well plates, but our experience with the reliability and ease-of-use of the software has been disappointing. > ---------- > From: Gert-Jan Euverink[SMTP:G.J.W.Euverink@SPAMFOIL.biol.rug.nl] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Thursday, March 18, 1999 12:20 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Flourescence Readers > > --------------------- > Lab-Robotics > --------------------- > > BMG-labarotories makes a nice robot friendly fluorescense reader > (Fluorstar) http://www.bmg-labtechnologies.com/ > > However, this reader uses two filter wheels whereas the > spectramax uses two monochromaters. So fluorescense spectra > can be recorded (if support is included in the software) > > > --------------------- > > Lab-Robotics > > --------------------- > > > > > > We are trying to decide between a Tecan SpectraFlour PLUS and > > a SpectraMAX Gemini for flourescence readers. > > > > Can anyone offer some opinions/experience with these two instruments? > > > > In particular, we are concerned with bulb life, service intervals, > > validation service and expense, > > support, reliability, and performance. > > > > Our requirement is for a Q.C. instrument that can do both 96 and 384 > well > > Top read and Bottom read Flourescence. > > Are there any other players on the market in the under 30K price range? > > > > Thanks for your help! > > > > Jeff Busnach > > Automation and Multiwell Products Specialist > > Millipore Corporation > > 17 Cherry Hill Drive > > Danvers, MA > > 1-800-426-4266 x5204 > > > > > +++------------------------------------------------------------+++ > Dr. G.J.W. Euverink > BioExplore, High throughput Screening Facility, > Groningen Biomolecular Sciences and Biotechnological Institute > Kerklaan 30, 9751 NN Haren, The Netherlands > phone: +31-(0)50-3632166/7633 fax: +31-(0)50-3632154 > http://www.biol.rug.nl/bioexplore/bioexplore.html (under construction) > ---++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++--- > --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: gledson@SPAMFOIL.whatman.com Subject: Re: Lab-Robotics: 384-well plates with glass bottom Date: Thu, 18 Mar 1999 09:42:52 -0500 Whatman Polyfiltronics, in collaboration with Evotec , produce a 96 well glass bottom plate. There is a growing demand for a 384 well version. We at Polyfiltronics are addressing the technical challenge of producing a 384 glass bottom plate. As always with a new product it is helpful to have an indication of the number of potential customers. If there is a genuine strong demand out there we can accelerate the R&D effort. "Dr. Martin Daffertshofer" on 03/18/99 03:26:16 AM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) cc: (bcc: Grahame Ledson/Whatman) Subject: Lab-Robotics: 384-well plates with glass bottom --------------------- Lab-Robotics --------------------- Does anyone know of a manufacturer of 384-well plates with glass bottom? Thanks Martin Daffertshofer EVOTEC BioSystems --=_X9040113073042346Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: chance@SPAMFOIL.molecumetics.com (Chance Elliott) Subject: Re:Lab-Robotics: Print and apply bar-code instrumentation Date: Thu, 18 Mar 1999 08:18:44 -0800 Hi Brendan. I have used the saigen labeler in the past (when I was at Amgen) and have since changed to the Zymark Labeler. We have been pleased with it's performance. One of the things that makes it really nice is the OCX control that is provided with the Labeler software. This allows you to develop a custom front end very easily and provides simple integration with robotic arms and stackers. Ours is on a CRS Polara system and, with the OCX control, writing the driver was a relatively simple task. We also built a custom front end to provide a little more flexibility than the standard software allows. Chance Elliott Research Automation Engineer Molecumetics LTD ____________________Reply Separator____________________ Subject: Lab-Robotics: Print and apply bar-code instrumentation Author: Dan Murphy Date: 3/18/99 8:46 AM --------------------- Lab-Robotics --------------------- Check out the Zymark Presto Labeler, it interfaces to the Twister Plate Stacker which can hold up to 80 plates. There are three options for entering the barcodes(manual, a template that is numerically incremented or a comma delimited ascii file. Once it is setup and running the Twister labeler combo has performed very well for our customers, I'm not sure of the cost but for further info check out http://www.zymark.com/MARKETIN/PRODUCTS/LABELER/Labeler.htm Thanks, Dan Murphy Zymark Corporation -----Original Message----- From: Brendan_J_Connolly@SPAMFOIL.sbphrd.com [mailto:Brendan_J_Connolly@SPAMFOIL.sbphrd.com] Sent: Wednesday, March 17, 1999 12:07 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Print and apply bar-code instrumentation --------------------- Lab-Robotics --------------------- We are looking at implementing a system to print and apply bar codes to microtiter plates and would like any information on instrumentation that can perform this task. This includes the plate capacity (Stackers) How long it takes to label 100 plates How the bar-codes are loaded into the printer (manual or file - if so what format is the file in) Cost of a system Reliability of a system Thanks Brendan --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Fred Spike, Spike International, Ltd., Wilmington, NC, USA" Subject: RE: Lab-Robotics: 384-well plates with glass bottom Date: Thu, 18 Mar 1999 11:19:26 -0500 Spike International, Ltd. carries 384-well all glass plates. Would you like information about these plates? Contact: Phone-(800)734-9408, (910)790-3380 Fax-(877)561-6015, (910)790-2023 Best regards, Scott Spike -----Original Message----- From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On Behalf Of Dr. Martin Daffertshofer Sent: Thursday, March 18, 1999 3:26 AM To: Lab-Robotics Mailing List Subject: Lab-Robotics: 384-well plates with glass bottom --------------------- Lab-Robotics --------------------- Does anyone know of a manufacturer of 384-well plates with glass bottom? Thanks Martin Daffertshofer EVOTEC BioSystems --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" From: Lenore Buehrer Subject: RE: Lab-Robotics: Flourescence Readers Date: Thu, 18 Mar 1999 11:36:49 -0500 Hello, Jeff - Bio-Tek has the FL600 Fluorescence/Absorbance reader http:\\biotek.com (capable of measuring glow luminescence reactions). The FL600 has top and bottom probes and accomodates all plates from 6 to 384 wells. Probe selection is