This is a multi-part MIME document. --=_X9022820412355328Y= Content-Type: text/plain From: "BODE, Donald C." Subject: Alternate sources of Beckman Coulter pipet tips? Date: Mon, 1 Feb 1999 15:21:44 -0500 I would like to find an alternate source of pipet tips comparable to the Beckman Coulter P200 tips ("Sagian Type 2"), Catalog No. 148005, more for reasons of supply than cost. Thanks, Chris Bode Rhone-Poulenc Rorer Collegeville, PA --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Carlo J van Staden Subject: Re: Lab-Robotics: Alternate sources of Beckman Coulter pipet tips? Date: Mon, 1 Feb 1999 15:09:54 -0500 You can call Robbins Scientific (Cat#1043-04-5) and also E&K Scientific (part #?). However, if you want to use the Robbins tips on the Multimek, you will have to change the tip loading guides which are on the P200 head. Carlo van Staden >--------------------- >Lab-Robotics >--------------------- >I would like to find an alternate source of pipet tips comparable to the >Beckman Coulter P200 tips ("Sagian Type 2"), Catalog No. 148005, more for >reasons of supply than cost. > >Thanks, > >Chris Bode >Rhone-Poulenc Rorer >Collegeville, PA ____________________________________ Carlo J van Staden Manager of Antimicrobial Screening SCRIPTGEN Pharmaceuticals Inc., 610 Lincoln Street, Waltham, Massachusetts USA 02451 PHONE: (781)768-3448 FAX: (781)768-0040 mailto:cvanstaden@SPAMFOIL.scriptgen.com ____________________________________ --=_X9022820412355328Y= Content-Type: text/plain From: "Jabado, Jennifer [PRI]" Subject: correct tare weights of vials Date: Mon, 1 Feb 1999 17:29:27 -0500 A co-worker of mine is investigating the reproducibility of vial tare weights. In her own experiments , she has found a significant (compared to our sample sizes) difference in tare weights of empty, labeled and capped vials. By adjusting the ambient humidity, she has seen reproducible weight variations as great as 15 miliigrams. Since our samples are often 15 milligrams or less, you can see that this is a significant problem for us. She has been able to determine that the problem is either the label or the cap. She will do further experiments some time soon to determine if one or both of them are the culprit. > Has anyone addressed this problem and if so, how have you been able to > solve it? Do you dry the vials before taring and reweighing? Do you use > materials that don't absorb water? Would you be willing to share that > information with us? Do you know if other people have addressed this > question? > Thanks, Jennifer Jabado Associate Scientist R.W.Johnson Pharmaceutical Research Institute 1000 Rt 202, PO Box 300, Raritan, NJ 08869 phone 908-704-4954, fax 908-526-6469 email jjabado@SPAMFOIL.prius.jnj.com & Susan Hadden Senior Associate Scientist R. W. Johnson Pharmaceutical Research Institute 1000 Rt 202, PO Box 300, Raritan, NJ 08869 phone 908-704-4295, fax 908-526-6469 email shadden@SPAMFOIL.prius.jnj.com --=_X9022820412355328Y= Content-Type: text/plain; CHARSET=US-ASCII From: "Ramesha, Chakk {RES~Palo Alto}" Subject: RE: Lab-Robotics: Alternate sources of Beckman Coulter pipet tips ? Date: Mon, 01 Feb 1999 16:41:02 -0800 Check with E&K. We get tips from them. Works well. C. Ramesha Roche > -----Original Message----- > From: BODE, Donald C. [SMTP:Donald.Bode@SPAMFOIL.rp-rorer.com] > Sent: Monday, February 01, 1999 12:22 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Alternate sources of Beckman Coulter pipet > tips? > > --------------------- > Lab-Robotics > --------------------- > I would like to find an alternate source of pipet tips comparable to the > Beckman Coulter P200 tips ("Sagian Type 2"), Catalog No. 148005, more for > reasons of supply than cost. > > Thanks, > > Chris Bode > Rhone-Poulenc Rorer > Collegeville, PA --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: md@SPAMFOIL.molecumetics.com (Mark Deiparine) Subject: Re:Lab-Robotics: Alternate sources of Beckman Coulter pipet Date: Mon, 1 Feb 1999 17:45:42 -0800 Also check into Molecular BioProducts, if these tips are for a MultiMek or BioMek. Molecular BioProducts 888.908.5325 htp://www.mbpinc.com --=_X9022820412355328Y= Content-Type: text/plain From: "Duncan, Mike E" Subject: RE: Lab-Robotics: correct tare weights of vials Date: Tue, 2 Feb 1999 09:24:24 -0000 You could talk to Andrew Whitwell at Labman; his company have some experience in this area. You can get him at: andrew@SPAMFOIL.labman.co.uk Regards, Mike Duncan > -----Original Message----- > From: Jabado, Jennifer [PRI] [SMTP:JJabado@SPAMFOIL.prius.jnj.com] > Sent: 01 February 1999 22:29 > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: correct tare weights of vials > > --------------------- > Lab-Robotics > --------------------- > > A co-worker of mine is investigating the reproducibility of vial > tare weights. In her own experiments , she has found a significant > (compared > to our sample sizes) difference in tare weights of empty, labeled and > capped > vials. By adjusting the ambient humidity, she has seen reproducible weight > variations as great as 15 miliigrams. Since our samples are often 15 > milligrams or less, you can see that this is a significant problem for us. > She has been able to determine that the problem is either the label or the > cap. She will do further experiments some time soon to determine if one or > both of them are the culprit. > > > Has anyone addressed this problem and if so, how have you been able to > > solve it? Do you dry the vials before taring and reweighing? Do you use > > materials that don't absorb water? Would you be willing to share that > > information with us? Do you know if other people have addressed this > > question? > > Thanks, > > Jennifer Jabado > Associate Scientist > R.W.Johnson Pharmaceutical Research Institute > 1000 Rt 202, PO Box 300, Raritan, NJ 08869 > phone 908-704-4954, fax 908-526-6469 > email jjabado@SPAMFOIL.prius.jnj.com > & > Susan Hadden > Senior Associate Scientist > R. W. Johnson Pharmaceutical Research Institute > 1000 Rt 202, PO Box 300, Raritan, NJ 08869 > phone 908-704-4295, fax 908-526-6469 > email shadden@SPAMFOIL.prius.jnj.com --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: petek@SPAMFOIL.pharmacop.com (Kieselbach, Pete) Subject: Re: Lab-Robotics: correct tare weights of vials Date: Tue, 2 Feb 1999 08:16:32 -0500 Jennifer: John Wetzel started a thread on this subject back in October (see below). You might check with him to see if he has had any success. Pete Comments: Authenticated sender is From: "Wetzel" To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) Date: Fri, 23 Oct 1998 15:30:30 +0000 MIME-Version: 1.0 Subject: Lab-Robotics: weight loss of polypropylene tubes Priority: normal Reply-To: discussion@SPAMFOIL.lab-robotics.org Sender: discussion@SPAMFOIL.lab-robotics.org --------------------- Lab-Robotics --------------------- Thanks to Peter and David for your replies. We have tried preconditioning, and found that weight loss continued after 9 hours under vacuum at 65 C. After 18 hours, weight loss averaged 0.7 mg/tube. Our studies on whether the lost weight returned over time have so far been inconclusive, so we have to do more experiments with tighter control of humidity and vacuum pressure. John Wetzel >--------------------- >Lab-Robotics >--------------------- > > A co-worker of mine is investigating the reproducibility of vial >tare weights. In her own experiments , she has found a significant (compared >to our sample sizes) difference in tare weights of empty, labeled and capped >vials. By adjusting the ambient humidity, she has seen reproducible weight >variations as great as 15 miliigrams. Since our samples are often 15 >milligrams or less, you can see that this is a significant problem for us. >She has been able to determine that the problem is either the label or the >cap. She will do further experiments some time soon to determine if one or >both of them are the culprit. > >> Has anyone addressed this problem and if so, how have you been able to >> solve it? Do you dry the vials before taring and reweighing? Do you use >> materials that don't absorb water? Would you be willing to share that >> information with us? Do you know if other people have addressed this >> question? >> Thanks, > > Jennifer Jabado > Associate Scientist > R.W.Johnson Pharmaceutical Research Institute > 1000 Rt 202, PO Box 300, Raritan, NJ 08869 > phone 908-704-4954, fax 908-526-6469 > email jjabado@SPAMFOIL.prius.jnj.com >& >Susan Hadden >Senior Associate Scientist >R. W. Johnson Pharmaceutical Research Institute >1000 Rt 202, PO Box 300, Raritan, NJ 08869 >phone 908-704-4295, fax 908-526-6469 >email shadden@SPAMFOIL.prius.jnj.com =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Peter Kieselbach ... the look in his eye Pharmacopeia, Inc seemed to say to the sky CN5350 "how to amuse them today?" Princeton, NJ 08543 (609) 452-3788 (phone) (732) 422-0156 (fax) petek@SPAMFOIL.pharmacop.com =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Tom Glenn" Subject: Re: Lab-Robotics: Alternate sources of Beckman Coulter pipet tips? Date: Tue, 2 Feb 1999 09:47:51 -0500 Another good supplier for you is : Denville Scientific 800 453-0385 They carry tips for Beckman, Zymark machines as well as other specialty products for robotics. Tom Glenn Biotech Sales Company Metuchen, NJ --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Andy Zaayenga Subject: Automated Mobile Phase Preparation Date: Wed, 03 Feb 1999 00:48:39 -0500 Has anyone had any experience with automated systems for making up mobile phase? This would be a liquid handling system preparing liters of mobile phase based on a predefined menu. Andy Zaayenga TekCel Corporation Mailto:andy.zaayenga@SPAMFOIL.tekcel.com Web Site: http://tekcel.com --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: "Thomas J. Baiga" Subject: Re: Lab-Robotics: Automated Mobile Phase Preparation Date: Tue, 02 Feb 1999 23:10:19 -0800 At 12:48 AM 2/3/99 -0500, you wrote: >--------------------- >Lab-Robotics >--------------------- >Has anyone had any experience with automated systems for making >up mobile phase? This would be a liquid handling system preparing >liters of mobile phase based on a predefined menu. > >Andy Zaayenga >TekCel Corporation >Mailto:andy.zaayenga@SPAMFOIL.tekcel.com >Web Site: http://tekcel.com > > > Andy, Take a look at my last posting. We've gone through all this design challenges regarding this issue during our development of a parallel purification workstation as well as a high-speed solid-phase wash/cleave workstation. I'd be happy to discuss this with you. If you prefer email, there are a few things I need to know: (1) how many source system fluids, (2) source volume and container, (3) will TFA be one of the source fluids, (4) Final mixing volumes, (5) do you need to degas (He, Ar, or N2), (6) single of multiple mixing chambers, (7) flow rate ranges, (8) liquid handler interface, (9) do you need sensor packages (mass flow, level, temperature, etc.), (10) master or slave controller, (11) precision/accuracy requirements... there are a few more nice-haves, but I think you get the idea. If you need something right off the shelf there are a few interesting things you can try. Have you looked at the Waters prep-scale HPLC pumps? Its a quaternary solvent handling system that features a quad proportional solenoid valve which meters solvent mixtures. The pump has a dual piston drive where the solvents get mixed in the primary piston chamber then dispensed from the secondary piston chamber. Moderate to high flow rates, and it ties into the Waters Millenium software nicely (but integrating in their Powerline code should only be attempted by very skilled coders). The Shimadzu LC8A pumps are nice too, but you need to gang them together and use an in-line mixer (helical rod assembly). Personally, I like to built these systems from the ground up because you can really control all the design parameters and features. For example, we are designing a system for our sister company Scylla Chemical which is going to integrate into a multi-workstation purification system for small molecules and organometallic complexes. To save on solvent costs (>1000 compounds per day) they are going to use bulk solvent that might be ACS grade at best. To get HPLC grade, the mobile phase system is being integrated with a solvent purification system based on in-line multi-stage media columns. Each workstation with have a solvent substation pre-filled from the source drums kept in a secure part of the facility (fire codes here require this approach). This way the substations take a big load off the HPLC pumps which otherwise would have to pump solvent in from another room (too much dead volume space). This way they can run fast gradients all day (and night) and regenerate parallel columns off-line. ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii From: Brendan_J_Connolly@SPAMFOIL.sbphrd.com Subject: Multimek and Beckman Stackers Date: Wed, 3 Feb 1999 10:54:54 +0000 We are looking at the Multimek with the Beckman Stackers and would loke to know if anyone has them already and how good are they. Thanks Brendan --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: William Suits Subject: Re: Lab-Robotics: Automated Mobile Phase Preparation Date: Wed, 03 Feb 1999 08:00:50 -0500 Andy, The choice of high pressure mixing versus low pressure mixing is common for chromatographers, but may not be for lab-robotics. Low pressure mixing combines solvents as one would in the lab. High pressure mixing will not give true proportions under pressure due to the different compressabilities. The only disadvantage to low pressure mixing is that degassing would most likely be required for reliable operation. Every HPLC pump has some dead volume associated. That volume may vary from 100 microliters to 6000 microliters, that may have to be flushed before delivering true proportions. Lower deadvolume mixers and improved pump check valves are available from ASI. Most HPLC pumps work better with at least 500 psi back pressure Bill Suits Chromatography Connections, Inc. billsuits@SPAMFOIL.earthlink.net Andy Zaayenga wrote: > --------------------- > Lab-Robotics > --------------------- > Has anyone had any experience with automated systems for making > up mobile phase? This would be a liquid handling system preparing > liters of mobile phase based on a predefined menu. > > Andy Zaayenga > TekCel Corporation > Mailto:andy.zaayenga@SPAMFOIL.tekcel.com > Web Site: http://tekcel.com > --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: Kevin Olsen Subject: Lab-Robotics: Automated Mobile Phase Preparation Date: Wed, 03 Feb 1999 11:33:50 -0500 Andy, I do not know of an off-the-shelf HPLC mobile phase preparation system. However, if I may be permitted to suggest how a system might be built? We have had great success with SciLog* pumps. They can be configured to dispense the large volumes required in such an application. They also can be configured to operate either connected to a balance or calibrated to dispense a pre-set amount. All of the fittings are Teflon and solvent resistance has not been a problem. An entire HPLC mobile phase system could be built from a few pumps and a dedicated PC. The SciLog pumps are very reliable and when they do break down, can be repaired easily. I had mine up and running again after only 15 minutes. SciLog's customer service was terrific. All of my questions were answered politely and promptly. One issue that your original posting did not address was that of regulatory compliance. Will this system operate under the watchful eye of FDA or EPA? If so, it might be worth the extra money to buy a complete system from SciLog and let them deal with the issues of software and performance validation. Sincerely, Kevin Olsen 914-732-3392 *SciLog 14 Ellis Potter Court Madison, Wi, 53711-2478 800-955-1993 608-278-9482 --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: John Ringeling Subject: custom laminar flow hood Date: Wed, 03 Feb 1999 12:19:58 -0500 Hi all: I was wondering if anyone has experience with companies that make custom LAMINAR flow hoods?! Thanks a lot Greetings John Ringeling ________________________________ Ringeling@SPAMFOIL.mpi.com Millennium Pharmaceuticals Inc. Protein and Antibody Technology 40 Erie Street Cambridge, MA 02139 (617) 679 7000 X-626 Fax: (617) 374 7790 --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7BIT From: "Abhijit Mitra" Subject: Re: Lab-Robotics: custom laminar flow hood Date: Wed, 3 Feb 1999 13:59:16 EST5EDT Hi Everybody! I would also like to know about Laminar Flow hoods. Abhijit Mitra 718-862-7109 (Voice) 718-862-7814 (Fax) --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Dominic Joseph Subject: Re: Lab-Robotics: custom laminar flow hood Date: Wed, 03 Feb 1999 16:51:03 -0500 At 01:59 PM 2/3/99 EST5EDT, you wrote: >--------------------- >Lab-Robotics >--------------------- >Hi Everybody! > >I would also like to know about Laminar Flow hoods. > >Abhijit Mitra >718-862-7109 (Voice) >718-862-7814 (Fax) --------------------------------------------- Hi, Nuaire makes Laminar flow hoods. NUAIRE, INC. 2100 FERNBROOK LANE, PLYMOUTH, MN 55447-4722 (612) 553-1270 TOLL FREE: (800) 328-3352 They have a website, www.nuaire.com. I believe their Labgard series appears in the catagory -> Biological safety cabinets. Best regards > > > Dominic Joseph ---------------------------------------------------------------------------- --- Millennium Pharmaceuticals | Email: joseph@SPAMFOIL.mpi.com | Pager EMAIL PIN 096 6722 640 Memorial Drive | Phone: (617) 679-7473 | Pager VMAIL 800-693 1494 Cambridge, MA 02139 | Fax: (617) 621-3520 | ---------------------------------------------------------------------------- --- --=_X9022820412355328Y= Content-Type: TEXT/PLAIN; CHARSET=US-ASCII From: Jacques van den Broek 0412662290 Subject: Re: Lab-Robotics: 384-well polypropylene plates Date: Thu, 04 Feb 1999 13:48:09 +0000 (GMT) Falcon has a nice 384 polyprop. plate. Jacques van den Broek. --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "david allen" Subject: RE: Lab-Robotics: correct tare weights of vials Date: Thu, 4 Feb 1999 08:55:14 -0500 > A co-worker of mine is investigating the reproducibility of vial > tare weights. In her own experiments , she has found a > significant (compared > to our sample sizes) difference in tare weights of empty, labeled > and capped > vials. By adjusting the ambient humidity, she has seen reproducible weight > variations as great as 15 miliigrams. Since our samples are often 15 > milligrams or less, you can see that this is a significant problem for us. > She has been able to determine that the problem is either the label or the > cap. She will do further experiments some time soon to determine if one or > both of them are the culprit. > labels and caps (mainly seals) can contain cellulose and clay which will exchange moisture with the air. you might want to change to a synthetic label stock (stay away from thermal print stock for related reasons) with a permenant adhesive and use a cap with a polypropylene seal. you can still get some variation in weight with humidity (glass will adsorb moisture) but, if you pre-condition, you should return to consistant conditions and get weighings repeatable to 1-2 mg. da --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "david allen" Subject: RE: Lab-Robotics: Automated Mobile Phase Preparation Date: Thu, 4 Feb 1999 09:04:52 -0500 > --------------------- > Lab-Robotics > --------------------- > Has anyone had any experience with automated systems for making > up mobile phase? This would be a liquid handling system preparing > liters of mobile phase based on a predefined menu. > I'm in the process of putting a system together now. I'm trying to use a FMI dual head proportioning pump to feed ACN and TFA, on demand, into a mixing vessel with a helium sparge. I have a capacitance sensor to monitor level and trigger a timer to run the pumps. the main problems are that this looks like some Goldberg kludge and it takes up about 3' of bench space. da --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Mark Gedrych" Subject: RE: Lab-Robotics: correct tare weights of vials Date: Thu, 4 Feb 1999 10:32:06 -0500 Jennifer and Susan, The Automation Partnership provides many thousands of pre-tared barcode labeled vials to our Haystack customers, and we have experienced the phenomenon that you describe. We tare weigh our vials without the cap, because this eliminates one possible cause of variation. Another advantage of this is that you are able to change caps (which vary significantly in weight) as required. We have only ever experienced a variation of 1-3mg, and so I wonder whether the cap could be a major contributor to your larger variation. In our experience, the effect does appear to be dependent on ambient humidity rather than temperature alone. We believe the label and glue are varying, rather than the glass, but this is not scientifically proven. We think it is the photographic emulsion part of the label, rather than the plastic film or the glue, but we have no evidence to support this belief. You don't say what humidity range you have tested over, so your larger weight range may simply reflect your exposure to a wider humidity range. If the lab ambient conditions are stable, we have not seen a problem, and the actual weights do not vary with time. So, although it may not be practical, keeping all of your labs at a fixed humidity should solve the problem. A more practical method can be employed within our Haystack sample bank management systems, and I would be happy to discuss the details of this with you if you give me a call. Mark __________________________________________________________________ Mark Gedrych, Haystack Product Manager, The Automation Partnership 266 Main Street, Suite 17, Medfield, MA 02052, USA Tel: (508) 359 5800 Ext 23 Fax: (508) 359 9352 mailto:Mark.Gedrych@SPAMFOIL.us.autoprt.com http://www.autoprt.com --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Ernie Woods" Subject: Re: Lab-Robotics: custom laminar flow hood Date: Thu, 4 Feb 1999 12:01:17 -0500 Hi John, Check out this site for a list of names of manufacturer's of laminar flow hoods. http://www.devicelink.com/company98/category/EnvironmentalControl_Products_a nd_Services/LaminarFlow_Hoods_.html Clicking on the company name yields more information to review. If you want to discuss on phone, give me a call. Good Luck Ernie Woods Hudson Control Group. -----Original Message----- From: John Ringeling To: Lab-Robotics Mailing List Date: Wednesday, February 03, 1999 13:20 Subject: Lab-Robotics: custom laminar flow hood >--------------------- >Lab-Robotics >--------------------- >Hi all: > >I was wondering if anyone has experience with companies that make custom >LAMINAR flow hoods?! > >Thanks a lot > >Greetings > >John Ringeling >________________________________ >Ringeling@SPAMFOIL.mpi.com > >Millennium Pharmaceuticals Inc. >Protein and Antibody Technology >40 Erie Street >Cambridge, MA 02139 >(617) 679 7000 X-626 >Fax: (617) 374 7790 > > --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Neal Holtzman" Subject: Re: Lab-Robotics: 384-well polypropylene plates Date: Thu, 4 Feb 1999 19:26:36 -0500 Marsh Biomedical Products sells an excellent polypropylene 384 well plate with a "v" bottom. This plate is extremely rigid for robotics compatibility and also does have the SBS footprint. For more information, please call customer service at 800-445-2812 and request a sample of P384VB. Alternately, request the sample via e-mail at sales@SPAMFOIL.biomar.com. Neal T. Holtzman neal@SPAMFOIL.biomar.com Director of Marketing Marsh Biomedical Products, Inc. 565 Blossom Road Rochester, NY 14610 800-445-2812 716-654-4800 Fax: 716-654-4810 Visit our WEB Site at http://www.biomar.com >> --------------------- >> Lab-Robotics >> --------------------- >> Does anyone know of a manufacturer of polypropylene 384-well plates? >> Thanks. >> >> Gwen Wise >> DuPont Pharmaceuticals > --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "OBPW" Subject: Re: Lab-Robotics: Automated Mobile Phase Preparation Date: Thu, 4 Feb 1999 23:04:52 -0500 Dear Andy, Sounds like something we could do for you. We have built such automated systems in the past which included PC for interface and maintenance of formulations, storage and metering of components into 1 to 40 liter mixing vessels, mixer controls, scale feedback, pH adjustments and more. Please send me more details of your requirements or call at 516-922-3789. Patrick Gaillard Oyster Bay Pump Works, Inc. -----Original Message----- From: Andy Zaayenga To: Lab-Robotics Mailing List Date: Wednesday, February 03, 1999 1:30 AM Subject: Lab-Robotics: Automated Mobile Phase Preparation >--------------------- >Lab-Robotics >--------------------- >Has anyone had any experience with automated systems for making >up mobile phase? This would be a liquid handling system preparing >liters of mobile phase based on a predefined menu. > >Andy Zaayenga >TekCel Corporation >Mailto:andy.zaayenga@SPAMFOIL.tekcel.com >Web Site: http://tekcel.com > --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: Christian R Strom Subject: pH meter Date: Fri, 05 Feb 1999 07:50:46 -0500 I currently have a Zymark system and I would like to add pH measuring capability to it. Does anyone have any comments on using Zymark's module card verses using RS232 communications (reliability, functionality)? I will be measuring a very viscous material and would like to know of any "wash stations" for the pH probes that have worked (or not). Thanks, Chris --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii From: Steve_Fillers@SPAMFOIL.biogen.com Subject: Re: Lab-Robotics: plate washer Date: Fri, 5 Feb 1999 09:41:51 -0500 Jennifer, I spoke with representatives from Biotek at the LabAutomation99 meeting this week. There is some confusion about this service/repair issue. Biotek is very concerned and have promised to provide clarification to this forum. Stay tuned! Steve From: "Jennifer Lee" Subject: Re: Lab-Robotics: plate washer Date: Tue, 26 Jan 1999 07:29:26 PST Hi, We have a Biotek EL403 plate washer which is broken. Because Biotek doesn't make them anymore, they no longer service them either. Does anyone know anyone who can fix them? Thanks, Jennifer Lee --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: Keith Gang Subject: Re: Lab-Robotics: pH meter Date: Fri, 5 Feb 1999 16:25:39 -0500 (EST) Chris: InnovaSystems makes a pH probe measurement and wash station that washes the probe with DI water and then air dries it prior to insertion into a 50 cc centrifuge tube placed on the unit. The station is made of materials such as Delrin, so a cleaner other than water can be used. It requires one input signal to raise/lower the probe and sends several output signals indicating probe position and status. These signals are compatible with the Zymark power and event controller. It stores the probe in a tap water reservoir when not in use. More info is available from Walter Stridick, InnovaSystems, Inc. @SPAMFOIL. (609)486-0030. Good luck. Keith Gang > --------------------- > Lab-Robotics > --------------------- > I currently have a Zymark system and I would like to add pH > measuring capability to it. Does anyone have any comments on > using Zymark's module card verses using RS232 communications > (reliability, functionality)? I will be measuring a very viscous > material and would like to know of any "wash stations" for the pH > probes that have worked (or not). > > Thanks, > Chris > > --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854";x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit From: Richard A Dalterio Subject: automated thermodynamic solubility of small 1-2 mg samples Date: Mon, 08 Feb 1999 16:40:45 -0500 I realize this is a tall order but here goes. We need to put together a system that can generate water solubility information for small-mass drug discovery samples. My dept. has recently been tasked with generating this info for a large number of samples on an ongoing basis. Sample quantities are limited to 1-2 mg per sample. I would like to suggest an outline of what I envision as the automated procedure and maybe members of the group can lend suggestions as to what type of hardware would best get the job done. The solubility sample stock would most likely be provided in dmso or ethanol solution (1-2 mg/200 ul). A small increment of this stock (1-2 ul) is added to 1800 ul water (5-20 ug/ml, and assume this goes into solution). Mix, measure UV, obtain absorbance at longest lambda max to calculate UV-absorptivity for this particular compound. The remaining stock solution in dmso or ethanol (198-199 ul) is added to the 1801-1802 ul water, stirred or swirled for some length of time for equilibration, filtered, and the UV measured. If the absorbance at the lambda max is above 1.2, the solution must be diluted and UV remeasured. When absorbance is 1.2 or less, the concentration (solubility) is calculated, using previously measured absorptivity and the dilution factor. A blank must be run for each series because dmso absorption tails out above 300 nm in a 10% solution. The UV spectral files should be saved for subsequent review, as should a record of dilution steps. Thanks for any help you may be able to provide. Rich Dalterio Bristol-Myers Squibb dalterir@SPAMFOIL.bms.com --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: "Thomas J. Baiga" Subject: Re: Lab-Robotics: automated thermodynamic solubility of small1-2 mg samples Date: Mon, 08 Feb 1999 16:16:44 -0800 Dear Richard, Not too tall of an order. This sounds like a nice application for a TECAN Genesis. Get one with the ROMA arm for plate manipulation (for your sample prep). Integrate in their detector (SLT) and all that's left is the sample processing. Their engineering staff should have this type of sample prep (filtration and mixing) experience. The last component will be the software to handle your IF/THEN conditions. Unfortunately this is not TECAN's strength. Maybe someone else out there has experience with Beckman's BioMek and would like to comment. Ultimately the important questions are: what throughput do you need/want, how flexible will the system need to be now/later and what's your budget. Good luck. Tom ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ --=_X9022820412355328Y= Content-Type: text/plain From: "Goering, Matt" Subject: Automating labels Date: Mon, 8 Feb 1999 16:45:23 -0800 Greetings everyone, I am looking for a way to automate the labeling of 4ml amber vials. Our labels need to be cold and solvent proof, with both barcodes and human readable text. Ideally we would be able to have both a strip with full details (compound name, solvent, molarity.....) and a small round dot for the cap that would hold just the compound name. It seems to me that getting something to label both the cap and the vial at the same time is the hard part. Currently we use an Intermec 3240 printer and apply the labels by hand. I have seen systems for applying labels to microtiter and deepwell plates, but not yet for vials. Presumably a system is already in place for this sort of thing. I'd appreciate any insights. Thanks Matt Goering Ligand Pharmaceuticals --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Rodney Stockton" Subject: Re: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg samples Date: Mon, 8 Feb 1999 18:14:04 -0800 Rich, This is actually a reasonably straight forward automation problem. The greatest difficulty will be in delivering the samples with the accuracies and precision at the quantities that you are requesting (1-2 uL). For example (and this is an exaggeration), if it was determined by weight (microbalance) that 4 uL was added instead of the required 2 uL would