From: "Christopher Silva" Subject: Re: Lab-Robotics Discussion: Interface/Driver for ZymarkRobotic System Date: Tue, 04 Jan 2000 14:32:42 -0800 Ope, I assume you are using SOFTmax PRO 3.0 and have taken a look at the software commands that will support interprocess communication. They can be found in the appendix of the User Manual. I would be encouraged to hear of your success and would like to hear of any features that you may like to see in future releases of SOFTmax PRO. We plan to enhance the remote command capability to further enhance automation capabilities. Sincerely, Chris Silva Chris M. Silva Product Marketing Manager MAXline Software Molecular Devices Corp. 1311 Orleans Dr. Sunnyvale, CA 94089 USA 1-408-747-3522 1-800 635-5577 ext. 3522 ( US only) 1-408-747-3601 (fax) http://www.moleculardevices.com >>> "Ope Odusan" 12/14/99 7:23:50 AM >>> --------------------- Lab-Robotics Discussion --------------------- Hi, I have to integrate Tecan Polarion and Mol Dev Gemini Reader into Zymark Robotic systems using the PCS software. I am wondering if anybody has done this before. The idea is to use the Zymark PCS scheduler software to control the readers. I have already contacted both Tecan and Zymark. If anyone knows how or where I can get information on how to do this and can pass the info to me, I will appreciate it. Also, any vendor or individual out there that have experience doing this or have done this can also contact me. The Tecan Polarion we have uses an Excel Interface to control the reader and the Gemini has the SoftMax Pro. 3.0 software. Thanks for your help. Ope Odusan Research Scientist II HTS Robotics and Automation Wyeth-Ayerst Research Phone 914-732-3632 Email odusano@SPAMFOIL.war.wyeth.com -----oo--> Always Working On "Drugs"------oo--> --- Lab-Robotics Discussion To view statistics on this list, send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just STATS as the subject. You will receive statistics for the mailing list showing number of subscribers, domain types and messages. This is a general posting to ask the question "Where are all the EL405's gone?" Recently we've noticed a sudden drying up of the available plate washers and I was wondering if everyone else is experiencing the same situation. And more importantly does anyone know of a hidden stash of EL405 that might be available. We sure could use some EL405's. James Vincent Petersen, Ph.D. Senior Scientist Bio Automation Orchid Biocomputer Inc. 303 College Road East Princeton, NJ 08540 609.750.2282 Office 609.750.2250 Fax jpetersen@SPAMFOIL.orchidbio.com From: Jeff Cahlik Subject: RE: Lab-Robotics Discussion: Interface/Driver for ZymarkRobotic System Date: Fri, 7 Jan 2000 08:16:11 -0800 intro: Hello Chris, my name is Jeff Cahlik I have been involved in automation and integration for the past 15 years with Perkin-Elmer, Beckman Coulter and others. I am operating a Custom Automation house ' ACME-Automation' currently. Well that out of the way, I performed all the custom integration for liquid handlers with Beckman and often ran into difficulties with the various software packages. As a result of those efforts I formulated some minimum control schemes and guidline for software control. I have worked with many vendors Including MD and feel you could greatly expand your software product and thus your hardware if a module were to be written to address this. Provided you have addressed the OLE capabilities already, the module would be very manageble. I have written such an interface for the Biolage software and KC4. Your module would have great appeal to the automation community at large and would not negatively impact your standard software product offering. As you are well aware there is no real standard to work from regarding control and comm. in the automation world ( some people think they are though) . Many if not all customers are resource limited for software developement. They want to buy the 90% solution many times vs.the 100% custom solution. I would be glad to discuss this or other issues real time if you like. Lab Automation 2000? Best Regards Jeff Cahlik ACME-Automation -----Original Message----- From: Christopher Silva [SMTP:Chris_Silva@SPAMFOIL.MolDev.com] Sent: Tuesday, January 04, 2000 2:33 PM To: Lab-Robotics Discussion Mailing List Subject: Lab-Robotics Discussion: Interface/Driver for ZymarkRobotic System --------------------- Lab-Robotics Discussion --------------------- Ope, I assume you are using SOFTmax PRO 3.0 and have taken a look at the software commands that will support interprocess communication. They can