automated through KC4 software, the operating system for the FL600. Fluorescence Sensitivity is excellent. Lenore Buehrer Bio-Tek Instruments,Inc. -----Original Message----- From: Sheldon, Adrian [mailto:ASheldon@SPAMFOIL.arqule.com] Sent: Thursday, March 18, 1999 9:39 AM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Flourescence Readers --------------------- Lab-Robotics --------------------- We have BMG fluorescence detectors. The hardware is good, and they can read 384 well plates, but our experience with the reliability and ease-of-use of the software has been disappointing. > ---------- > From: Gert-Jan Euverink[SMTP:G.J.W.Euverink@SPAMFOIL.biol.rug.nl] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Thursday, March 18, 1999 12:20 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Flourescence Readers > > --------------------- > Lab-Robotics > --------------------- > > BMG-labarotories makes a nice robot friendly fluorescense reader > (Fluorstar) http://www.bmg-labtechnologies.com/ > > However, this reader uses two filter wheels whereas the > spectramax uses two monochromaters. So fluorescense spectra > can be recorded (if support is included in the software) > > > --------------------- > > Lab-Robotics > > --------------------- > > > > > > We are trying to decide between a Tecan SpectraFlour PLUS and > > a SpectraMAX Gemini for flourescence readers. > > > > Can anyone offer some opinions/experience with these two instruments? > > > > In particular, we are concerned with bulb life, service intervals, > > validation service and expense, > > support, reliability, and performance. > > > > Our requirement is for a Q.C. instrument that can do both 96 and 384 > well > > Top read and Bottom read Flourescence. > > Are there any other players on the market in the under 30K price range? > > > > Thanks for your help! > > > > Jeff Busnach > > Automation and Multiwell Products Specialist > > Millipore Corporation > > 17 Cherry Hill Drive > > Danvers, MA > > 1-800-426-4266 x5204 > > > > > +++------------------------------------------------------------+++ > Dr. G.J.W. Euverink > BioExplore, High throughput Screening Facility, > Groningen Biomolecular Sciences and Biotechnological Institute > Kerklaan 30, 9751 NN Haren, The Netherlands > phone: +31-(0)50-3632166/7633 fax: +31-(0)50-3632154 > http://www.biol.rug.nl/bioexplore/bioexplore.html (under construction) > ---++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++--- > --=_X9040113073042346Y= Content-Type: text/plain From: "McCook, Tony" Subject: RE: Lab-Robotics: Flourescence Readers Date: Thu, 18 Mar 1999 13:07:39 -0500 Jeff, You should take a look at the VICTOR2 for EG & G Wallac. It can meet all your performance requirements. It offers the ability to measure, luminescence, absorbance and the option to measure time-resolved flourescence. The model 1420-014 is very close to your price range. There is a large installed user base and this unit has enjoys a reputation for excellent reliability and is supported by an extensive Field Service Organization. You can learn more about it at www.wallac.com or call us at 1-800-638-6692. Tony McCook Marketing Manager EG & G Wallac Inc. tmccook@SPAMFOIL.wallacus.egginc.com > -----Original Message----- > From: Gert-Jan Euverink [SMTP:G.J.W.Euverink@SPAMFOIL.biol.rug.nl] > Sent: Thursday, March 18, 1999 12:21 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Flourescence Readers > > --------------------- > Lab-Robotics > --------------------- > > BMG-labarotories makes a nice robot friendly fluorescense reader > (Fluorstar) http://www.bmg-labtechnologies.com/ > > However, this reader uses two filter wheels whereas the > spectramax uses two monochromaters. So fluorescense spectra > can be recorded (if support is included in the software) > > > --------------------- > > Lab-Robotics > > --------------------- > > > > > > We are trying to decide between a Tecan SpectraFlour PLUS and > > a SpectraMAX Gemini for flourescence readers. > > > > Can anyone offer some opinions/experience with these two instruments? > > > > In particular, we are concerned with bulb life, service intervals, > > validation service and expense, > > support, reliability, and performance. > > > > Our requirement is for a Q.C. instrument that can do both 96 and 384 > well > > Top read and Bottom read Flourescence. > > Are there any other players on the market in the under 30K price range? > > > > Thanks for your help! > > > > Jeff Busnach > > Automation and Multiwell Products Specialist > > Millipore Corporation > > 17 Cherry Hill Drive > > Danvers, MA > > 1-800-426-4266 x5204 > > > > > +++------------------------------------------------------------+++ > Dr. G.J.W. Euverink > BioExplore, High throughput Screening Facility, > Groningen Biomolecular Sciences and Biotechnological Institute > Kerklaan 30, 9751 NN Haren, The Netherlands > phone: +31-(0)50-3632166/7633 fax: +31-(0)50-3632154 > http://www.biol.rug.nl/bioexplore/bioexplore.html (under construction) > ---++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++--- --=_X9040113073042346Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: Powel Rich RJ Subject: RE: Lab-Robotics: 384-well plates with glass bottom Date: Thu, 18 Mar 1999 14:40:03 -0500 Do they have 96-well plates black wall with glass bottom? If no, can they be custom made? Rich Powel Zeneca Pharmaceuticals Wilmington, DE (302) 886-5637 > ---------- > From: Fred Spike, Spike International, Ltd., Wilmington, NC, > USA[SMTP:fredspike@SPAMFOIL.worldnet.att.net] > Sent: Thursday, March 18, 1999 11:19 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: 384-well plates with glass bottom > > --------------------- > Lab-Robotics > --------------------- > Spike International, Ltd. carries 384-well all glass plates. Would you > like > information about these plates? Contact: Phone-(800)734-9408, > (910)790-3380 Fax-(877)561-6015, (910)790-2023 > > Best regards, > > Scott Spike > > -----Original Message----- > From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On > Behalf Of Dr. Martin Daffertshofer > Sent: Thursday, March 18, 1999 3:26 AM > To: Lab-Robotics Mailing List > Subject: Lab-Robotics: 384-well plates with glass bottom > > > --------------------- > Lab-Robotics > --------------------- > Does anyone know of a manufacturer of 384-well plates with glass bottom? > > Thanks > > Martin Daffertshofer > > EVOTEC BioSystems > --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: gledson@SPAMFOIL.whatman.com Subject: Re: Lab-Robotics: 384-well