it be permissible to double the diluent volume and continue the analysis. Standard syringe pumps with calibrated syringes could perform the needed tasks if your actual volumes could vary a little from your target as long as you know exactly how much you have. The determination of the spectra and resulting dilutions based upon results is easy enough with the output of the spectrometer (RS-232 or IEEE) being fed into the automation computer (or running both from the same computer ie. dde linkage). Samples would be fed into the spectrometer via flow through cells with a dmso rinse between samples. Mixing can be done by vortex mixer. Filtration should be by pressure instead of vacuum to avoid evaporation of the dmso. I would recommend a gantry type robot or an inexpensive articulated robot such as the Mitsubishi RV-M1. The syringe pump I would recommend looking at is the by Cavro. The stroke length has a resolution of 24,000 steps therefore a calibrated syringe has a "theoritical" accuracy of 2 ml/24,000 = 0.08 uL. Of course the "actual" accuracy is no where near this close due to variances in the syringe, tubing to the tip, air displacement changes, etc. However it should be fairly easy to design a system that reliably delivers approximately 1 uL. Another possiblity is a digital pipette with disposable tips controlled via RS232. Please don't hesitate to contact me if you have any questions or if I can provide additional information for you. Rodney Stockton SLR Systems 2600D N.E. Stapleton Vancouver, WA 98661 360 693-3312 360 693-3323 fax rodney@SPAMFOIL.slrsystems.com Original Message----- From: Richard A Dalterio To: Lab-Robotics Mailing List Date: Monday, February 08, 1999 3:50 PM Subject: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg samples >--------------------- >Lab-Robotics >--------------------- >I realize this is a tall order but here goes. > >We need to put together a system that can generate water solubility >information for small-mass drug discovery samples. My dept. has >recently been tasked with generating this info for a large number >of samples on an ongoing basis. Sample quantities are limited to >1-2 mg per sample. I would like to suggest an outline of what I >envision as the automated procedure and maybe members of the group >can lend suggestions as to what type of hardware would best get the >job done. > >The solubility sample stock would most likely be provided in dmso or >ethanol solution (1-2 mg/200 ul). A small increment of this stock >(1-2 ul) is added to 1800 ul water (5-20 ug/ml, and assume this goes >into solution). Mix, measure UV, obtain absorbance at longest lambda >max to calculate UV-absorptivity for this particular compound. > >The remaining stock solution in dmso or ethanol (198-199 ul) is >added to the 1801-1802 ul water, stirred or swirled for some length >of time for equilibration, filtered, and the UV measured. If the >absorbance at the lambda max is above 1.2, the solution must be diluted >and UV remeasured. When absorbance is 1.2 or less, the concentration >(solubility) is calculated, using previously measured absorptivity and >the dilution factor. A blank must be run for each series because dmso >absorption tails out above 300 nm in a 10% solution. > >The UV spectral files should be saved for subsequent review, as >should a record of dilution steps. > >Thanks for any help you may be able to provide. > >Rich Dalterio >Bristol-Myers Squibb >dalterir@SPAMFOIL.bms.com > --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "David Allen" Subject: RE: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg samples Date: Mon, 8 Feb 1999 21:23:15 -0500 > We need to put together a system that can generate water solubility > information for small-mass drug discovery samples. My dept. has > recently been tasked with generating this info for a large number > of samples on an ongoing basis. Sample quantities are limited to > 1-2 mg per sample. I would like to suggest an outline of what I > envision as the automated procedure and maybe members of the group > can lend suggestions as to what type of hardware would best get the > job done. > just about any automation system could handle the task. you might contact Rich Kramer at the BMS facility in Princeton to see if he has any surplus systems (I've heard there may be some Benchmates floating around). da --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "David Allen" Subject: RE: Lab-Robotics: Automating labels Date: Mon, 8 Feb 1999 22:49:37 -0500 > Greetings everyone, > > I am looking for a way to automate the labeling of 4ml amber > vials. Our > labels need to be cold and solvent proof, with both barcodes and human > readable text. Ideally we would be able to have both a strip > with full > details (compound name, solvent, molarity.....) and a small round dot > for the cap that would hold just the compound name. It seems > to me that > getting something to label both the cap and the vial at the > same time is > the hard part. > one module to do both may be the hard part. you might check with Bohdan to see if they can put 2 label printers on one of their handlers. it'd be easier to print/apply the vial label then transport it to another unit which would read the label and print/apply a subset on a cap dot. > Currently we use an Intermec 3240 printer and apply the > labels by hand. > I have seen systems for applying labels to microtiter and deepwell > plates, but not yet for vials. Presumably a system is > already in place > for this sort of thing. I'd appreciate any insights. > if you have a transport unit (ie Zymark arm) you might look at Diagraph. they have an automatic print/apply that will actuate with a contact closure, but won't load/unload itself. da --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: David Subject: Re: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg samples Date: Tue, 09 Feb 1999 10:17:16 +0000 Richard A Dalterio wrote: > > --------------------- > Lab-Robotics > --------------------- > I realize this is a tall order but here goes. > > We need to put together a system that can generate water solubility > information for small-mass drug discovery samples. My dept. has Well I think this is something we could help with, along with a few others. We can quote for a complete robotic system if you wish. Please see our web page (below) Regards, David -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com BREAKFAST.COM Halted...Cereal Port Not Responding --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: "Whisenhunt, Donald W (CRD)" Subject: RE: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 07:29:54 -0500 Matt, We outsourced the labeling of our 2mL GC vials to Computype Inc. Drop me an email if you are interested in their number. We do not label the cap however. Donald Dr. Donald Whisenhunt Jr. General Electric Company Corporate Research and Development Combinatorial Chemistry Program PO Box 8 Bldg. K-1 5A24 Schenectady, NY 12301 518-387-5055 FAX 518-387-7611 Dial Comm 8*833-5055 Whisenhunt@SPAMFOIL.crd.ge.com > ---------- > From: Goering, Matt[SMTP:MGoering@SPAMFOIL.ligand.com] > Sent: Monday, February 08, 1999 7:45 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Automating labels > > --------------------- > Lab-Robotics > --------------------- > > Greetings everyone, > > I am looking for a way to automate the labeling of 4ml amber vials. Our > labels need to be cold and solvent proof, with both barcodes and human > readable text. Ideally we would be able to have both a strip with full > details (compound name, solvent, molarity.....) and a small round dot > for the cap that would hold just the compound name. It seems to me that > getting something to label both the cap and the vial at the same time is > the hard part. > > Currently we use an Intermec 3240 printer and apply the labels by hand. > I have seen systems for applying labels to microtiter and deepwell > plates, but not yet for vials. Presumably a system is already in place > for this sort of thing. I'd appreciate any insights. > > > Thanks > > Matt Goering > Ligand Pharmaceuticals > --=_X9022820412355328Y= Content-Type: text/plain From: "Shrago, Anne" Subject: RE: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 08:02:25 -0500 Sagian has a print and apply for 4 ml vials but only the vial is labeled. The labels are paper and would not hold up in cold storage. Perhaps a more compatible label can be customed for your application. The paper labels Sagian uses are custom from Weber. Anne Shrago GlaxoWellcome > -----Original Message----- > From: Goering, Matt [SMTP:MGoering@SPAMFOIL.ligand.com] > Sent: Monday, February 08, 1999 7:45 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Automating labels > > --------------------- > Lab-Robotics > --------------------- > > Greetings everyone, > > I am looking for a way to automate the labeling of 4ml amber vials. Our > labels need to be cold and solvent proof, with both barcodes and human > readable text. Ideally we would be able to have both a strip with full > details (compound name, solvent, molarity.....) and a small round dot > for the cap that would hold just the compound name. It seems to me that > getting something to label both the cap and the vial at the same time is > the hard part. > > Currently we use an Intermec 3240 printer and apply the labels by hand. > I have seen systems for applying labels to microtiter and deepwell > plates, but not yet for vials. Presumably a system is already in place > for this sort of thing. I'd appreciate any insights. > > > Thanks > > Matt Goering > Ligand Pharmaceuticals --=_X9022820412355328Y= Content-Type: text/plain From: "Werner, Chris" Subject: RE: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 08:12:27 -0500 Matt, I think a similar application was done by Bohdan Automation for a Big Pharma company. There was both a label on the circumference of the vial, and one that ran across the cap. I think the application ran pretty reliably after the kinks worked out. Most label issues regarding cold room and solvent compatibility have been dealt with successfully using synthetic label stock. Regards, Chris Werner Automated Systems Development ArQule, Inc. -----Original Message----- From: Goering, Matt [SMTP:MGoering@SPAMFOIL.ligand.com] Sent: Monday, February 08, 1999 7:45 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Automating labels --------------------- Lab-Robotics --------------------- Greetings everyone, I am looking for a way to automate the labeling of 4ml amber vials. Our labels need to be cold and solvent proof, with both barcodes and human readable text. Ideally we would be able to have both a strip with full details (compound name, solvent, molarity.....) and a small round dot for the cap that would hold just the compound name. It seems to me that getting something to label both the cap and the vial at the same time is the hard part. Currently we use an Intermec 3240 printer and apply the labels by hand. I have seen systems for applying labels to microtiter and deepwell plates, but not yet for vials. Presumably a system is already in place for this sort of thing. I'd appreciate any insights. Thanks Matt Goering Ligand Pharmaceuticals --=_X9022820412355328Y= Content-Type: text/plain From: Vladimir Pismenny Subject: RE: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 08:46:20 -0500 Matt, I know that Datavision installed similar system at American Medical Labs. Try Datavision's Bob Hogan at (301)-924-1886. Good luck, Vladimir > -----Original Message----- > From: Goering, Matt [SMTP:MGoering@SPAMFOIL.ligand.com] > Sent: Monday, February 08, 1999 7:45 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Automating labels > > --------------------- > Lab-Robotics > --------------------- > > Greetings everyone, > > I am looking for a way to automate the labeling of 4ml amber vials. > Our > labels need to be cold and solvent proof, with both barcodes and human > readable text. Ideally we would be able to have both a strip with > full > details (compound name, solvent, molarity.....) and a small round dot > for the cap that would hold just the compound name. It seems to me > that > getting something to label both the cap and the vial at the same time > is > the hard part. > > Currently we use an Intermec 3240 printer and apply the labels by > hand. > I have seen systems for applying labels to microtiter and deepwell > plates, but not yet for vials. Presumably a system is already in > place > for this sort of thing. I'd appreciate any insights. > > > Thanks > > Matt Goering > Ligand Pharmaceuticals --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: RFTRINKA Subject: Re: Lab-Robotics: Automating labels Date: Tue, 09 Feb 1999 09:58:26 -0800 Matt , Contact Tom Friedlander of Cyberlab. They have a very good system to label vials on the side. It uses the Intermec 3240 which you know is a very reliable label printer. They might be able to label the top also. You can investigate with them. They can send you a video. Tom's telephone # is 203-740-3565. Best Regards, Bob Trinka, Robocon US, Inc. > I am looking for a way to automate the labeling of 4ml amber vials. Our > labels need to be cold and solvent proof, with both barcodes and human > readable text. Ideally we would be able to have both a strip with full > details (compound name, solvent, molarity.....) and a small round dot > for the cap that would hold just the compound name. It seems to me that > getting something to label both the cap and the vial at the same time is > the hard part. > > Currently we use an Intermec 3240 printer and apply the labels by hand. > I have seen systems for applying labels to microtiter and deepwell > plates, but not yet for vials. Presumably a system is already in place > for this sort of thing. I'd appreciate any insights. > > Thanks > > Matt Goering > Ligand Pharmaceuticals --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: petek@SPAMFOIL.pharmacop.com (Kieselbach, Pete) Subject: Re: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 08:14:03 -0500 Matt: Biomedical Devices Co. showed something like this at Lab Automation a couple of years ago. They were showing print and apply systems for both vials and plates, though I don't think they were labeling the cap. They also had literature on tube labelers which used pre-printed stock. I think their email is: bdc@SPAMFOIL.bdc-automates.com (they don't seem to have a website) and the phone was 810/335-3232. Pete >--------------------- >Lab-Robotics >--------------------- > >Greetings everyone, > >I am looking for a way to automate the labeling of 4ml amber vials. Our >labels need to be cold and solvent proof, with both barcodes and human >readable text. Ideally we would be able to have both a strip with full >details (compound name, solvent, molarity.....) and a small round dot >for the cap that would hold just the compound name. It seems to me that >getting something to label both the cap and the vial at the same time is >the hard part. > >Currently we use an Intermec 3240 printer and apply the labels by hand. >I have seen systems for applying labels to microtiter and deepwell >plates, but not yet for vials. Presumably a system is already in place >for this sort of thing. I'd appreciate any insights. > > >Thanks > >Matt Goering >Ligand Pharmaceuticals =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Peter Kieselbach ... the look in his eye Pharmacopeia, Inc seemed to say to the sky CN5350 "how to amuse them today?" Princeton, NJ 08543 (609) 452-3788 (phone) (732) 422-0156 (fax) petek@SPAMFOIL.pharmacop.com =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit From: Sally Dowling Subject: RE: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 08:25:23 -0600 Matt: Bohdan Automation has installed workstation based automation to do exactly what you are requesting. We can share all the pertinent data and specifications with you and