plates with glass bottom Date: Thu, 18 Mar 1999 16:07:45 -0500 Yes. Whatman Polyfiltronics make white and black 96 well glass bottom plates. With and withhout a skirt. For more info contact gledson@SPAMFOIL.whatman.com Powel Rich RJ on 03/18/99 02:40:03 PM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) cc: (bcc: Grahame Ledson/Whatman) Subject: Lab-Robotics: 384-well plates with glass bottom --------------------- Lab-Robotics --------------------- Do they have 96-well plates black wall with glass bottom? If no, can they be custom made? Rich Powel Zeneca Pharmaceuticals Wilmington, DE (302) 886-5637 > ---------- > From: Fred Spike, Spike International, Ltd., Wilmington, NC, > USA[SMTP:fredspike@SPAMFOIL.worldnet.att.net] > Sent: Thursday, March 18, 1999 11:19 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: 384-well plates with glass bottom > > --------------------- > Lab-Robotics > --------------------- > Spike International, Ltd. carries 384-well all glass plates. Would you > like > information about these plates? Contact: Phone-(800)734-9408, > (910)790-3380 Fax-(877)561-6015, (910)790-2023 > > Best regards, > > Scott Spike > > -----Original Message----- > From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On > Behalf Of Dr. Martin Daffertshofer > Sent: Thursday, March 18, 1999 3:26 AM > To: Lab-Robotics Mailing List > Subject: Lab-Robotics: 384-well plates with glass bottom > > > --------------------- > Lab-Robotics > --------------------- > Does anyone know of a manufacturer of 384-well plates with glass bottom? > > Thanks > > Martin Daffertshofer > > EVOTEC BioSystems > --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "OBPW" Subject: Re: Lab-Robotics: Print and apply bar-code instrumentation Date: Thu, 18 Mar 1999 18:44:30 -0500 Dear Brendan: Oyster Bay Pump Works, Inc. offers a variety of systems to automatically apply bar codes onto plates. Available alternatives include ink jet printing directly onto the plates or print and apply using paper labels. Bar codes can be incremented automatically. Stack feeders and restackers are included as well as large accumulators. Speeds of 900 to 1000 plates per hour are typical. Other functions such as plate sealing or top printing or top labelling are also available. See web site www.obpw.com . Email: prodinfo@SPAMFOIL.obpw.com . Call: Patrick Gaillard at 516-922-3789. Fax:516-624-9253. -----Original Message----- From: Brendan_J_Connolly@SPAMFOIL.sbphrd.com To: Lab-Robotics Mailing List Date: Wednesday, March 17, 1999 9:25 PM Subject: Lab-Robotics: Print and apply bar-code instrumentation >--------------------- >Lab-Robotics >--------------------- >We are looking at implementing a system to print and apply bar codes to >microtiter plates and would like any information on instrumentation that >can perform this task. > >This includes the plate capacity (Stackers) >How long it takes to label 100 plates >How the bar-codes are loaded into the printer (manual or file - if so what >format is the file in) >Cost of a system >Reliability of a system > >Thanks > Brendan --=_X9040113073042346Y= Content-Type: text/plain;charset="Windows-1252" Content-Transfer-Encoding: 7bit From: "Dr. Martin Daffertshofer" Subject: Water HPLC tool-kit Date: Sun, 21 Mar 1999 23:33:27 +0100 Does anyone has any experiences with Waters HPLC tool-kit linked to C++ applications developed using MS Visual C++ 6.0. Thanks Martin Daffertshofer EVOTEC BioSystems --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit From: Gottfried Griesmayr Subject: RE: Lab-Robotics: 384-well plates with glass bottom Date: Tue, 23 Mar 1999 14:01:59 +0100 Hellma Optik GmbH Jena offers a 384-well microplate (quarz-glass) for DM 3.150.- HellmaOptik@SPAMFOIL.t-online.de Dr. Gottfried Griesmayr Product Manager - Rosys Anthos Phone: +43 662 857220 Mobile: +43 676 3032676 Fax: +43 662 857223 E-mail: gogr@SPAMFOIL.rosys-anthos.com Homepage: http://www.anthos-labtec.com -----Original Message----- From: Dr. Martin Daffertshofer [SMTP:daffertshofer@SPAMFOIL.evotec.de] Sent: Thursday, March 18, 1999 3:40 PM To: Lab-Robotics Mailing List Subject: Lab-Robotics: 384-well plates with glass bottom --------------------- Lab-Robotics --------------------- Does anyone know of a manufacturer of 384-well plates with glass bottom? Thanks Martin Daffertshofer EVOTEC BioSystems --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: linguagen1@SPAMFOIL.interactive.net (Richard nufkin) Subject: Re: Lab-Robotics: Flourescence Readers Date: Tue, 23 Mar 1999 10:30:51 -0500 Jeff, We have had in-house demonstrations of the PolarStar (BMG), FL600 (Bio-Tek) and CytoFluorII (PerSeptive Biosystems). Currently we use the Victor2 (EG & G Wallac) and SpectraMAX Gemini. From my experience SpectraMAX Gemini has the most user friendly software which enables one to rapidly set-up assays and analyze the data without constantly having to refer to the user manual. In addition the SpectraMAX Gemini is tunable and so does not require filters. Jay Patel, Ph.D. Senior Scientist, Linguagen Corp., 340 Kingsland Street, Nutley, NJ, Tel: (973) 662 2002 Jeff_Busnach@SPAMFOIL.Millipore.com wrote: > --------------------- > Lab-Robotics > --------------------- > > We are trying to decide between a Tecan SpectraFlour PLUS and > a SpectraMAX Gemini for flourescence readers. > > Can anyone offer some opinions/experience with these two instruments? > > In particular, we are concerned with bulb life, service intervals, > validation service and expense, > support, reliability, and performance. > > Our requirement is for a Q.C. instrument that can do both 96 and 384 well > Top read and Bottom read Flourescence. > Are there any other players on the market in the under 30K price range? > > Thanks for your help! > > Jeff Busnach > Automation and Multiwell Products Specialist > Millipore Corporation > 17 Cherry Hill Drive > Danvers, MA > 1-800-426-4266 x5204 --=_X9040113073042346Y= Content-Type: multipart/alternative;boundary="----=_NextPart_000_0001_01BE7793.965E1A80" From: "Augusto Durand" Subject: Bar-code labels Date: Fri, 26 Mar 1999 14:18:57 -0400 Hi everyone! I am looking for an automatic barcode labeling system to be used in test tubes of various sizes. Primary application will be mother-daughter replication in a pathology environment. We need three siblings from the mother tube barcoded so they can be identified back to mother. We have printing capability and will be using automated sampling to reproduce. If any of you has a reliable way of integrating a barcode system for