determine if any customization for your specific situation is required. We base our system on Zebra labellers and our X-Y-Z tabletop workstation. Your specific incoming and outgoing racks can be used. For example, we have implemented this label around the vial and dot on the cap for cryovials from a purification station, capping them as well as labelling, and placing them in freezer boxes for storage. Sally Dowling SDowling@SPAMFOIL.Bohdan.com >---------- >From: Goering, Matt[SMTP:MGoering@SPAMFOIL.ligand.com] >Sent: Monday, February 08, 1999 6:45 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics: Automating labels > >--------------------- >Lab-Robotics >--------------------- > >Greetings everyone, > >I am looking for a way to automate the labeling of 4ml amber vials. Our >labels need to be cold and solvent proof, with both barcodes and human >readable text. Ideally we would be able to have both a strip with full >details (compound name, solvent, molarity.....) and a small round dot >for the cap that would hold just the compound name. It seems to me that >getting something to label both the cap and the vial at the same time is >the hard part. > >Currently we use an Intermec 3240 printer and apply the labels by hand. >I have seen systems for applying labels to microtiter and deepwell >plates, but not yet for vials. Presumably a system is already in place >for this sort of thing. I'd appreciate any insights. > > >Thanks > >Matt Goering >Ligand Pharmaceuticals > > --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit From: Sally Dowling Subject: RE: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg samples Date: Tue, 9 Feb 1999 08:44:52 -0600 Richard: This is the type of application that sounded formidable in the days of robotic systems and big integration projects. Good news for you-- customizable workstations have been designed and installed by Bohdan Automation for just what you need. Dilution, filtration, spectroscopy, and data handling... all in a benchtop workstation platform. We'll gladly share details with you. As others have replied, the accuracy and precision are gained with stepper motor syringe drives and filtration is accomplished by attaching disc style filters in various sizes to use positive pressure to filter. You can choose your preferred UV. Jane Riley will be calling to discuss details. Best Regards, Sally Dowling SDowling@SPAMFOIL.Bohdan.com >---------- >From: Richard A Dalterio[SMTP:dalterir@SPAMFOIL.bms.com] >Sent: Monday, February 08, 1999 3:40 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg >samples > >--------------------- >Lab-Robotics >--------------------- >I realize this is a tall order but here goes. > >We need to put together a system that can generate water solubility >information for small-mass drug discovery samples. My dept. has >recently been tasked with generating this info for a large number >of samples on an ongoing basis. Sample quantities are limited to >1-2 mg per sample. I would like to suggest an outline of what I >envision as the automated procedure and maybe members of the group >can lend suggestions as to what type of hardware would best get the >job done. > >The solubility sample stock would most likely be provided in dmso or >ethanol solution (1-2 mg/200 ul). A small increment of this stock >(1-2 ul) is added to 1800 ul water (5-20 ug/ml, and assume this goes >into solution). Mix, measure UV, obtain absorbance at longest lambda >max to calculate UV-absorptivity for this particular compound. > >The remaining stock solution in dmso or ethanol (198-199 ul) is >added to the 1801-1802 ul water, stirred or swirled for some length >of time for equilibration, filtered, and the UV measured. If the >absorbance at the lambda max is above 1.2, the solution must be diluted >and UV remeasured. When absorbance is 1.2 or less, the concentration >(solubility) is calculated, using previously measured absorptivity and >the dilution factor. A blank must be run for each series because dmso >absorption tails out above 300 nm in a 10% solution. > >The UV spectral files should be saved for subsequent review, as >should a record of dilution steps. > >Thanks for any help you may be able to provide. > >Rich Dalterio >Bristol-Myers Squibb >dalterir@SPAMFOIL.bms.com > > --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit From: Mark Staebell Subject: Re: Lab-Robotics: Automating labels Date: Tue, 09 Feb 1999 13:53:17 -0700 Hi Matt, I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake City and, since I don't get out to lab automation shows very often, I am unaware of the companies providing solutions for putting barcodes on microtiter plates. Could you please forward any info you might have regarding the companies I should contact for these solutions? Any help you could provide would be appreciated. Sincerely, Mark Staebell --=_X9022820412355328Y= Content-Type: multipart/mixed; boundary="IMA.Boundary.412765819" From: DUNCAN.MCNICOL@SPAMFOIL.spcorp.com (DUNCAN MCNICOL) Subject: Re: Lab-Robotics: Automating labels Date: Tue, 9 Feb 1999 08:16:03 -0500 --IMA.Boundary.412765819 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Description: cc:Mail note part Matt, Bohdan Automation(a Mettler-Toledo company) makes automated labellers for tubes and vials. You should check with them to see if they can handle the size of vial you require. As far as the dot label on top... Maybe they might be able to help you there as well. ______________________________ Reply Separator _________________________________ Subject: Lab-Robotics: Automating labels Author: discussion@SPAMFOIL.lab-robotics.org at INTERNET Date: 2/8/99 4:45 PM --------------------- Lab-Robotics --------------------- Greetings everyone, I am looking for a way to automate the labeling of 4ml amber vials. Our labels need to be cold and solvent proof, with both barcodes and human readable text. Ideally we would be able to have both a strip with full details (compound name, solvent, molarity.....) and a small round dot for the cap that would hold just the compound name. It seems to me that getting something to label both the cap and the vial at the same time is the hard part. Currently we use an Intermec 3240 printer and apply the labels by hand. I have seen systems for applying labels to microtiter and deepwell plates, but not yet for vials. Presumably a system is already in place for this sort of thing. I'd appreciate any insights. Thanks Matt Goering Ligand Pharmaceuticals --IMA.Boundary.412765819 Content-Type: text/plain; charset=US-ASCII; name="RFC822 message headers" Content-Transfer-Encoding: 7bit Content-Description: cc:Mail note part Content-Disposition: inline; filename="RFC822 message headers" Received: from ans2.spri.sp.com (198.16.8.3) by smtpgw.inet.spri.sp.com with SMTP (IMA Internet Exchange 2.12 Enterprise) id 0011BB4E; Mon, 8 Feb 99 22:28:25 -0500 Received: from dfw-ix10.ix.netcom.com by sp2.spcorp.com with SMTP id WAA14636 (InterLock SMTP Gateway 3.0 for ); Mon, 8 Feb 1999 22:28:33 -0500 Received: (from smap@SPAMFOIL.localhost) by dfw-ix10.ix.netcom.com (8.8.4/8.8.4) id VAA26558; Mon, 8 Feb 1999 21:22:33 -0600 (CST) Message-Id: <199902090322.VAA26558@SPAMFOIL.dfw-ix10.ix.netcom.com> Received: from nbw-nj11-04.ix.netcom.com(207.94.119.132) by dfw-ix10.ix.netcom.com via smap (V1.3) id rma024632; Mon Feb 8 21:21:50 1999 From: "Goering, Matt" To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) Subject: Lab-Robotics: Automating labels Date: Mon, 8 Feb 1999 16:45:23 -0800 X-Priority: 3 Mime-Version: 1.0 Content-Type: text/plain Reply-To: discussion@SPAMFOIL.lab-robotics.org Sender: discussion@SPAMFOIL.lab-robotics.org --IMA.Boundary.412765819-- --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: "Thomas J. Baiga" Subject: Re: Lab-Robotics: automated thermodynamic solubility of small1-2 mg samples Date: Tue, 09 Feb 1999 20:21:01 -0800 At 08:44 AM 2/9/99 -0600, you wrote: >--------------------- >Lab-Robotics >--------------------- >Richard: > >This is the type of application that sounded formidable in the days of >robotic systems and big integration projects. > >Good news for you-- customizable workstations have been designed and >installed by Bohdan Automation for just what you need. > >Dilution, filtration, spectroscopy, and data handling... all in a >benchtop workstation platform. We'll gladly share details with you. As >others have replied, the accuracy and precision are gained with stepper >motor syringe drives and filtration is accomplished by attaching disc >style filters in various sizes to use positive pressure to filter. You >can choose your preferred UV. > >Jane Riley will be calling to discuss details. > >Best Regards, >Sally Dowling >SDowling@SPAMFOIL.Bohdan.com > >>---------- >>From: Richard A Dalterio[SMTP:dalterir@SPAMFOIL.bms.com] >>Sent: Monday, February 08, 1999 3:40 PM >>To: discussion@SPAMFOIL.lab-robotics.org >>Subject: Lab-Robotics: automated thermodynamic solubility of small 1-2 mg >>samples >> >>--------------------- >>Lab-Robotics >>--------------------- >>I realize this is a tall order but here goes. >> >>We need to put together a system that can generate water solubility >>information for small-mass drug discovery samples. My dept. has >>recently been tasked with generating this info for a large number >>of samples on an ongoing basis. Sample quantities are limited to >>1-2 mg per sample. I would like to suggest an outline of what I >>envision as the automated procedure and maybe members of the group >>can lend suggestions as to what type of hardware would best get the >>job done. >> >>The solubility sample stock would most likely be provided in dmso or >>ethanol solution (1-2 mg/200 ul). A small increment of this stock >>(1-2 ul) is added to 1800 ul water (5-20 ug/ml, and assume this goes >>into solution). Mix, measure UV, obtain absorbance at longest lambda >>max to calculate UV-absorptivity for this particular compound. >> >>The remaining stock solution in dmso or ethanol (198-199 ul) is >>added to the 1801-1802 ul water, stirred or swirled for some length >>of time for equilibration, filtered, and the UV measured. If the >>absorbance at the lambda max is above 1.2, the solution must be diluted >>and UV remeasured. When absorbance is 1.2 or less, the concentration >>(solubility) is calculated, using previously measured absorptivity and >>the dilution factor. A blank must be run for each series because dmso >>absorption tails out above 300 nm in a 10% solution. >> >>The UV spectral files should be saved for subsequent review, as >>should a record of dilution steps. >> >>Thanks for any help you may be able to provide. >> >>Rich Dalterio >>Bristol-Myers Squibb >>dalterir@SPAMFOIL.bms.com >> >> > Dear Rich, My apologies on the previous posting (especially to Sally), I forgot all about the various custom systems that Bohdan has done before. Sally, to your company's credit, you might suggest listing the various (and very interesting) systems that Bohdan has successfully developed and delivered over the years on your web site. I seem to recall hearing about a system that solves Rich's problem somewhere else (perhaps Sally can provide you with a reference). BEst Regards, Tom ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Labman Automation Subject: Lab-Robotics: correct tare weights of vials Date: Wed, 10 Feb 1999 09:09:48 -0500 VIAL TARE WEIGHING Labman Automation has had a lot of experience with a range of vials and balances with regards to automated weighing. The issues are, as always a little more complex than one would hope. The variables are; Humidity Temperature Air Pressure The affected elements are; Caps Labels and label gum Glass The compound Balance mechanics The two most significant aspects are moisture ingress into caps and labels and slow balance drift due to temperature and air pressure. Balance manufacturers quote repeatability but this is measured by a set of readings taken together and not with reasonable time intervals. One other factor we came across is the absorption of moisture by the compound once the cap was closed but this was with very hydroscopic mining samples. One difficulty is that we (Labman) live in a temperate climate and the relative humidity does not vary much from 54%. One interesting factor is the very sudden humidity change when snow starts to fall. (Like now!) The solution to these problems lies is minimising everything and with automated monitoring. A list might be as follows (depending on where you are and what 'elements' you have); 1. Small labels of the right material and gum 2. Weighing with the caps off 3. Automated balance calibration 4. Automated regular comparison with a check vial, the weight of which is known and is of the same type 5. Controlled lab conditions 6. Lots of time for vials to acclimatise 7. A balance that varies minimally with temperature change 8. At least a spare decimal point on the balance I hope that all helps. If you've any further questions please do not hesitate to ask. Best regards Andrew Whitwell. --=_X9022820412355328Y= Content-Type: text/plain From: "Goering, Matt" Subject: RE: Lab-Robotics: Automating labels Date: Wed, 10 Feb 1999 08:02:46 -0800 Mark, We apply our labels manually also, but I saw a Zymark system(Presto) that looked quite good. Bohdan also has labelling work stations for vials, I presume they have one for plates as well. If you talk to your printer manufacturer (ours is Intermec) they probably know who has integrated their printers into label automation as well. Matt > -----Original Message----- > From: Mark Staebell [SMTP:mstaebell@SPAMFOIL.myriad.com] > Sent: Tuesday, February 09, 1999 12:53 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Automating labels > > --------------------- > Lab-Robotics > --------------------- > Hi Matt, > > I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake > City and, since I don't get out to lab automation shows very often, I > am > unaware of the companies providing solutions for putting barcodes on > microtiter plates. Could you please forward any info you might have > regarding the companies I should contact for these solutions? > > Any help you could provide would be appreciated. > > Sincerely, > > Mark Staebell --=_X9022820412355328Y= Content-Type: text/plain From: Vladimir Pismenny Subject: RE: Lab-Robotics: Automating labels Date: Wed, 10 Feb 1999 11:41:58 -0500 Mark, DYNEX sells many types of microplates with pre-applied bar code labels. Labels symbology and location on the plate is done according to customer's specification. Contact: Mary Myers at 800-336-4543 ext 1225. Vladimir Pismenny > -----Original Message----- > From: Mark Staebell [SMTP:mstaebell@SPAMFOIL.myriad.com] > Sent: Tuesday, February 09, 1999 3:53 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Automating labels > > --------------------- > Lab-Robotics > --------------------- > Hi Matt, > > I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake > City and, since I don't get out to lab automation shows very often, I > am unaware of the companies providing solutions for putting barcodes on > microtiter plates. Could you please forward any info you might have > regarding the companies I should contact for these solutions? > > Any help you could provide would be appreciated. > > Sincerely, > > Mark Staebell --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" From: Teresa Ferragamo Subject: RE: Lab-Robotics: Automating labels Date: Wed, 10 Feb 1999 18:04:15 -0500 Hi Mark, I am the Applications Marketing person for Zymark's Presto(TM) product line. Below is an excerpt from a press release announcing the introduction of the Presto Microplate Labeler early last year. Zymark makes the Presto Microplate Labeler, a fast, reliable workstation for printing and applying adhesive barcode labels to microplates. This workstation is available as either a stand-alone workstation, integrated into a robotic system, or loaded using