this application, or has used (is using) something similar, I will appreciate your input. Thanks in advance. Augie Durand Director of Marketing System Design & Manufacturing Phone: 787-643-3046 Fax: 787-783-0932 Email: adurand@SPAMFOIL.caribe.net Bye for now, Augie --=_X9040113073042346Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: quoted-printable From: "John Koehler" Subject: Glass slide cleaning Date: Fri, 26 Mar 1999 11:03:59 -0800 I have a need for automatically cleaning glass slides on a continuous high = volume basis. Can anyone direct me towards appropriate hardware? Thank = you for your assistance. John Koehler DWFritz, Inc. Automation 17750 S.W. Upper Boones Ferry Road Portland, Oregon 97224-7010 ph: 503 598-9393 fax: 503 624-2799 jkoehler@SPAMFOIL.dwfritz.com www.dwfritz.com --=_X9040113073042346Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: "John Petracca" Subject: Re: Lab-Robotics: Glass slide cleaning Date: Sun, 28 Mar 1999 03:44:05 -0500 John, If you cannot find an off the shelf solution, please contact me. I am the President of jPi, Inc. We specialize in custom engineered solutions for laboratory automation and would be happy to discuss and/or look closer at your application. Sincerely, John Petracca jPi, Inc. 67 Woodland Meadow Drive Lancaster, MA 01523 (978) 365-7487 email here or to: jpi_inc@SPAMFOIL.yahoo.com ----- Original Message ----- From: John Koehler To: Lab-Robotics Mailing List Sent: Friday, March 26, 1999 2:03 PM Subject: Lab-Robotics: Glass slide cleaning --------------------- Lab-Robotics --------------------- I have a need for automatically cleaning glass slides on a continuous high volume basis. Can anyone direct me towards appropriate hardware? Thank you for your assistance. John Koehler DWFritz, Inc. Automation 17750 S.W. Upper Boones Ferry Road Portland, Oregon 97224-7010 ph: 503 598-9393 fax: 503 624-2799 jkoehler@SPAMFOIL.dwfritz.com www.dwfritz.com --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Fred Spike, Spike International, Ltd., Wilmington, NC, USA" Subject: RE: Lab-Robotics: 384-well plates with glass bottom Date: Sun, 28 Mar 1999 06:31:36 -0500 We stock these plates in the US. Fred Spike, President Spike International, Ltd PO Box 15410 Wilmington, NC 28408 USA fred@SPAMFOIL.spike.cc www.spike.cc Phone: (800) 734-9408 Toll-free Fax: (877) 561-6015 >From outside the US: Phone: (910) 790-3380 Fax: (910) 790-2023 -----Original Message----- From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On Behalf Of Gottfried Griesmayr Sent: Tuesday, March 23, 1999 8:02 AM To: Lab-Robotics Mailing List Subject: Lab-Robotics: 384-well plates with glass bottom --------------------- Lab-Robotics --------------------- Hellma Optik GmbH Jena offers a 384-well microplate (quarz-glass) for DM 3.150.- HellmaOptik@SPAMFOIL.t-online.de Dr. Gottfried Griesmayr Product Manager - Rosys Anthos Phone: +43 662 857220 Mobile: +43 676 3032676 Fax: +43 662 857223 E-mail: gogr@SPAMFOIL.rosys-anthos.com Homepage: http://www.anthos-labtec.com -----Original Message----- From: Dr. Martin Daffertshofer [SMTP:daffertshofer@SPAMFOIL.evotec.de] Sent: Thursday, March 18, 1999 3:40 PM To: Lab-Robotics Mailing List Subject: Lab-Robotics: 384-well plates with glass bottom --------------------- Lab-Robotics --------------------- Does anyone know of a manufacturer of 384-well plates with glass bottom? Thanks Martin Daffertshofer EVOTEC BioSystems --=_X9040113073042346Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics) Subject: Thank you for subscribing to Lab-Robotics Date: Mon, 29 Mar 1999 09:31:44 -0500 Thank you for subscribing to the Lab-Robotics mailing list. This message is sent once every 30 days to remind you of the commands which Arrow (our mailing list software) understands. To unsubscribe from the list, send a message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. To change to digest mode and receive messages once a day send a message with DIGEST-1 as the subject. To receive a digest once a week, send a message with a subject of DIGEST-7. To change from digest mode back to individual messages, send a message with DIGEST-OFF as the subject. For help with these and other Arrow commands, send a message to discussion@SPAMFOIL.lab-robotics.org with just HELP as the subject. Note: All Arrow commands are a single word (with no spaces) and are sent in the subject of the message to the list. Andy Zaayenga Moderator, The Lab-Robotics Discussion Mailing List mailto:andy.zaayenga@SPAMFOIL.lab-robotics.org web site: https://www.lab-robotics.org --- Age of List in Days 183 Number of Subscribers 582 Total Number of Messages Posted to List 411 Average Number of Messages Posted Per Day 2 --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: Ed Kuzniar Subject: Centrifuges and vortexers Date: Mon, 29 Mar 1999 15:41:52 +0000 I would be interested to hear about the following: 1. Small centrifuges for deep / shallow well, foil sealed plates. The application is to ensure that solutions are sitting on the bottom of the wells prior to piercing the plate seals and subsequent replication. Any ideas on the spec needed, including centrifuge speed and time? 2. Small vortexers for dissolving evaporated samples in deep well plates. Can you recommend any equipment for the above? Ed Kuzniar Phone: +44 1763 262026 Ext: 215 Fax: +44 1763 262613 MailTo:ejak@SPAMFOIL.autoprt.co.uk http://www.autoprt.co.uk ================================ The Automation Partnership (Cambridge) Limited Registered Office: Melbourn Science Park Melbourn, Royston Hertfordshire SG8 6HB UK Registered in England, Company No: 2823205 --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "dxs354@SPAMFOIL.psu.edu" Subject: Yttrium Silicate SPA Beads Date: Mon, 29 Mar 1999 22:14:06 -0500 I was curious if anyone has had trouble dispensing a suspension of yttrium silicate SPA beads? They seem to settle very quickly and stick to tubing. Are there any satisfactory solutions to this problem? Thanks, Matt --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: "Susan T Ballard" Subject: 96 to 384 technical issues Date: Tue, 30 Mar 1999 10:26:50 -0800 Could anyone provide a reference(s) for an anticle(s) relating to the technical issues of moving ELISA assays from 96 to 384 well format? Any help would be appreciated! This information is per request of one of my customers. Thanks, Susan Ballard Automated Solutions Consultant Drug Discovery, SouthEast Beckman Coulter stballard@SPAMFOIL.beckman.com 800-392-2164, ext 9893 --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii From: Jeff_Busnach@SPAMFOIL.Millipore.com Subject: Re: Lab-Robotics: Yttrium Silicate SPA Beads Date: Tue, 30 Mar 1999 10:22:57 -0500 Matt, You can try changing the Specific Gravity of you suspension solution. This can help keep a suspended particle from settling. I'm not sure how effective it is with Silicates, or how sensitive SPA beads would be to a solvent. --=_X9040113073042346Y= Content-Type: text/plain; charset="us-ascii" From: "Thomas J. Baiga" Subject: Re: Lab-Robotics: Yttrium Silicate SPA Beads Date: Tue, 30 Mar 1999 22:39:09 -0800 At 10:14 PM 3/29/99 -0500, you wrote: >--------------------- >Lab-Robotics >--------------------- >I was curious if anyone has had trouble dispensing a suspension of yttrium >silicate SPA beads? They seem to settle very quickly and stick to tubing. >Are there any satisfactory solutions to this problem? > >Thanks, >Matt > > Dear Matt, Could you please be a little more specific. Information such as the method of dispensing (what instrumentation, etc.), type and composition of tubing, materials of contact for entire liquid handling procedure, solvent(s), and additional specs on the SPA beads. The more info you share, the more helpful replies the various liquid handling guru's around here can offer. Tom ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ --=_X9040113073042346Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: Ken Bunn Subject: RE: Lab-Robotics: 96 to 384 technical issues Date: Wed, 31 Mar 1999 04:43:02 -0500 HI Susan, when we developed the 384 well plate and Nalge Nunc we did extensive work in Micro ELISA from 96 to 384 well. The advantages are significant and a paper has been written on the attributes of the 384 well geometry. I will call you today to follow up. -----Original Message----- From: Susan T Ballard [SMTP:stballard@SPAMFOIL.beckman.com] Sent: Tuesday, March 30, 1999 1:27 PM To: Lab-Robotics Mailing List Subject: Lab-Robotics: 96 to 384 technical issues --------------------- Lab-Robotics --------------------- Could anyone provide a reference(s) for an anticle(s) relating to the technical issues of moving ELISA assays from 96 to 384 well format? Any help would be appreciated! This information is per request of one of my customers. Thanks, Susan Ballard Automated Solutions Consultant Drug Discovery, SouthEast Beckman Coulter stballard@SPAMFOIL.beckman.com 800-392-2164, ext 9893 --=_X9040113073042346Y= Content-Type: text/plain From: Kevin Olsen Subject: A 384 Well Mathematical Puzzle Date: Wed, 31 Mar 1999 08:44:16 -0500 Dear Fellow LRIG Members, Like many of you we are making 384 well microplates on a routine basis. Most of the 384 well plates have been made by combining aliquots from four 96 well plates. Each 96 well plate has a five digit number (if made internally) or a six digit number (if made by an outside collaborator.) For example, suppose we combine aliquots from the following 96 well plates: 12345 12346 12347 12348 The problem is, what do we call the new plate? If we call it 12345-12346-12347-12348, the label will be longer than the plate itself. If we give it a new number entirely, for example 54321, then there is no easy way to reference the original plates. So here is the puzzle. Is there a quick algorithm whereby the original four numbers can be combined into one new number? And once this is done, can this new number be readily converted back to the original four numbers? (Using two-dimensional bar code labels is not allowed.) The same problem occurs when we make 1536 well plates by combining aliquots from 16, 96 well plates. One possible solution was proposed by Bart Zoltan of our Biomedical Engineering department. The new number's first five digits are the ID of the plate with the lowest ID number. The next three digits are the increment from the last plate. Thus in the example cited above, the number of the 384 well plate becomes: 12345-111 It's a really elegant solution because the operator can mentally convert back into the original plate numbers very easily. But the problem arises with a set of source plates with more than 9 numbers between any two of them. What happens if a screening laboratory decides that they want to pick any four numbers out of several hundred? If the subscribers on this list are unaware of a suitable algorithm, does anyone know of any cryptographers with some free time? Thank you, Kevin Olsen Wyeth Ayerst Research Compound Bank Pearl River, NY, 10965 914-732-3392 (This problem is also posted on the LRIG Discussion Web.) --=_X9040113073042346Y= Content-Type: text/plain From: Xu Min Subject: RE: Lab-Robotics: 96 to 384 technical issues Date: Wed, 31 Mar 1999 08:47:19 -0500 Hi Ken: Can you forward me a copy of the paper? I am interested in 96 to 384 ELISA too. Min > -----Original Message----- > From: Ken Bunn [SMTP:kbunn@SPAMFOIL.ix.netcom.com] > Sent: Wednesday, March 31, 1999 4:43 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: 96 to 384 technical issues > > --------------------- > Lab-Robotics > --------------------- > HI Susan, when we developed the 384 well plate and Nalge Nunc we did > extensive work in Micro ELISA from 96 to 384 well. The advantages are > significant and a paper has been written on the attributes of the 384 well > > geometry. I will call you today to follow up. > > -----Original Message----- > From: Susan T Ballard [SMTP:stballard@SPAMFOIL.beckman.com] > Sent: Tuesday, March 30, 1999 1:27 PM > To: Lab-Robotics Mailing List > Subject: Lab-Robotics: 96 to 384 technical issues > > --------------------- > Lab-Robotics > --------------------- > Could anyone provide a reference(s) for an anticle(s) relating to the > technical issues of moving ELISA assays from 96 to 384 well format? Any > help would be appreciated! This information is per request of one of my > customers. > > Thanks, > > Susan Ballard > Automated Solutions Consultant > Drug Discovery, SouthEast > Beckman Coulter > stballard@SPAMFOIL.beckman.com > 800-392-2164, ext 9893 --=_X9040113073042346Y= Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: quoted-printable From: Mark F Russo Subject: Re: Lab-Robotics: A 384 Well Mathematical Puzzle Date: Wed, 31 Mar 1999 10:25:35 -0500 Kevin: If your barcodes are all numeric, you might consider the following. - Convert the barcode string into an equivalent binary number. 3-bytes w= ill store any 7-digit barcode from 0000000 to 9999999. - Concatenate the bytes representing all barcodes. Five 7-digit barcodes= times 3 bytes each equals 15 bytes of data. - Then use a standard algorithm to convert the concatenated bytes into pr= intable characters. For example, consider the uuencode algorithm or base= -64 encoding. If I remember correctly, uuencode expands the size of enco= ded data to about 8/7 the original size. Base-64 expands to 4/3 the orig= inal size. Using this approach, five 7-digit barcodes (35 digits) could be encoded i= n 18 printable characters using uuencode, and 20 printable charactes usin= g base-64. Of course, the downside is that the barcode would not be easily readable = by a human. Mark Russo Kevin Olsen wrote: > --------------------- > Lab-Robotics > --------------------- > Dear Fellow LRIG Members, > > Like many of you we are making 384 well microplates on a routine basis.= Most of the 384 well plates have been made by combining aliquots from f= our 96 well plates. Each 96 well plate has a five digit number (if made = internally) or a six digit number (if made by an outside collaborator.) > > For example, suppose we combine aliquots from the following 96 well pla= tes: > > 12345 > 12346 > 12347 > 12348 > > The problem is, what do we call the new plate? If we call it 12345-123= 46-12347-12348, the label will be longer than the plate itself. If we gi= ve it a new number entirely, for example 54321, then there is no easy way= to reference the original plates. > > So here is the puzzle. Is there a quick algorithm whereby the original= four numbers can be combined into one new number? And once this is done= , can this new number be readily converted back to the original four numb= ers? (Using two-dimensional bar code labels is not allowed.) > > The same problem occurs when we make 1536 well plates by combining aliq= uots from 16, 96 well plates. > > One possible solution was proposed by Bart Zoltan of our Biomedical Eng= ineering department. The new number's first five digits are the ID of th= e plate with the lowest ID number. The next three digits are the increme= nt from the last plate. Thus in the example cited above, the number of t= he 384 well plate becomes: > > 12345-111 > > It's a really elegant solution because the operator can mentally conver= t back into the original plate numbers very easily. > > But the problem arises with a set of source plates with more than 9 num= bers between any two of them. What happens if a screening laboratory dec= ides that they want to pick any four numbers out of several hundred? > > If the subscribers on this list are unaware of a suitable algorithm, do= es anyone know of any cryptographers with some free time? > > Thank you, > > Kevin Olsen > Wyeth Ayerst Research > Compound Bank > Pearl River, NY, 10965 > > 914-732-3392 > > (This problem is also posted on the LRIG Discussion Web.) > = = = = = = = = = = = = = ! > --=_X9040113073042346Y= Content-Type: text/plain; charset="iso-8859-1" From: Patrick Cleveland Subject: re: Lab-Robotics: Yttrium Silicate SPA Beads Date: Wed, 31 Mar 1999 12:55:50 -0800 Matt, You can keep Yttrium silicate SPA beads in suspension using the V&P Scientific Tumble Stir Reservoirs with a Magnetic Rotary Tumble Stirrer. These beads are very dense and settle quickly so they require a very vigorous stirring. This system does it. We have a web page with photos and a description of the system see http://www.vp-scientific.com/stir%20disc%20and%20paddle%20troughs.htm Call me if you have any questions. Patrick Cleveland, Ph.D. President V&P Scientific, Inc. 9853 Pacific Heights Blvd., Suite N San Diego, CA 92121 Phone (619) 455-0643, toll free (800) 455-0644 FAX (619) 455-0703 e-mail Check out our catalog and web site at http://www.vp-scientific.com/ --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "dxs354@SPAMFOIL.psu.edu" Subject: Re: Lab-Robotics: A 384 Well Mathematical Puzzle Date: Wed, 31 Mar 1999 17:45:39 -0500 Why wouldn't there be an easy way to reference the original plates. We have a separate number system (barcode) for mother/daughter plates. This way when a daughter plate is created it is linked to the mother plate in a database (or 4 mother plates for a 384 daughter plate). Matt Smicker HTS Scientist Rhone-Poulenc Rorer ----- Original Message ----- From: Kevin Olsen To: Lab-Robotics Mailing List Sent: Wednesday, March 31, 1999 8:44 AM Subject: Lab-Robotics: A 384 Well Mathematical Puzzle > --------------------- > Lab-Robotics > --------------------- > Dear Fellow LRIG Members, > > Like many of you we are making 384 well microplates on a routine basis. Most of the 384 well plates have been made by combining aliquots from four 96 well plates. Each 96 well plate has a five digit number (if made internally) or a six digit number (if made by an outside collaborator.) > > For example, suppose we combine aliquots from the following 96 well plates: > > 12345 > 12346 > 12347 > 12348 > > The problem is, what do we call the new plate? If we call it 12345-12346-12347-12348, the label will be longer than the plate itself. If we give it a new number entirely, for example 54321, then there is no easy way to reference the original plates. > > So here is the puzzle. Is there a quick algorithm whereby the original four numbers can be combined into one new number? And once this is done, can this new number be readily converted back to the original four numbers? (Using two-dimensional bar code labels is not allowed.) > > The same problem occurs when we make 1536 well plates by combining aliquots from 16, 96 well plates. > > One possible solution was proposed by Bart Zoltan of our Biomedical Engineering department. The new number's first five digits are the ID of the plate with the lowest ID number. The next three digits are the increment from the last plate. Thus in the example cited above, the number of the 384 well plate becomes: > > 12345-111 > > It's a really elegant solution because the operator can mentally convert back into the original plate numbers very easily. > > But the problem arises with a set of source plates with more than 9 numbers between any two of them. What happens if a screening laboratory decides that they want to pick any four numbers out of several hundred? > > If the subscribers on this list are unaware of a suitable algorithm, does anyone know of any cryptographers with some free time? > > Thank you, > > Kevin Olsen > Wyeth Ayerst Research > Compound Bank > Pearl River, NY, 10965 > > 914-732-3392 > > (This problem is also posted on the LRIG Discussion Web.) > ! > ! > > > > --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "dxs354@SPAMFOIL.psu.edu" Subject: Re: Lab-Robotics: Yttrium Silicate SPA Beads Date: Wed, 31 Mar 1999 17:53:35 -0500 We were using a Labsystems Multidrop. The "solvent" is water, and I believe the tubing is coated or made of some type of silicon. We had two major problems using this instrument. 