the Twister Microplate Handler. It is ideal for positive identification of plates stored in compound libraries and for plate replication. The Presto Labeler is a thermal transfer printer which uses high quality, solvent resistant label stock. True one-to-one barcode printing eliminates mismatched plates and labels. Teresa Ferragamo Zymark Corporation Applications Marketing Secretary, Society for Biomolecular Screening teresa.ferragamo@SPAMFOIL.zymark.com -----Original Message----- From: Mark Staebell [mailto:mstaebell@SPAMFOIL.myriad.com] Sent: Tuesday, February 09, 1999 3:53 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Automating labels --------------------- Lab-Robotics --------------------- Hi Matt, I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake City and, since I don't get out to lab automation shows very often, I am unaware of the companies providing solutions for putting barcodes on microtiter plates. Could you please forward any info you might have regarding the companies I should contact for these solutions? Any help you could provide would be appreciated. Sincerely, Mark Staebell --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Andy Zaayenga Subject: Re: Lab-Robotics: Automating labels Date: Wed, 10 Feb 1999 22:11:04 -0500 Matt, I worked with a customer in Sweden last year that is using Radio Frequency Identification (RFID) for 4 ml vial tracking. RFID is a neat technology because it does not require physical labeling - sample data is written and read electronically. The transponders can be passive (no battery) and are read/written by a RS232 linked 125 kHz tabletop unit. No physical contact takes place for read/write. You just move the vial cap close to the control head. The control head couples the power inductively for the data change in the transponder. The transponders are variable in size according to your needs for memory capacity and functionality. The transponders for the 4 ml vials fit neatly into the cap. They look like a small button. Think about the possibilities. You can dynamically record compound name, solvent, molarity, weight tracking, age, number and amounts of withdrawals, and so on. If the RFID system is propagated throughout your operation, you could write data to mother/daughter/granddaughter plates based on the vial information and system operations. Some of the issues of paper labels changing weight with environment would go away. Static barcode information does not have to be written on labels and applied to the vials. The down side - no human readable information is visually available. You could have a control head for displaying the data but manually searching for a specific vial out of many would be very difficult. It's a great technology that has little penetration into the laboratory automation field as yet. We should see more of it as less manual intervention is required in our processes. If and when automation shifts to a compound vial in and data out model, this type of tracking becomes very attractive. Has anyone on this Discussion List worked with RFID? Some good RFID sites: http://www.cwt.vt.edu/faq/rfid.htm http://rapidttp.com/transponder/ http://www.microncommunications.com/ By the way, I did work with a conventional vial labeller a couple of years ago. It was a Willet unit and sprayed the barcode information directly on the vial without a label. It was difficult to set up but worked fairly well after that. Perhaps a solution is RFID for the cap and conventional barcoding for the vial. Regards, Andy Zaayenga TekCel Corporation Mailto:andy.zaayenga@SPAMFOIL.tekcel.com Web Site: http://tekcel.com At 04:45 PM 2/8/99 -0800, Goering, Matt wrote to Andy: |--------------------- |Lab-Robotics |--------------------- | |Greetings everyone, | |I am looking for a way to automate the labeling of 4ml amber vials. Our |labels need to be cold and solvent proof, with both barcodes and human |readable text. Ideally we would be able to have both a strip with full |details (compound name, solvent, molarity.....) and a small round dot |for the cap that would hold just the compound name. It seems to me that |getting something to label both the cap and the vial at the same time is |the hard part. | |Currently we use an Intermec 3240 printer and apply the labels by hand. |I have seen systems for applying labels to microtiter and deepwell |plates, but not yet for vials. Presumably a system is already in place |for this sort of thing. I'd appreciate any insights. | | |Thanks | |Matt Goering |Ligand Pharmaceuticals | | --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "David Allen" Subject: RE: Lab-Robotics: Automating labels Date: Wed, 10 Feb 1999 23:05:39 -0500 > I worked with a customer in Sweden last year that is using > Radio Frequency Identification (RFID) for 4 ml vial tracking. the IRORI system uses the same concept. they use a glass enclosed transponder about the size of a very small stir bar. there's a stand alone reader that will plug into a PC and software for tracking the chips. the system's a little pricy for single use applications ($8 per chip plus several thou for reader and software), but if you need harsh environment/automated ID it fills the bill. > model, this type of tracking becomes very attractive. Has > anyone on this Discussion List worked with RFID? > we're using their system to track reactions. one chip per reaction vial and we can track what's been done and needs doing. human readable info is still a problem, but their reader and sorting software helps. one alternative we've been looking at is using a CNC engraver to etch info directly on the containers. it takes a lot longer than a printer (2 to 5 min/vial), but we save a lot of time during manual handling. if we could find a cheap and fast vision system that would read the etched characters, we might be able to accept a slow engraving time. da --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Charlie d'Estries" Subject: asking for your help and opinion Date: Thu, 11 Feb 1999 10:05:22 -0500 Hi folks, I'm wondering if someone could help me with the proper protocol here. I'm conducting a survey for an HTS manufacturer who is collecting data on designing a new instrument system, but looking for accurate answers to some technical questions. Is there a way I can ask people in this forum for their opinions properly, without spamming? All opinions are appreciated. Thanks for your help. Charlie d'Estries Independent Consultant SciBiz International 716-662-4121 Phone 716-662-4175 Fax scibiz@SPAMFOIL.buffnet.net Need some help in getting your product to market? Looking for a speaker at your next sales meeting? My expertise is in market research, sales & distribution of technology based products and services. --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: chance@SPAMFOIL.molecumetics.com (Chance Elliott) Subject: MPII MSL COMMANDS Date: Thu, 11 Feb 1999 09:06:37 -0800 I am looking for some example MSL script for the Multiprobe II. There are two things I am trying to do 1) Open a name pipe as a pre step function. 2) Open and sort the nameoftest_labware.csv as a pre step function. I have some example MSL stuff that is included in winprep but there is not a whole lot there and there doesn't seem to be any other documentation on MSL anywhere. If anyone could help me out I would really appreciate it. Thanks! Chance Elliott Research Automation Engineer Molecumetics LTD --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: songzhu an Subject: luminometer Date: Thu, 11 Feb 1999 09:43:50 -0800 Hi, HTS folks: I'm looking for a luminometer for reporter gene HTS. Which company, in your opinion, makes the best luminometer for HTS of reporter gene? Is there such a luminometer that read 384 wells simultaneously like a FLIPR does in fluorescence. I heard of LuFLIPRase. Does anyone know the status of that? I'd very much appreciate your help. Thanks! Songzhu ******************************** Songzhu An, M.D., Ph.D. Assistant Professor Department of Medicine University of California Mail Box 0711, Rm Ub-8 San Francisco, CA 94143 Tel.: (415)476-1531 Fax: (415)476-6915 Email: songzhu@SPAMFOIL.itsa.ucsf.edu ******************************** --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Rodney Stockton" Subject: Re: Lab-Robotics: Automating labels Date: Thu, 11 Feb 1999 10:53:42 -0800 Just a sidelight note for the RF Identification technique. It has been used successfully here in the northwest to track tagged salmon throught the fish ladders of the dams, monitor the movement of dairy cows around feeders and milking stalls, monitor the movement of silicon wafer tubs through the manufacturing process. So it is actually a viable technology. Sorry for the digression, just one of those useless tidbits of info. Rodney Stockton SLR Systems 2600D N.E. Stapleton Vancouver, WA 98661 360 693-3312 360 693-3323 fax rodney@SPAMFOIL.slrsystems.com -----Original Message----- From: Andy Zaayenga To: Lab-Robotics Mailing List Date: Wednesday, February 10, 1999 7:35 PM Subject: Lab-Robotics: Automating labels >--------------------- >Lab-Robotics >--------------------- >Matt, > >I worked with a customer in Sweden last year that is using Radio Frequency Identification (RFID) for 4 ml vial tracking. RFID is a neat technology because it does not require physical labeling - sample data is written and read electronically. The transponders can be passive (no battery) and are read/written by a RS232 linked 125 kHz tabletop unit. No physical contact takes place for read/write. You just move the vial cap close to the control head. The control head couples the power inductively for the data change in the transponder. The transponders are variable in size according to your needs for memory capacity and functionality. The transponders for the 4 ml vials fit neatly into the cap. They look like a small button. > >Think about the possibilities. You can dynamically record compound name, solvent, molarity, weight tracking, age, number and amounts of withdrawals, and so on. If the RFID system is propagated throughout your operation, you could write data to mother/daughter/granddaughter plates based on the vial information and system operations. Some of the issues of paper labels changing weight with environment would go away. Static barcode information does not have to be written on labels and applied to the vials. > >The down side - no human readable information is visually available. You could have a control head for displaying the data but manually searching for a specific vial out of many would be very difficult. > >It's a great technology that has little penetration into the laboratory automation field as yet. We should see more of it as less manual intervention is required in our processes. If and when automation shifts to a compound vial in and data out model, this type of tracking becomes very attractive. Has anyone on this Discussion List worked with RFID? > >Some good RFID sites: >http://www.cwt.vt.edu/faq/rfid.htm >http://rapidttp.com/transponder/ >http://www.microncommunications.com/ > >By the way, I did work with a conventional vial labeller a couple of years ago. It was a Willet unit and sprayed the barcode information directly on the vial without a label. It was difficult to set up but worked fairly well after that. Perhaps a solution is RFID for the cap and conventional barcoding for the vial. > >Regards, > >Andy Zaayenga >TekCel Corporation >Mailto:andy.zaayenga@SPAMFOIL.tekcel.com >Web Site: http://tekcel.com > > >At 04:45 PM 2/8/99 -0800, Goering, Matt wrote to Andy: >|--------------------- >|Lab-Robotics >|--------------------- >| >|Greetings everyone, >| >|I am looking for a way to automate the labeling of 4ml amber vials. Our >|labels need to be cold and solvent proof, with both barcodes and human >|readable text. Ideally we would be able to have both a strip with full >|details (compound name, solvent, molarity.....) and a small round dot >|for the cap that would hold just the compound name. It seems to me that >|getting something to label both the cap and the vial at the same time is >|the hard part. >| >|Currently we use an Intermec 3240 printer and apply the labels by hand. >|I have seen systems for applying labels to microtiter and deepwell >|plates, but not yet for vials. Presumably a system is already in place >|for this sort of thing. I'd appreciate any insights. >| >| >|Thanks >| >|Matt Goering >|Ligand Pharmaceuticals >| >| > --=_X9022820412355328Y= Content-Type: text/plain; charset="US-Ascii" Content-Transfer-Encoding: 7bit From: Julie Monagle Subject: RE: Lab-Robotics: Automating labels Date: 11 Feb 99 18:56:02 -0500 Mark, We have a Presto labeler and it has some limitations with the type of barcodes that you can create. To date we are having trouble getting any other characters on the labels other than alpha and or numeric, ie tab, _ etc. Julie Monagle Goering, Matt wrote: >--------------------- >Lab-Robotics >--------------------- >Mark, > >We apply our labels manually also, but I saw a Zymark system(Presto) >that looked quite good. Bohdan also has labelling work stations for >vials, I presume they have one for plates as well. If you talk to your >printer manufacturer (ours is Intermec) they probably know who has >integrated their printers into label automation as well. > >Matt > >> -----Original Message----- >> From: Mark Staebell [SMTP:mstaebell@SPAMFOIL.myriad.com] >> Sent: Tuesday, February 09, 1999 12:53 PM >> To: discussion@SPAMFOIL.lab-robotics.org >> Subject: Lab-Robotics: Automating labels >> >> --------------------- >> Lab-Robotics >> --------------------- >> Hi Matt, >> >> I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake >> City and, since I don't get out to lab automation shows very often, I >> am >> unaware of the companies providing solutions for putting barcodes on >> microtiter plates. Could you please forward any info you might have >> regarding the companies I should contact for these solutions? >> >> Any help you could provide would be appreciated. >> >> Sincerely, >> >> Mark Staebell > > > >RFC822 header >----------------------------------- > >RECEIVED: from SF_Database by POP_Mailbox_-1293473486 ; 10 FEB 99 12:16:50 >UT >Received: from DFW-IX13.IX.NETCOM.COM by nrgn.nrgn.com > with SMTP (QuickMail Pro Server for MacOS 1.1.1r1); 10 FEB 99 12:16:43 UT >Received: (from smap@SPAMFOIL.localhost) > by dfw-ix13.ix.netcom.com (8.8.4/8.8.4) > id KAA28332; Wed, 10 Feb 1999 10:33:08 -0600 (CST) >Message-Id: <199902101633.KAA28332@SPAMFOIL.dfw-ix13.ix.netcom.com> >Received: from nbw-nj11-32.ix.netcom.com(207.94.119.160) by >dfw-ix13.ix.netcom.com via smap (V1.3) > id rma028097; Wed Feb 10 10:32:19 1999 >From: "Goering, Matt" >To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) >Subject: Lab-Robotics: Automating labels >Date: Wed, 10 Feb 1999 08:02:46 -0800 >X-Priority: 3 >MIME-Version: 1.0 >Content-Type: text/plain >Reply-To: discussion@SPAMFOIL.lab-robotics.org >Sender: discussion@SPAMFOIL.lab-robotics.org > --=_X9022820412355328Y= Content-Type: text/plain; charset="US-Ascii" Content-Transfer-Encoding: 7bit From: Julie Monagle Subject: RE: Lab-Robotics: Automating labels Date: 11 Feb 99 18:56:06 -0500 We are currently having trouble with the labels being DMSO tolerant. At least, the stock that came with the Presto does not stand up to DMSO. Julie Monagle Teresa Ferragamo wrote: >--------------------- >Lab-Robotics >--------------------- >Hi Mark, > >I am the Applications Marketing person for Zymark's Presto(TM) product line. >Below is an excerpt from a press release announcing the introduction of the >Presto Microplate Labeler early last year. > >Zymark makes the Presto Microplate Labeler, a fast, reliable workstation for >printing and applying adhesive barcode labels to microplates. This >workstation is available as either a stand-alone workstation, integrated >into a robotic system, or loaded using the Twister Microplate Handler. > >It is ideal for positive identification of plates stored in compound >libraries and for plate replication. The Presto Labeler is a thermal >transfer printer which uses high quality, solvent resistant label stock. >True one-to-one barcode printing eliminates mismatched plates and labels. > >Teresa Ferragamo >Zymark Corporation >Applications Marketing >Secretary, Society for Biomolecular Screening >teresa.ferragamo@SPAMFOIL.zymark.com > > > >-----Original Message----- >From: Mark Staebell [mailto:mstaebell@SPAMFOIL.myriad.com] >Sent: Tuesday, February 09, 1999 3:53 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics: Automating labels > > >--------------------- >Lab-Robotics >--------------------- >Hi Matt, > >I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake >City and, since I don't get out to lab automation shows very often, I am >unaware of the companies providing solutions for putting barcodes on >microtiter plates. Could you please forward any info you might have >regarding the companies I should contact for these solutions? > >Any help you could provide would be appreciated. > >Sincerely, > >Mark Staebell > > > >RFC822 header >----------------------------------- > >RECEIVED: from SF_Database by POP_Mailbox_-1293450227 ; 10 FEB 99 18:44:29 >UT >Received: from DFW-IX4.IX.NETCOM.COM by nrgn.nrgn.com > with SMTP (QuickMail Pro Server for MacOS 1.1.1r1); 10 FEB 99 18:44:21 UT >Received: (from smap@SPAMFOIL.localhost) > by dfw-ix4.ix.netcom.com (8.8.4/8.8.4) > id RAA18013; Wed, 10 Feb 1999 17:17:37 -0600 (CST) >Message-Id: <199902102317.RAA18013@SPAMFOIL.dfw-ix4.ix.netcom.com> >Received: from nbw-nj10-40.ix.netcom.com(207.94.119.104) by >dfw-ix4.ix.netcom.com via smap (V1.3) > id rma017825; Wed Feb 10 17:16:29 1999 >From: Teresa Ferragamo >To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) >Subject: Lab-Robotics: Automating labels >Date: Wed, 10 Feb 1999 18:04:15 -0500 >MIME-Version: 1.0 >Content-Type: text/plain; > charset="iso-8859-1" >Reply-To: discussion@SPAMFOIL.lab-robotics.org >Sender: discussion@SPAMFOIL.lab-robotics.org > --=_X9022820412355328Y= Content-Type: text/plain; CHARSET=US-ASCII From: "Ramesha, Chakk {RES~Palo Alto}" Subject: RE: Lab-Robotics: luminometer Date: Thu, 11 Feb 1999 16:35:35 -0800 Molecular Devises says that their FLIPR can read luciferase. We spent some time but without much luck. We use Packard Top count whcich is capable of reading 384-wells but not simultaneously. In addition this is a end point read. Chakk Ramesha High Throughput Screening IDU, Roche Bioscience Palo Alto, CA 94303 Tel: 650-855-5847 Fax: 650-354-7393 email: chakk.ramesha@SPAMFOIL.roche.com > -----Original Message----- > From: songzhu an [SMTP:songzhu@SPAMFOIL.itsa.ucsf.edu] > Sent: Thursday, February 11, 1999 9:44 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: luminometer > > --------------------- > Lab-Robotics > --------------------- > Hi, HTS folks: > > I'm looking for a luminometer for reporter gene HTS. Which company, in > your opinion, makes the best luminometer for HTS of reporter gene? Is > there such a luminometer that read 384 wells simultaneously like a FLIPR > does in fluorescence. I heard of LuFLIPRase. Does anyone know the status > of that? > > I'd very much appreciate your help. Thanks! > > Songzhu > > ******************************** > Songzhu An, M.D., Ph.D. > Assistant Professor > Department of Medicine > University of California > Mail Box 0711, Rm Ub-8 > San Francisco, CA 94143 > Tel.: (415)476-1531 > Fax: (415)476-6915 > Email: songzhu@SPAMFOIL.itsa.ucsf.edu > ******************************** --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: "Cesarek, John" (by way of Laboratory Robotics Interest Group ) Subject: Visual Basic Programs to link Packard MPII to network. Date: Thu, 11 Feb 1999 19:36:35 -0500 I looking for a Visual Basic program that can connect a Packard MPII to a network or internet. Does anyone know of any websites that specializes in lab automation add-on programs. Thanks, John Cesarek Mycogen/Dow AgroScience --=_X9022820412355328Y= Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit From: buglite@SPAMFOIL.icss.net (Jim Fairchild) Subject: Re: Lab-Robotics: luminometer Date: Thu, 11 Feb 1999 20:33:31 -0600 Hi Songzhu, Torcon's Assayer and Gold Digger can read a 384 well plate in 60 seconds. Four wells simultaneously and no crosstalk! Give them a peek at Jim Swansea, IL ---------- : From: Ramesha, Chakk {RES~Palo Alto} : To: Lab-Robotics Mailing List : Subject: Lab-Robotics: luminometer : Date: Thursday, February 11, 1999 6:35 PM : : --------------------- : Lab-Robotics : --------------------- : Molecular Devises says that their FLIPR can read luciferase. We spent some : time but without much luck. We use Packard Top count whcich is capable of : reading 384-wells but not simultaneously. In addition this is a end point : read. : : Chakk Ramesha : High Throughput Screening : IDU, Roche Bioscience : Palo Alto, CA 94303 : : Tel: 650-855-5847 : Fax: 650-354-7393 : email: chakk.ramesha@SPAMFOIL.roche.com : : : > -----Original Message----- : > From: songzhu an [SMTP:songzhu@SPAMFOIL.itsa.ucsf.edu] : > Sent: Thursday, February 11, 1999 9:44 AM : > To: discussion@SPAMFOIL.lab-robotics.org : > Subject: Lab-Robotics: luminometer : > : > --------------------- : > Lab-Robotics : > --------------------- : > Hi, HTS folks: : > : > I'm looking for a luminometer for reporter gene HTS. Which company, in : > your opinion, makes the best luminometer for HTS of reporter gene? Is : > there such a luminometer that read 384 wells simultaneously like a FLIPR : > does in fluorescence. I heard of LuFLIPRase. Does anyone know the status : > of that? : > : > I'd very much appreciate your help. Thanks! : > : > Songzhu : > : > ******************************** : > Songzhu An, M.D., Ph.D. : > Assistant Professor : > Department of Medicine : > University of California : > Mail Box 0711, Rm Ub-8 : > San Francisco, CA 94143 : > Tel.: (415)476-1531 : > Fax: (415)476-6915 : > Email: songzhu@SPAMFOIL.itsa.ucsf.edu : > ******************************** --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: "Ray Boutin" Subject: Re: Lab-Robotics: luminometer Date: Fri, 12 Feb 1999 05:47:13 -0500 Songzhu: Rosys-Anthos has a new integrated robotic system with luminometer called AutoLucy. We have had several installations already and the system looks good. The same concept, reader and software can also be transferred over to our larger systems. Please give me a call if you need more information, literature, etc. Ray Boutin Sales Engineer Rosys Anthos 302-326-0433 At 09:43 AM 2/11/99 -0800, you wrote: >--------------------- >Lab-Robotics >--------------------- >Hi, HTS folks: > >I'm looking for a luminometer for reporter gene HTS. Which company, in >your opinion, makes the best luminometer for HTS of reporter gene? Is >there such a luminometer that read 384 wells simultaneously like a FLIPR >does in fluorescence. I heard of LuFLIPRase. Does anyone know the status >of that? > >I'd very much appreciate your help. Thanks! > >Songzhu > >******************************** >Songzhu An, M.D., Ph.D. >Assistant Professor >Department of Medicine >University of California >Mail Box 0711, Rm Ub-8 >San Francisco, CA 94143 >Tel.: (415)476-1531 >Fax: (415)476-6915 >Email: songzhu@SPAMFOIL.itsa.ucsf.edu >******************************** > > --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: Martin M Echols Subject: Re: Lab-Robotics: Visual Basic Programs to link Packard MPII tonetwork. Date: Fri, 12 Feb 1999 08:24:29 -0500 Why the interest in a VB program vs some other language? Martin Cesarek, John (by way of Laboratory Robotics Interest Group ) wrote: > > --------------------- > Lab-Robotics > --------------------- > > I looking for a Visual Basic program that can connect a Packard > MPII to a network or internet. Does anyone know of any websites that > specializes in lab automation add-on programs. > > Thanks, > > John Cesarek > Mycogen/Dow AgroScience --=_X9022820412355328Y= Content-Type: TEXT/PLAIN; charset=US-ASCII From: "ANUJA.ANILKUMAR.SONALKER" Subject: Please help--ERP ! Date: Fri, 12 Feb 1999 21:37:17 +0500 (IST) Dear Mr Charlie, I am looking for a market research expert for some help. I am writing a paper on "Importance of ERP solutions to business" for which I need certain real data from a company having adopted ERP. The company should be either a pharmaceutical or a consumer goods company. I would like to get hold of their financial calculations for any one commodity before and after installing ERP. The data after ERP installation must show significant increase in profit or quicker and efficient generation of profit and workability than before. The financial calculations need not involve any secretive/ strategic data which you may not be comfortable releasing. Minimally, the following format: The datasheet/ balancesheet need not be the latest, if it satisfies all other conditions. Sheet1 Sr.no Description Qty Rate Fixed cost Variable cost Investment Labour Electricity Water Maintainance Extra Mis. Components: a) b) Octroi Freight Total: Sheet2 Units Total cost Revenues Rate/ Unit Variable Profit Kindly respond to me at the earliest at my address: onkars@SPAMFOIL.giasbma.vsnl.net.in Anuja Sonalker. --=_X9022820412355328Y= Content-Type: text/plain From: "McCook, Tony" Subject: RE: Lab-Robotics: luminometer Date: Fri, 12 Feb 1999 15:35:52 -0500 Dear Songzhu, EG & G Wallac Inc. sells three different choices for doing HTS reporter gene assays that can measure 384 well plates and have been integrated into robotic systems : the VICTOR2 multilabel reader, the MicroBeta Jet, the LB96V MicroLumat. All three come with the option for integrated injectors and the MicroBeta Jet and MicroLumat have been validated as DLR (Dual Luciferase Ready) by Promega. If you would like more infomation, please call 1-800-638-6692, visit our website: www.wallac.com or send us an email to . Sincerely, Tony McCook Marketing Manager EG & G Wallac Inc. 800-638-6692x207 > -----Original Message----- > From: songzhu an [SMTP:songzhu@SPAMFOIL.itsa.ucsf.edu] > Sent: Thursday, February 11, 1999 12:44 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: luminometer > > --------------------- > Lab-Robotics > --------------------- > Hi, HTS folks: > > I'm looking for a luminometer for reporter gene HTS. Which company, in > your opinion, makes the best luminometer for HTS of reporter gene? Is > there such a luminometer that read 384 wells simultaneously like a FLIPR > does in fluorescence. I heard of LuFLIPRase. Does anyone know the status > of that? > > I'd very much appreciate your help. Thanks! > > Songzhu > > ******************************** > Songzhu An, M.D., Ph.D. > Assistant Professor > Department of Medicine > University of California > Mail Box 0711, Rm Ub-8 > San Francisco, CA 94143 > Tel.: (415)476-1531 > Fax: (415)476-6915 > Email: songzhu@SPAMFOIL.itsa.ucsf.edu > ******************************** --=_X9022820412355328Y= Content-Type: text/plain From: "Cesarek, John" Subject: RE: Lab-Robotics: Visual Basic Programs to link Packard MPII network. Date: Fri, 12 Feb 1999 14:35:19 -0600 Hi Martin I'm really not sure what programs would work the best for this application. I was hoping that their might be some third party software that would help me out. Thanks, John > -----Original Message----- > From: Martin M Echols [SMTP:echolsm@SPAMFOIL.bms.com] > Sent: Friday, February 12, 1999 5:24 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: Visual Basic Programs to link Packard MPII > tonetwork. > > --------------------- > Lab-Robotics > --------------------- > Why the interest in a VB program vs some other language? > > Martin > > Cesarek, John (by way of Laboratory Robotics Interest Group ) wrote: > > > > --------------------- > > Lab-Robotics > > --------------------- > > > > I looking for a Visual Basic program that can connect a Packard > > MPII to a network or internet. Does anyone know of any websites > that > > specializes in lab automation add-on programs. > > > > Thanks, > > > > John Cesarek > > Mycogen/Dow AgroScience --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: John Elling Subject: Re: Lab-Robotics: Visual Basic Programs to link Packard MPIIto network. Date: Fri, 12 Feb 1999 14:38:40 -0700 Wiley has a book comming out on Visual Basic for automation of laboratory equipment (or some such title). I think I recall example code relevant to this problem in a preprint. You might get more information from the editor, Carla A. Fjerstad, CFiersta@SPAMFOIL.Wiley.com. At 07:36 PM 2/11/99 -0500, you wrote: >--------------------- >Lab-Robotics >--------------------- > >I looking for a Visual Basic program that can connect a Packard >MPII to a network or internet. Does anyone know of any websites that >specializes in lab automation add-on programs. > >Thanks, > >John Cesarek >Mycogen/Dow AgroScience > > > John W. Elling, Ph.D., MBA Bioreason, Inc. 309 Johnson Street Santa Fe, NM 87501 phone (505) 995-8188 fax (505) 995-8186 cell (505) 670-0232 elling@SPAMFOIL.bioreason.com www.bioreason.com --=_X9022820412355328Y= Content-Type: text/plain From: James LaRocque Subject: Re: Lab-Robotics: Automating labels Date: Tue, 16 Feb 1999 13:27:48 -0500 We have been using the Zymark Presto Labeler for many months to label polypropylene plates containing samples in 100% DMSO for storage at -80 C and for shipment on dry ice. We have had no problems with the labels or the printer but we typically do not get DMSO on the labels. We currently have one labeler integrated into a Zymark XP system running through "PCS" via a DDE on the PC and a second connected directly to the system V controller. The font the printer uses only supports alpha-numeric characters but you can use excel to manipulate your list so that tabs are not a problem. If you need things like a % or # sign, you are out of luck. Jim LaRocque From: Julie Monagle Subject: RE: Lab-Robotics: Automating labels Date: 11 Feb 99 18:56:06 -0500 We are currently having trouble with the labels being DMSO tolerant. At least, the stock that came with the Presto does not stand up to DMSO. Julie Monagle Teresa Ferragamo wrote: >--------------------- >Lab-Robotics >--------------------- >Hi Mark, > >I am the Applications Marketing person for Zymark's Presto(TM) product line. >Below is an excerpt from a press release announcing the introduction of the >Presto Microplate Labeler early last year. > >Zymark makes the Presto Microplate Labeler, a fast, reliable workstation for >printing and applying adhesive barcode labels to microplates. This >workstation is available as either a stand-alone workstation, integrated >into a robotic system, or loaded using the Twister Microplate Handler. > >It is ideal for positive identification of plates stored in compound >libraries and for plate replication. The Presto Labeler is a thermal >transfer printer which uses high quality, solvent resistant label stock. >True one-to-one barcode printing eliminates mismatched plates and labels. > >Teresa Ferragamo >Zymark Corporation >Applications Marketing >Secretary, Society for Biomolecular Screening >teresa.ferragamo@SPAMFOIL.zymark.com > > > >-----Original Message----- >From: Mark Staebell [mailto:mstaebell@SPAMFOIL.myriad.com] >Sent: Tuesday, February 09, 1999 3:53 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics: Automating labels > > >--------------------- >Lab-Robotics >--------------------- >Hi Matt, > >I am a Lab Manager at Myriad Genetic Laboratories, Inc. in Salt Lake >City and, since I don't get out to lab automation shows very often, I am >unaware of the companies providing solutions for putting barcodes on >microtiter plates. Could you please forward any info you might have >regarding the companies I should contact for these solutions? > >Any help you could provide would be appreciated. > >Sincerely, > >Mark Staebell > > > >RFC822 header >----------------------------------- > >RECEIVED: from SF_Database by POP_Mailbox_-1293450227 ; 10 FEB 99 18:44:29 >UT >Received: from DFW-IX4.IX.NETCOM.COM by nrgn.nrgn.com > with SMTP (QuickMail Pro Server for MacOS 1.1.1r1); 10 FEB 99 18:44:21 UT >Received: (from smap@SPAMFOIL.localhost) > by dfw-ix4.ix.netcom.com (8.8.4/8.8.4) > id RAA18013; Wed, 10 Feb 1999 17:17:37 -0600 (CST) >Message-Id: <199902102317.RAA18013@SPAMFOIL.dfw-ix4.ix.netcom.com> >Received: from nbw-nj10-40.ix.netcom.com(207.94.119.104) by >dfw-ix4.ix.netcom.com via smap (V1.3) > id rma017825; Wed Feb 10 17:16:29 1999 >From: Teresa Ferragamo >To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) >Subject: Lab-Robotics: Automating labels >Date: Wed, 10 Feb 1999 18:04:15 -0500 >MIME-Version: 1.0 >Content-Type: text/plain; > charset="iso-8859-1" >Reply-To: discussion@SPAMFOIL.lab-robotics.org >Sender: discussion@SPAMFOIL.lab-robotics.org > --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: Pepsyn@SPAMFOIL.aol.com Subject: HTS for magnetic beads Date: Tue, 16 Feb 1999 16:15:23 EST Does anyone currently work with magnetic beads, and if so, what equipment is available for their use in high throughput screening?? Any info would be helpful. Thanks. Bill Lipton, Pierce Chemical --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Matt Smicker" Subject: Re: Lab-Robotics: Automating labels Date: Tue, 16 Feb 1999 22:36:45 -0500 Jim, Do you have trouble with the labels unsticking after thawing from -80 C? It seems like most labels I've seen are only rated to -20 C. Matt Smicker -----Original Message----- From: James LaRocque To: Lab-Robotics Mailing List Date: Tuesday, February 16, 1999 10:16 PM Subject: Lab-Robotics: Automating labels >--------------------- >Lab-Robotics >--------------------- > We have been using the Zymark Presto Labeler for many months to label polypropylene plates containing samples in 100% DMSO for storage at -80 C and for shipment on dry ice. We have had no problems with the labels or the printer but we typically do not get DMSO on the labels. We currently have one labeler integrated into a Zymark XP system running through "PCS" via a DDE on the PC and a second connected directly to the system V controller. The font the printer uses only supports alpha-numeric characters but you can use excel to manipulate your list so that tabs are not a problem. If you need things like a % or # sign, you are out of luck. > >Jim LaRocque --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Charlie d'Estries" Subject: RE: Lab-Robotics: HTS for magnetic beads Date: Tue, 16 Feb 1999 23:18:20 -0500 Bill, I thought that Tecan had something that worked with magnetic beads.... But I've been wrong before! Charlie d'Estries -----Original Message----- From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org] On Behalf Of Pepsyn@SPAMFOIL.aol.com Sent: Tuesday, February 16, 1999 4:15 PM To: Lab-Robotics Mailing List Subject: Lab-Robotics: HTS for magnetic beads --------------------- Lab-Robotics --------------------- Does anyone currently work with magnetic beads, and if so, what equipment is available for their use in high throughput screening?? Any info would be helpful. Thanks. Bill Lipton, Pierce Chemical --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Raymond Reilly" Subject: Re: Lab-Robotics: HTS for magnetic beads Date: Wed, 17 Feb 1999 08:23:00 -0500 Hi Bill, CRS Automation Solutions now has a standard DNA Purification Workstation that automates all reaction steps and conditions involved in the completion of the Solid-Phase Reversible Immobilization (SPRI) procedure. It is controlled by POLARA software and includes an 8 channel bead dispenser and agitation station and two bead settling units. Please contact me for additional information and pricing. My telephone number is 908-665-7757 or email me at RFReilly@SPAMFOIL.worldnet.att.net Regards, Ray Reilly Eastern Regional Manager CRS Automation Solutions -----Original Message----- From: Pepsyn@SPAMFOIL.aol.com To: Lab-Robotics Mailing List Date: Tuesday, February 16, 1999 10:14 PM Subject: Lab-Robotics: HTS for magnetic beads >--------------------- >Lab-Robotics >--------------------- >Does anyone currently work with magnetic beads, and if so, what equipment is >available for their use in high throughput screening?? Any info would be >helpful. Thanks. >Bill Lipton, Pierce Chemical > > --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" From: Lenore Buehrer Subject: RE: Lab-Robotics: HTS for magnetic beads Date: Wed, 17 Feb 1999 08:56:09 -0500 Bill - Bio-Tek has a new model washer, the ELx405 Magna that can be used with either magnetic particle assays or standard 96-well assays. Lenore Buehrer Bio-Tek Instruments -----Original Message----- From: Pepsyn@SPAMFOIL.aol.com [mailto:Pepsyn@SPAMFOIL.aol.com] Sent: Tuesday, February 16, 1999 4:15 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: HTS for magnetic beads --------------------- Lab-Robotics --------------------- Does anyone currently work with magnetic beads, and if so, what equipment is available for their use in high throughput screening?? Any info would be helpful. Thanks. Bill Lipton, Pierce Chemical --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: "kflagg" Subject: Re: Lab-Robotics: HTS for magnetic beads Date: Wed, 17 Feb 1999 09:20:30 -0500 Bill, IGEN, International has a technology, electrochemiluminescence(ecl) which uses magnetic beads. It is a bead based technology which has a wide dynamic range, uses a nonenzymatic, non isotopic label and solution phase kinetics for rapid assay development. The instrument for HTS using ecl is currently being beta tested and very shortly ready for market. The web site is http://www.igen.com. Katherine Flagg IGEN, International -----Original Message----- From: Pepsyn@SPAMFOIL.aol.com To: Lab-Robotics Mailing List Date: Tuesday, February 16, 1999 10:04 PM Subject: Lab-Robotics: HTS for magnetic beads >--------------------- >Lab-Robotics >--------------------- >Does anyone currently work with magnetic beads, and if so, what equipment is >available for their use in high throughput screening?? Any info would be >helpful. Thanks. >Bill Lipton, Pierce Chemical > --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: Knut Skare Subject: Re: Lab-Robotics: HTS for magnetic beads Date: Wed, 17 Feb 1999 09:55:39 -0500 Hi Bill, Skatron has made dedicated bead washers in the past, and currently both the 96 well and 384 well washers have been modified to work with beads. Please contact Skatron directly if you are interested in more information. Knut Skare (800) 336-0191 Knut@SPAMFOIL.skatron.com Pepsyn@SPAMFOIL.aol.com wrote: > --------------------- > Lab-Robotics > --------------------- > Does anyone currently work with magnetic beads, and if so, what equipment is > available for their use in high throughput screening?? Any info would be > helpful. Thanks. > Bill Lipton, Pierce Chemical --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable From: "Treiber, Greg" Subject: RE: Lab-Robotics: HTS for magnetic beads Date: Wed, 17 Feb 1999 11:39:10 -0600 Hi Bill- At the recent Lab Automation '99 conference there was a poster presented by AMGEN. They are using magnetic beads in a plasmid purification protocol. The thing I thought was interesting is they did not use special equipment to perform the protocol. I've included their abstract which has an e-mail address for the author. Tracy H. Erkkila=20 Amgen, Inc.=20 David Fairbairn Kathy Henige Brian Rasnow Robert Pacifici=20 terkkila@SPAMFOIL.amgen.com Automated Plasmid DNA Minipreps =09 =09 Amgen's genomics and molecular biology programs require very large numbers of plasmid DNA mini-preparations for sequencing and mammalian cell transfections. In this paper, we describe a system which provides = a fully automated, low cost, high throughput method for the isolation of plasmid DNA from bacteria. This system utilizes an alkaline lysis = method combined with magnetic beads for separation to produce high quality DNA suitable for both sequencing and transfection. The throughput of this system is 768 plasmid DNA mini-preparations, with a yield of 9=B12mg in one eight hour shift for less than $0.75 per prep. An ORCA robot, = Zymark RapidPlate 96-channel pipettors, and a custom vacuum filtration device are the core components of the system. The system integration of these devices was accomplished with a Windows95 PC running an in-house Visual Basic-developed application that provides flexible method development, execution, and robust error recovery. =09 =09 --------------------- --------------------- -----Original Message----- From: Knut Skare [SMTP:knut@SPAMFOIL.skatron.com] Sent: Wednesday, February 17, 1999 6:56 AM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: HTS for magnetic beads > --------------------- > Lab-Robotics > --------------------- > Does anyone currently work with magnetic beads, and if so, what equipment is > available for their use in high throughput screening?? Any info would be > helpful. Thanks. > Bill Lipton, Pierce Chemical --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii From: "Susan T Ballard" Subject: Re: Lab-Robotics: HTS for magnetic beads Date: Wed, 17 Feb 1999 09:47:42 -0800 Hi. I work for BeckmanCoulter. Yes, we have solutions for magnetic beads. Contact your local BeckmanCoulter Automation Solutions Products rep for more information. Susan Ballard --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" From: "Nygaard, John" Subject: Denley WellFill 5 Date: Wed, 17 Feb 1999 16:17:26 -0800 I am in search of a used (or new) Denley WellFill 5 (8-channel) reagent dispenser. I've used them extensively in the past for various assays and appreciated their simplicity, small footprint and straightforward operation. Unfortunately Denely no longer makes them after being purchased 1-2 years ago by Labsystems. This unit specifically (NOT the Denley WellWash 5000, etc.) would be ideal for an assay I'm currently developing. I recently found one from a company who resells and services refurbished laboratory equipment. Unfortunately they have proved to be very, disappointingly, unrealiable and I am in search of another, ASAP. If anyone out there has a unit(s) they no longer have any use for and are willing to part with it, I'm ready to make a fair offer and purchase - no strings attached. Please contact me directly below if this sounds like you. Thank you! John Nygaard Assay Automation Services Manager COR Therapeutics, Inc. jnygaard@SPAMFOIL.corr.com W(650)244-6810 --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit From: "Whisenhunt, Donald W (CRD)" Subject: Used Zymark Benchmate Date: Thu, 18 Feb 1999 07:11:21 -0500 Hi, We are in the market for a used Zymark benchmate. Are there any out there? Donald Dr. Donald Whisenhunt Jr. General Electric Company Corporate Research and Development Combinatorial Chemistry Program PO Box 8 Bldg. K-1 5A24 Schenectady, NY 12301 518-387-5055 FAX 518-387-7611 Dial Comm 8*833-5055 Whisenhunt@SPAMFOIL.crd.ge.com --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" From: Lenore Buehrer Subject: RE: Lab-Robotics: plate washer Date: Thu, 18 Feb 1999 08:07:51 -0500 Jennifer and Steve - It is correct that some of the older model Bio-Tek EL403's are no longer serviced. There are several different models of EL403, though, so it is best to call Bio-Tek's Technical Assistance Center for assistance. Though Bio-Tek may not support in-house service of some of these models, we do offer assistance by phone. The EL404 microplate washers are actively supported. Contact Bio-Tek's Technical Assistance Center at 800-242-4685 or e-mail LABTAC@SPAMFOIL.biotek.com Lenore Buehrer Bio-Tek Instruments, Inc. -----Original Message----- From: Steve_Fillers@SPAMFOIL.biogen.com [mailto:Steve_Fillers@SPAMFOIL.biogen.com] Sent: Friday, February 05, 1999 9:42 AM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: plate washer --------------------- Lab-Robotics --------------------- Jennifer, I spoke with representatives from Biotek at the LabAutomation99 meeting this week. There is some confusion about this service/repair issue. Biotek is very concerned and have promised to provide clarification to this forum. Stay tuned! Steve From: "Jennifer Lee" Subject: Re: Lab-Robotics: plate washer Date: Tue, 26 Jan 1999 07:29:26 PST Hi, We have a Biotek EL403 plate washer which is broken. Because Biotek doesn't make them anymore, they no longer service them either. Does anyone know anyone who can fix them? Thanks, Jennifer Lee --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable From: Christian Deschenes Subject: Multimek instrument Date: Thu, 18 Feb 1999 08:06:16 -0800 I am trying to get a hold of a used Multimek instrument. I am ready to = buy immediately. Anyone out there looking to sell ?? Christian Christian Desch=EAnes Project Engineer Molecular BioProducts 9880 Mesa Rim Road, San Diego, CA, 92121 Phone: 619-453-7551 ext. 114 Fax: 619-452-8093 engineering@SPAMFOIL.mbpinc.com --=_X9022820412355328Y= Content-Type: multipart/alternative;boundary="------------5C7CDBB93D0B62D4F65DAA83" From: William Suits Subject: Re: Lab-Robotics: Denley WellFill 5 Date: Thu, 18 Feb 1999 16:33:07 -0500 --------------5C7CDBB93D0B62D4F65DAA83 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Try labx.com where your add is posted for free. There are some robotics items in a recent listing, though I don't remember seeing the item that you listed. Good Luck Bill Suits Chromatography Connections "Nygaard, John" wrote: > --------------------- > Lab-Robotics > --------------------- > I am in search of a used (or new) Denley WellFill 5 (8-channel) reagent > dispenser. I've used them extensively in the past for various assays and > appreciated their simplicity, small footprint and straightforward operation. > Unfortunately Denely no longer makes them after being purchased 1-2 years > ago by Labsystems. This unit specifically (NOT the Denley WellWash 5000, > etc.) would be ideal for an assay I'm currently developing. I recently found > one from a company who resells and services refurbished laboratory > equipment. Unfortunately they have proved to be very, disappointingly, > unrealiable and I am in search of another, ASAP. > > If anyone out there has a unit(s) they no longer have any use for and are > willing to part with it, I'm ready to make a fair offer and purchase - no > strings attached. Please contact me directly below if this sounds like you. > Thank you! > > John Nygaard > Assay Automation Services Manager > COR Therapeutics, Inc. > jnygaard@SPAMFOIL.corr.com > W(650)244-6810 --------------5C7CDBB93D0B62D4F65DAA83 Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit Try labx.com where your add is posted for free.  There are some robotics items in a recent listing, though I don't remember seeing the item that you listed.

Good Luck
Bill Suits
Chromatography Connections

"Nygaard, John" wrote:

---------------------
Lab-Robotics
---------------------
I am in search of a used (or new) Denley WellFill 5 (8-channel) reagent
dispenser. I've used them extensively in the past for various assays and
appreciated their simplicity, small footprint and straightforward operation.
Unfortunately Denely no longer makes them after being purchased 1-2 years
ago by Labsystems. This unit specifically (NOT the Denley WellWash 5000,
etc.) would be ideal for an assay I'm currently developing. I recently found
one from a company who resells and services refurbished laboratory
equipment. Unfortunately they have proved to be very, disappointingly,
unrealiable and I am in search of another, ASAP.

If anyone out there has a unit(s) they no longer have any use for and are
willing to part with it, I'm ready to make a fair offer and purchase - no
strings attached. Please contact me directly below if this sounds like you.
Thank you!

John Nygaard
Assay Automation Services Manager
COR Therapeutics, Inc.