1) The beads settled somewhat in between dispensing, causing some columns to get less beads. 2) The tubing clogged fairly often. I would really like to use a Zymark Rapidplate to dispense, but keeping the beads suspended seems to be the problem there. Matt Smicker HTS Scientist Rhone-Poulenc Rorer ----- Original Message ----- From: Thomas J. Baiga To: Lab-Robotics Mailing List Sent: Wednesday, March 31, 1999 1:39 AM Subject: Lab-Robotics: Yttrium Silicate SPA Beads > --------------------- > Lab-Robotics > --------------------- > At 10:14 PM 3/29/99 -0500, you wrote: > >--------------------- > >Lab-Robotics > >--------------------- > >I was curious if anyone has had trouble dispensing a suspension of yttrium > >silicate SPA beads? They seem to settle very quickly and stick to tubing. > >Are there any satisfactory solutions to this problem? > > > >Thanks, > >Matt > > > > > Dear Matt, > > Could you please be a little more specific. Information such as the method > of dispensing (what instrumentation, etc.), type and composition of tubing, > materials of contact for entire liquid handling procedure, solvent(s), and > additional specs on the SPA beads. The more info you share, the more helpful > replies the various liquid handling guru's around here can offer. > > Tom > ----------------------------------------------------------------------- > Thomas J. Baiga > President and Chief Executive Officer > > Charybdis Technologies, Inc. > 5925 Priestly Drive > Suite 101 > Carlsbad, California 92008 > > Phone: 760.930.6100 > Fax: 760.930.6099 > > tjbaiga@SPAMFOIL.charybtech.com > http://www.charybtech.com/ > > --=_X9040113073042346Y= Content-Type: text/plain;charset=iso-8859-1 Content-Transfer-Encoding: 7bit From: PETER.NIGGEMANN@SPAMFOIL.monsanto.com Subject: RE: Lab-Robotics: A 384 Well Mathematical Puzzle Date: 31 Mar 1999 17:00:00 -0600 Kevin, It is best if you use a new plate number and add a field to your database that references your 4 or 16, 96 well plates. You get into to much trouble when you try to store data in a plate identifier. After all what happens when you discover that plate a+b+c+d was really plate a+b+e+d? If it is coded only in the identifier then it will be wrong forever or you will have to remove it from all assays and hope there is no more data floating around associated to the identifier. If it is coded in a separate field then all you need to do is update the one field and now it is correct for all assays. If you want the user to be able to see the other identifiers on the label then make those part of the human readable portion of the barcode only. Take Care > -----Original Message----- > From: discussion@SPAMFOIL.lab-robotics.org at INTERNET > Sent: Wednesday, March 31, 1999 9:23 AM > To: discussion@SPAMFOIL.lab-robotics.org at INTERNET > Subject: Lab-Robotics: A 384 Well Mathematical Puzzle > > > --------------------- > Lab-Robotics > --------------------- > Dear Fellow LRIG Members, > > Like many of you we are making 384 well microplates on a > routine basis. Most of > the 384 well plates have been made by combining aliquots from > four 96 well > plates. Each 96 well plate has a five digit number (if made > internally) or a > six digit number (if made by an outside collaborator.) > > For example, suppose we combine aliquots from the following > 96 well plates: > > 12345 > 12346 > 12347 > 12348 > > The problem is, what do we call the new plate? If we call it > 12345-12346-12347-12348, the label will be longer than the > plate itself. If we > give it a new number entirely, for example 54321, then there > is no easy way to > reference the original plates. > > So here is the puzzle. Is there a quick algorithm whereby > the original four > numbers can be combined into one new number? And once this > is done, can this > new number be readily converted back to the original four > numbers? (Using > two-dimensional bar code labels is not allowed.) > > The same problem occurs when we make 1536 well plates by > combining aliquots from > 16, 96 well plates. > > One possible solution was proposed by Bart Zoltan of our > Biomedical Engineering > department. The new number's first five digits are the ID of > the plate with the > lowest ID number. The next three digits are the increment > from the last plate. > Thus in the example cited above, the number of the 384 well > plate becomes: > > 12345-111 > > It's a really elegant solution because the operator can > mentally convert back > into the original plate numbers very easily. > > But the problem arises with a set of source plates with more > than 9 numbers > between any two of them. What happens if a screening > laboratory decides that > they want to pick any four numbers out of several hundred? > > If the subscribers on this list are unaware of a suitable > algorithm, does anyone > know of any cryptographers with some free time? > > Thank you, > > Kevin Olsen > Wyeth Ayerst Research > Compound Bank > Pearl River, NY, 10965 > > 914-732-3392 > > (This problem is also posted on the LRIG Discussion Web.) > > > > > > > > > > > > > > > > > > > > > > > > > ! > > > > > > > > > > > > > > > > > > > > > > > --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "OBPW" Subject: Re: Lab-Robotics: A 384 Well Mathematical Puzzle Date: Thu, 1 Apr 1999 08:49:33 -0500 If one could rely on a continuous series of numbers, the solution would be simple. It is the requirement to allow for practically random groups of numbers which requires calling in the cryptographers. In reality, the original premise that a label with all these numbers on it would be longer than the plate is only true if the font size is too large. There is actually room on the short side of a plate to print 4 readable lines of text with 4 numbers (of 5 or even more digits each) for a total of 16 numbers. We design and build custom microplate processing automation systems and use ink jet printers to print directly onto plates without labels as well as applying printed labels. Even with ink jet printers which offer less resolution than printed labels, we can print up to three rows of 6 characters each onto the tabs at the ends of removable 8 way strips for 96 well plates. These tabs only offer about 1/4 square inch of space to print on. Don't wish to spoil the party for Mathematicians but, smaller print also eliminates the