jnygaard@SPAMFOIL.corr.com
W(650)244-6810

--------------5C7CDBB93D0B62D4F65DAA83-- --=_X9022820412355328Y= Content-Type: multipart/mixed; Boundary="0__=J8MfXvWmdoaLwAofy1NnhpKF1kbraO4BNrWZiJcKIJwbg6XiNSuULQy7" From: "Susan T Ballard" Subject: Re: Lab-Robotics: Multimek instrument Date: Thu, 18 Feb 1999 19:43:54 -0800 --0__=J8MfXvWmdoaLwAofy1NnhpKF1kbraO4BNrWZiJcKIJwbg6XiNSuULQy7 Content-type: text/plain; charset=us-ascii Content-Disposition: inline Christian, BeckmanCoulter does sell demo units when available. I believe your rep in San Diego is Ramone Gallo. You might want to check to see if he can locate one for you. Susan Ballard North Carolina Christian Deschenes on 02/18/99 08:06:16 AM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Mailing List) cc: (bcc: Susan T Ballard/SALES/BII) Subject: Lab-Robotics: Multimek instrument --------------------- Lab-Robotics --------------------- I am trying to get a hold of a used Multimek instrument. I am ready to buy immediately. Anyone out there looking to sell ?? Christian Christian Desch --0__=J8MfXvWmdoaLwAofy1NnhpKF1kbraO4BNrWZiJcKIJwbg6XiNSuULQy7 Content-type: text/plain; charset=iso-8859-1 Content-Disposition: inline Content-transfer-encoding: quoted-printable =EAnes Project Engineer Molecular BioProducts 9880 Mesa Rim Road, San Diego, CA, 92121 Phone: 619-453-7551 ext. 114 Fax: 619-452-8093 engineering@SPAMFOIL.mbpinc.com = --0__=J8MfXvWmdoaLwAofy1NnhpKF1kbraO4BNrWZiJcKIJwbg6XiNSuULQy7-- --=_X9022820412355328Y= Content-Type: TEXT/PLAIN From: OLEARY@SPAMFOIL.britbio.co.uk Subject: Twister interfaced to Packard Fluorocount Date: Fri, 19 Feb 1999 10:25 +0000 Does anyone have any experience with the Zymark Twister interfaced with the Packard Fluorocount/Spectracount or similar Packard kit? Our Twister has extended capacity and a barcode scanner. At the moment we are only able to generate a separate text file for the plate IDs, as identified by the barcodes. However, we urgently need them embedded in the data file from the reader. Any suggestions would be greatly appreciated. Thanks Maria O'Leary British Biotech --=_X9022820412355328Y= Content-Type: text/plain From: James LaRocque Subject: Re: Lab-Robotics: Automating labels Date: Fri, 19 Feb 1999 09:50:25 -0500 Matt, We have had no problems with the Presto labels coming off at -80C or when shipped on dry ice. We have had experience with other venders labels coming off quite easily when the plates are at -80C, but once the plates warm up they tend to stay on. Jim LaRocque ----Original Message----- From: Matt Smicker [mailto:dxs364@SPAMFOIL.psu.edu] Sent: Tuesday, February 16, 1999 10:37 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Automating labels --------------------- Lab-Robotics --------------------- Jim, Do you have trouble with the labels unsticking after thawing from -80 C? It seems like most labels I've seen are only rated to -20 C. Matt Smicker --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" From: "Smith, Todd, HMR/US" Subject: SHAKERS Date: Fri, 19 Feb 1999 09:36:49 -0600 I am looking for a shaker that has 9 or more positions capable of accepting 96 deep well plates. Spacing between the positions needs to be great enough to allow a robotic hand to pick and place the plates. The shaker must also have a reproducable origin of motion, a relatively small orbit and a high cycle time / rpm capability. Any information on vendors would be greatly appreciated. Todd Smith Biology / Chemistry Automation Selectide, a subsidiary of Hoechst Marion Roussel 1580 East Hanley Boulevard Tucson, Arizona 85737 (520) 544-5884 Phone (520) 575-1439Fax todd.smith@SPAMFOIL.hmrag.com --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: John Ringeling Subject: 384 plate reader Date: Fri, 19 Feb 1999 10:43:43 -0500 Hi all: I am wondering if people know of any good plate readers other than the Victor from Wallac that can handle 384 well plates (and optimally also does Luminescence and/or Fluorescence), but with better software than the Victor? Thanks a lot, John John Ringeling ________________________________ Ringeling@SPAMFOIL.mpi.com Millennium Pharmaceuticals Inc. Protein and Antibody Technology 40 Erie Street Cambridge, MA 02139 (617) 679 7000 X-626 Fax: (617) 374 7790 --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: gladys_range@SPAMFOIL.hgsi.com Subject: Re: Lab-Robotics: Automating labels Date: Fri, 19 Feb 1999 12:35:21 -0500 Matt, What is the procedure you use to warm the plates up.What temperature do you warm to and for how long? Is it at room temperature. Are you using polyestyrene plates?. Are the plates 384 or 96 well? What material labels , adhesive are you using? Have you used the E&K labels? I am in the process of specifying a barcode system application and it is helpful to me to learn from successfully implemented barcode systems. Thanks for all your comments. Gladys V.Range_ ______________________________________________________________________________ Subject: Lab-Robotics: Automating labels From: at INTERNET Date: 2/19/99 9:50 AM --------------------- Lab-Robotics --------------------- Matt, We have had no problems with the Presto labels coming off at -80C or when shipped on dry ice. We have had experience with other venders labels coming off quite easily when the plates are at -80C, but once the plates warm up they tend to stay on. Jim LaRocque ----Original Message----- From: Matt Smicker [mailto:dxs364@SPAMFOIL.psu.edu] Sent: Tuesday, February 16, 1999 10:37 PM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics: Automating labels --------------------- Lab-Robotics --------------------- Jim, Do you have trouble with the labels unsticking after thawing from -80 C? It seems like most labels I've seen are only rated to -20 C. Matt Smicker --=_X9022820412355328Y= Content-Type: text/plain; CHARSET=US-ASCII From: "Ramesha, Chakk {RES~Palo Alto}" Subject: RE: Lab-Robotics: 384 plate reader Date: Fri, 19 Feb 1999 10:04:10 -0800 Try Analyst from LJL. Chakk > -----Original Message----- > From: John Ringeling [SMTP:ringelin@SPAMFOIL.mpi.com] > Sent: Friday, February 19, 1999 7:44 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: 384 plate reader > > --------------------- > Lab-Robotics > --------------------- > Hi all: > > I am wondering if people know of any good plate readers other than the > Victor from Wallac that can handle 384 well plates (and optimally also > does > Luminescence and/or Fluorescence), but with better software than the > Victor? > > Thanks a lot, John > John Ringeling > ________________________________ > Ringeling@SPAMFOIL.mpi.com > > Millennium Pharmaceuticals Inc. > Protein and Antibody Technology > 40 Erie Street > Cambridge, MA 02139 > (617) 679 7000 X-626 > Fax: (617) 374 7790 --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Shannon Arrowood" Subject: Re: Lab-Robotics: 384 plate reader Date: Fri, 19 Feb 1999 14:57:40 -0800 John, If you're interested in learning more about the LJL Analyst, our web site is http://www.ljlbio.com and your local sales rep is Andree Proulx (978) 263 9882. Shannon Arrowood SouthEast & MidWest Sales Specialist Phone (919) 416-1306 http://www.ljlbio.com -----Original Message----- From: Ramesha, Chakk {RES~Palo Alto} To: Lab-Robotics Mailing List Date: Friday, February 19, 1999 10:51 AM Subject: Lab-Robotics: 384 plate reader >--------------------- >Lab-Robotics >--------------------- >Try Analyst from LJL. > >Chakk > >> -----Original Message----- >> From: John Ringeling [SMTP:ringelin@SPAMFOIL.mpi.com] >> Sent: Friday, February 19, 1999 7:44 AM >> To: discussion@SPAMFOIL.lab-robotics.org >> Subject: Lab-Robotics: 384 plate reader >> >> --------------------- >> Lab-Robotics >> --------------------- >> Hi all: >> >> I am wondering if people know of any good plate readers other than the >> Victor from Wallac that can handle 384 well plates (and optimally also >> does >> Luminescence and/or Fluorescence), but with better software than the >> Victor? >> >> Thanks a lot, John >> John Ringeling >> ________________________________ >> Ringeling@SPAMFOIL.mpi.com >> >> Millennium Pharmaceuticals Inc. >> Protein and Antibody Technology >> 40 Erie Street >> Cambridge, MA 02139 >> (617) 679 7000 X-626 >> Fax: (617) 374 7790 > --=_X9022820412355328Y= Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit From: CES1Tom@SPAMFOIL.aol.com Subject: Re: Lab-Robotics: 384 plate reader Date: Fri, 19 Feb 1999 15:34:51 EST John, The Labsystems Ascent FL has good sensitivity for both fluorescence and luminescence since it does not use fiber optics. I have had good response from customers who have purchased this unit from me. It reads petri dishes, or 12-384 well plates from top or bottom. Customers tell me the software is much easier to use than the Victor software. It can be automated with the Zymark arm, called the Assist through Labsystems. You can view details at the Labsystems site: www.labsystems.fi --=_X9022820412355328Y= Content-Type: text/plain From: "John P. Herich" Subject: RE: Lab-Robotics: 384 plate reader Date: Fri, 19 Feb 1999 12:54:29 -0800 Molecular Devices Gemini does 384 fluorescence. > -----Original Message----- > From: John Ringeling [SMTP:ringelin@SPAMFOIL.mpi.com] > Sent: Friday, February 19, 1999 9:44 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics: 384 plate reader > > --------------------- > Lab-Robotics > --------------------- > Hi all: > > I am wondering if people know of any good plate readers other than the > Victor from Wallac that can handle 384 well plates (and optimally also > does > Luminescence and/or Fluorescence), but with better software than the > Victor? > > Thanks a lot, John > John Ringeling > ________________________________ > Ringeling@SPAMFOIL.mpi.com > > Millennium Pharmaceuticals Inc. > Protein and Antibody Technology > 40 Erie Street > Cambridge, MA 02139 > (617) 679 7000 X-626 > Fax: (617) 374 7790 --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: RFTRINKA Subject: Re: Lab-Robotics: SHAKERS Date: Fri, 19 Feb 1999 18:02:00 -0800 Todd, My company, Robocon, Inc. can meet all of these requirements. Each shaker holds 6 plates. You can use 2 shakers. Email me your address and I'll send you some information. Regards, Bob Trinka, Robocon, Inc. rtrinka@SPAMFOIL.robocon.com Smith, Todd, HMR/US wrote: > > --------------------- > Lab-Robotics > --------------------- > I am looking for a shaker that has 9 or more positions capable of accepting > 96 deep well plates. Spacing between the positions needs to be great enough > to allow a robotic hand to pick and place the plates. The shaker must also > have a reproducable origin of motion, a relatively small orbit and a high > cycle time / rpm capability. Any information on vendors would be greatly > appreciated. > > Todd Smith > Biology / Chemistry Automation > Selectide, a subsidiary of Hoechst Marion Roussel > 1580 East Hanley Boulevard > Tucson, Arizona 85737 > (520) 544-5884 Phone > (520) 575-1439Fax > todd.smith@SPAMFOIL.hmrag.com --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" From: Patrick Cleveland Subject: re: Lab-Robotics: SHAKERS Date: Sat, 20 Feb 1999 09:37:05 -0800 Todd, V&P Scientific, Inc. makes a Rotary Magnetic Tumble Stirrer (VP 710A) that stirs the contents of 9 deep well microplates simultaneously using inexpensive stir discs or bars. We are able to "tumble stir" the discs or bars at speeds up to 500 RPM thus creating much greater liquid movement than conventional shakers. We recently developed the Rotary Magnetic Tumble Stirrer specially to mix the contents of deep well microplates. We have also developed simple ways for putting the stir discs or bars into the wells and also simple ways to remove them. You can see and read more about our Rotary Magnetic Tumble Stirrers on our Web catalog. http://www.vp-scientific.com/ Patrick Patrick Cleveland, Ph.D. President V&P Scientific, Inc. 9853 Pacific Heights Blvd., Suite N San Diego, CA 92121 Phone (619) 455-0643, toll free (800) 455-0644 FAX (619) 455-0703 e-mail Check out our catalog and web site at http://www.vp-scientific.com/ --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Victor Holland Subject: Automated plate unsealing Date: Tue, 23 Feb 1999 11:30:26 -0500 It seems that there are several options for automated sealing of microplates. Is there any automated system that can unseal plates? If not, how do the automated systems deal with the sealed plates? Are they taken off line for manual unsealing? Do some automated systems pierce or otherwise penetrate the seal? If so, how is the plate re-sealed? --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit From: "David W. Green" Subject: dry chemical storage Date: Tue, 23 Feb 1999 16:43:58 -0500 Since many of you that actively participate in this forum appear to come from high throughput screening and chemical compound archive groups in the pharmaceutical or agrochemical industry, I would appreciate learning how you are storing dry compounds in 4 ml vials (automated or manual retreival systems). As this part of our compound archive is growing rapidly, this is becoming more of a storage and organization issue for us. Are there alternatives to storing below room temperature in dessicated containers, and if so, have you run into chemical stability problems? I would be interested in hearing how people are doing this, especially at smaller companies. Thanks Dave Green Director, Enzymology 3-Dimensional Pharmaceuticals, Inc. 665 Stockton Drive, Suite 104 Exton, PA 19341 (610)-458-5264 x6525 FAX: (610)-458-8249 e-mail: green@SPAMFOIL.3dp.com --=_X9022820412355328Y= Content-Type: text/plain; charset="iso-8859-1" From: Patrick Cleveland Subject: re: Lab-Robotics: Automated plate unsealing Date: Tue, 23 Feb 1999 17:50:02 -0800 Unsealing microplates V&P Scientific, Inc. makes both 96 and 384 pin microplate seal piercing heads and CRS Robotics installs them on an automated seal piercing system. The pins are bevel cut and make round holes with a flap. When the bevel pin is removed the flap closes and partially seals the hole. This same partial sealing occurs after pipet tips poke through the hole. To gain a complete seal just apply another layer of sealing tape over the first. You can reach me at pcleveland@SPAMFOIL.vp-scientific.com or Ralph Wheeldon at rwheeldon@SPAMFOIL.CRSROBOTICS.com for more information. Patrick Cleveland, Ph.D. President V&P Scientific, Inc. 9853 Pacific Heights Blvd., Suite N San Diego, CA 92121 Phone (619) 455-0643, toll free (800) 455-0644 FAX (619) 455-0703 e-mail Check out our catalog and web site at http://www.vp-scientific.com/ --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: "Jennifer Nale" Subject: Used Spec Date: Fri, 26 Feb 1999 12:19:24 -0500 We are looking to buy a used vis-spectrophotometer for microplates as soon as possible. Our Molecular Devices reader is down and we need a backup. Contact me: Jennifer Nale --=_X9022820412355328Y= Content-Type: text/plain; charset="us-ascii" From: Andy Zaayenga Subject: Multidrop & DMSO Date: Fri, 26 Feb 1999 16:57:39 -0500 Is anyone using a Multidrop to dispense 100% DMSO regularly? What is your experience with life of the tubing? Do you wash the tubing after each use? How often do you use the unit, and what volumes are you dispensing? We are looking at the deepwell version. Any feedback on that model? Thanks. Andy Zaayenga TekCel Corporation Mailto:andy.zaayenga@SPAMFOIL.tekcel.com Web Site: http://tekcel.com --=_X9022820412355328Y= Content-Type: text/plain;charset="iso-8859-1" Content-Transfer-Encoding: 7bit From: "Matt Smicker" Subject: Re: Lab-Robotics: Multidrop & DMSO Date: Fri, 26 Feb 1999 21:37:36 -0500 We use a multidrop to dispense 100% DMSO regularly (daily, heavy use) to dilute compound microplates (90ul 100% DMSO). We had some trouble with tubing lifetime before we found the proper way to clean up. It is best to either empty the tubing and let it sit without rinsing overnight, or to rinse with water, then empty and let it sit overnight. At first we just rinsed with water and let the water sit in the tubing overnight. This can expand the tubing to the point where it has to be replaced (this might be due to water being picked up by DMSO which has been absorbed into the plastic tubing). I have no experience with the deepwell version of the multidrop but the standard microplate model is very reliable and low maintenance (except for changing tubing, which also requires calibration). Matt Smicker -----Original Message----- From: Andy Zaayenga To: Lab-Robotics Mailing List Date: Friday, February 26, 1999 5:19 PM Subject: Lab-Robotics: Multidrop & DMSO >--------------------- >Lab-Robotics >--------------------- >Is anyone using a Multidrop to dispense 100% DMSO regularly? >What is your experience with life of the tubing? Do you wash >the tubing after each use? How often do you use the unit, and >what volumes are you dispensing? We are looking at the deepwell >version. Any feedback on that model? Thanks. > >Andy Zaayenga >TekCel Corporation >Mailto:andy.zaayenga@SPAMFOIL.tekcel.com >Web Site: http://tekcel.com > > > --=_X9022820412355328Y= Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit --=_X9022820412355328Y=--