need for applying reverse algorithm to decipher numbers. Patrick Gaillard Oyster Bay Pump Works, Inc. prodinfo@SPAMFOIL.obpw.com -----Original Message----- From: Kevin Olsen To: Lab-Robotics Mailing List Date: Wednesday, March 31, 1999 9:53 AM Subject: Lab-Robotics: A 384 Well Mathematical Puzzle >--------------------- >Lab-Robotics >--------------------- >Dear Fellow LRIG Members, > >Like many of you we are making 384 well microplates on a routine basis. Most of the 384 well plates have been made by combining aliquots from four 96 well plates. Each 96 well plate has a five digit number (if made internally) or a six digit number (if made by an outside collaborator.) > >For example, suppose we combine aliquots from the following 96 well plates: > >12345 >12346 >12347 >12348 > >The problem is, what do we call the new plate? If we call it 12345-12346-12347-12348, the label will be longer than the plate itself. If we give it a new number entirely, for example 54321, then there is no easy way to reference the original plates. > >So here is the puzzle. Is there a quick algorithm whereby the original four numbers can be combined into one new number? And once this is done, can this new number be readily converted back to the original four numbers? (Using two-dimensional bar code labels is not allowed.) > >The same problem occurs when we make 1536 well plates by combining aliquots from 16, 96 well plates. > >One possible solution was proposed by Bart Zoltan of our Biomedical Engineering department. The new number's first five digits are the ID of the plate with the lowest ID number. The next three digits are the increment from the last plate. Thus in the example cited above, the number of the 384 well plate becomes: > >12345-111 > >It's a really elegant solution because the operator can mentally convert back into the original plate numbers very easily. > >But the problem arises with a set of source plates with more than 9 numbers between any two of them. What happens if a screening laboratory decides that they want to pick any four numbers out of several hundred? > >If the subscribers on this list are unaware of a suitable algorithm, does anyone know of any cryptographers with some free time? > >Thank you, > >Kevin Olsen >Wyeth Ayerst Research >Compound Bank >Pearl River, NY, 10965 > >914-732-3392 > >(This problem is also posted on the LRIG Discussion Web.) > ! >! > > --=_X9040113073042346Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Ernie Woods" Subject: Re: Lab-Robotics: Yttrium Silicate SPA Beads Date: Thu, 1 Apr 1999 09:56:35 -0500 Matt, We also used the Multidrop to transport beads from beaker to multiwell plate, and with great success.This was for a pilot manufacturing process. The beads were ~20um silica and (agarose) gel, transported during separate runs. The suspension buffer was water. We played with the specific gravity by adding salt, but results were not worth the effort. We used a liquid to solids ratio of ~75/25 for silica and 50/50 for gel, determined by suspension characteristics. We loaded into single prep columns and 96 and 384 well filter plates. With the 384 plates, we found that a dispense pattern available through PC control yielded better alignment (plate wells to dispense tip) as the weight of the 384 plate with liquid is close to the limit of the conveyor system. We used an impeller to stir the mixture in the beaker (~200-500ml) to keep the solids in suspension, but not spinning so fast as to cause cavitation (aeration) and used a rigid support to hold the inlet tubing assembly along the edge so it wouldn't get mixed up in the blades of the impeller. If the material sat for more than 10-15 seconds in the tubing between plates, we emptied the lines then re-primed and started again. And yes, some material stuck to the walls of the (silicone) tubing, in the beginning, then all 'sites' were taken up and delivery volumes were (very) precise and reproducible. We had a tubing set for each bead type. I feel the rapid plate with 96 channel tips will clog far more frequently than the Multidrop, as we tried similar products during our research and you will need to address the issue of bead suspension in the trough. Good luck. Ernie Woods Hudson Control Group www.hudsoncontrol.com ----- Original Message ----- From: dxs354@SPAMFOIL.psu.edu To: Lab-Robotics Mailing List Sent: Wednesday, March 31, 1999 17:53 Subject: Lab-Robotics: Yttrium Silicate SPA Beads > --------------------- > Lab-Robotics > --------------------- > We were using a Labsystems Multidrop. The "solvent" is water, and I believe > the tubing is coated or made of some type of silicon. > > We had two major problems using this instrument. 1) The beads settled > somewhat in between dispensing, causing some columns to get less beads. 2) > The tubing clogged fairly often. > > I would really like to use a Zymark Rapidplate to dispense, but keeping the > beads suspended seems to be the problem there. > > Matt Smicker > HTS Scientist > Rhone-Poulenc Rorer > > ----- Original Message ----- > From: Thomas J. Baiga > To: Lab-Robotics Mailing List > Sent: Wednesday, March 31, 1999 1:39 AM > Subject: Lab-Robotics: Yttrium Silicate SPA Beads > > > > --------------------- > > Lab-Robotics > > --------------------- > > At 10:14 PM 3/29/99 -0500, you wrote: > > >--------------------- > > >Lab-Robotics > > >--------------------- > > >I was curious if anyone has had trouble dispensing a suspension of > yttrium > > >silicate SPA beads? They seem to settle very quickly and stick to > tubing. > > >Are there any satisfactory solutions to this problem? > > > > > >Thanks, > > >Matt > > > > > > > > Dear Matt, > > > > Could you please be a little more specific. Information such as the > method > > of dispensing (what instrumentation, etc.), type and composition of > tubing, > > materials of contact for entire liquid handling procedure, solvent(s), and > > additional specs on the SPA beads. The more info you share, the more > helpful > > replies the various liquid handling guru's around here can offer. > > > > Tom > > ----------------------------------------------------------------------- > > Thomas J. Baiga > > President and Chief Executive Officer > > > > Charybdis Technologies, Inc. > > 5925 Priestly Drive > > Suite 101 > > Carlsbad, California 92008 > > > > Phone: 760.930.6100 > > Fax: 760.930.6099 > > > > tjbaiga@SPAMFOIL.charybtech.com > > http://www.charybtech.com/ > > > > > > --=_X9040113073042346Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit --=_X9040113073042346Y=--