be found in the appendix of the User Manual. I would be encouraged to hear of your success and would like to hear of any features that you may like to see in future releases of SOFTmax PRO. We plan to enhance the remote command capability to further enhance automation capabilities. Sincerely, Chris Silva Chris M. Silva Product Marketing Manager MAXline Software Molecular Devices Corp. 1311 Orleans Dr. Sunnyvale, CA 94089 USA 1-408-747-3522 1-800 635-5577 ext. 3522 ( US only) 1-408-747-3601 (fax) http://www.moleculardevices.com >>> "Ope Odusan" 12/14/99 7:23:50 AM >>> --------------------- Lab-Robotics Discussion --------------------- Hi, I have to integrate Tecan Polarion and Mol Dev Gemini Reader into Zymark Robotic systems using the PCS software. I am wondering if anybody has done this before. The idea is to use the Zymark PCS scheduler software to control the readers. I have already contacted both Tecan and Zymark. If anyone knows how or where I can get information on how to do this and can pass the info to me, I will ap preciate it. Also, any vendor or individual out there that have experience doing this or have done this can also contact me. The Tecan Polarion we have uses an Excel Interface to control the reader and the Gemini has the SoftMax Pro. 3.0 software. Thanks for your help. Ope Odusan Research Scientist II HTS Robotics and Automation Wyeth-Ayerst Research Phone 914-732-3632 Email odusano@SPAMFOIL.war.wyeth.com -----oo--> Always Working On "Drugs"------oo--> From: "Fred Spike, Spike International, Ltd., Wilmington, NC, USA" Subject: Charged ELISA plates Date: Mon, 10 Jan 2000 08:51:21 -0500 Does anyone know where we can find positively charged ELISA plates? Regards, Fred Fred Spike, President Spike International, Ltd PO Box 15410 Wilmington, NC 28408 USA fred@SPAMFOIL.spike.cc www.spike.cc Phone: (800) 734-9408 Toll-free Fax: (877) 561-6015 >From outside the US: Phone: (910) 790-3380 Fax: (910) 790-2023 We are looking for an alternative to the Titertek (LabSystems) MultiDrop for integration into a Sagian Core System. While the MultiDrop is an accurate, rapid, and reliable instrument for almost all bulk dispense operations, we have found that high volume dispenses into flat bottom 96 well plates (which already contain liquid) result in an unacceptable amount of splashing into adjacent wells. For this reason, we are seeking an alternative 8 to 96 channel dispenser which is already integration capable. Titertek ruled out the possibility of modifying the MultiDrop (for our specific application) but did suggest using a different product, called MAP. I would appreciate any comments on either using the MAP for our application or other alternatives. Volumes range from 35ul to 200ul; liquid is media or typical cell based assay buffers. Cheers, Dawna From: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion) Subject: Thank you for subscribing to Lab-Robotics Discussion Date: Sat, 15 Jan 2000 13:52:20 -0500 Thank you for subscribing to the Lab-Robotics Discussion mailing list. This message is sent once every 30 days to remind you of the commands which Arrow (our mailing list software) understands. When you reply to a message in a forum like this, it is generally considered "netiquette" to trim the original message down to just the portion you are replying to (if possible). Also, if you are replying to a string of messages, the same principle applies. The aim is to reduce the clutter for the other list members while preserving the sense of the message. When you post to the list, you may receive back some autoreplies from other list members email systems. This happens because some mail systems incorrectly use the "From:" heading instead of the "Reply To:" heading for autoreplies. This is unavoidable for us since we want to know who is sending a message to our list. To unsubscribe from the list, send a message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. To change to digest mode and receive messages once a day send a message with DIGEST-1 as the subject. To receive a digest once a week, send a message with a subject of DIGEST-7. To change from digest mode back to individual messages, send a message with DIGEST-OFF as the subject. For help with these and other Arrow commands, send a message to discussion@SPAMFOIL.lab-robotics.org with just HELP as the subject. Note: All Arrow commands are a single word (with no spaces) and are sent in the subject of the message to the list. Andy Zaayenga Moderator, The Lab-Robotics Discussion Mailing List mailto:andy.zaayenga@SPAMFOIL.lab-robotics.org web site: https://www.lab-robotics.org --- Age of List in Days 475 Number of Subscribers 835 Total Number of Messages Posted to List 1148 Average Number of Messages Posted Per Day 2 Dear Dawna, You may wish to consider equipment produced by Oyster Bay Pump Works for this purpose. It will be on display at Lab Automation 2000 later this month if you are attending. Otherwise check out our site or call for more info. Best regards, Patrick Gaillard Oyster Bay Pump Works, Inc. 78 Midland Avenue, PO Box 725 Hicksville, New York 11802-0725 516-933-4500 x 200 (Tel) 516- 933-4501 (Fax) prodinfo@SPAMFOIL.obpw.com www.obpw.com ----- Original Message ----- From: Dawna Houston To: Lab-Robotics Discussion Mailing List Sent: Tuesday, January 11, 2000 7:59 PM Subject: Lab-Robotics Discussion: 8-96 channel dispenser We are looking for an alternative to the Titertek (LabSystems) MultiDrop for integration into a Sagian Core System. While the MultiDrop is an accurate, rapid, and reliable instrument for almost all bulk dispense operations, we have found that high volume dispenses into flat bottom 96 well plates (which already contain liquid) result in an unacceptable amount of splashing into adjacent wells. For this reason, we are seeking an alternative 8 to 96 channel dispenser which is already integration capable. Titertek ruled out the possibility of modifying the MultiDrop (for our specific application) but did suggest using a different product, called MAP. I would appreciate any comments on either using the MAP for our application or other alternatives. Volumes range from 35ul to 200ul; liquid is media or typical cell based assay buffers. Cheers, Dawna From: "John P. Herich" Subject: RE: Lab-Robotics Discussion: 8-96 channel dispenser Date: Thu, 13 Jan 2000 17:20:00 -0800 Why not use a Sagian Multimek? > -----Original Message----- > From: Dawna Houston [SMTP:reddawn@SPAMFOIL.sprynet.com] > Sent: Tuesday, January 11, 2000 4:59 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: 8-96 channel dispenser > > We are looking for an alternative to the Titertek (LabSystems) MultiDrop > for integration into a Sagian Core System. While the MultiDrop is an > accurate, rapid, and reliable instrument for almost all bulk dispense > operations, we have found that high volume dispenses into flat bottom 96 > well plates (which already contain liquid) result in an unacceptable > amount of splashing into adjacent wells. For this reason, we are seeking > an alternative 8 to 96 channel dispenser which is already integration > capable. > > Titertek ruled out the possibility of modifying the MultiDrop (for our > specific application) but did suggest using a different product, called > MAP. I would appreciate any comments on either using the MAP for our > application or other alternatives. Volumes range from 35ul to 200ul; > liquid is media or typical cell based assay buffers. > > Cheers, > > Dawna From: TTFALCON@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: 8-96 channel dispenser Date: Thu, 13 Jan 2000 21:00:00 EST Dawna: One alternative for you would be our CyBi-Drop It is an 8 channel software-controlled dispenser module allowing extremely high precision while it runs on the fly. The Cybi-Drop features 8 or 8x2 channels and serves 96 and 384 well microplates with one reagent at a time. Its contact free and without any contamination risks. To put this in a sagian system is possible but would have coordinated with sagian. Volumes also range from .5ul to an open ended maximum range. If you would like more information please call. Troy Tremaine CyBio Inc. Southeast Regional Manager 781-376-9899 x603 From: andy.bertera@SPAMFOIL.am.apbiotech.com Subject: Re: Lab-Robotics Discussion: Charged ELISA plates Date: Fri, 14 Jan 2000 19:02:44 -0500 Fred, It is my understanding that most ELISA plates are in fact positively charged. All the ones that we use in Amersham Pharmacia Biotech's ELISA kits are. The key to getting things to stick is the coating conditions, which vary depending on the application. What is the plate being used for? Best regards, Andy. A.L.Bertera "Fred Spike, Spike International, Ltd., Wilmington, NC, USA" on 01/10/2000 08:51:21 AM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: Andy Bertera/US/xynet) Subject: Lab-Robotics Discussion: Charged ELISA plates --------------------- Lab-Robotics Discussion --------------------- Does anyone know where we can find positively charged ELISA plates? Regards, Fred Fred Spike, President Spike International, Ltd PO Box 15410 Wilmington, NC 28408 USA fred@SPAMFOIL.spike.cc www.spike.cc Phone: (800) 734-9408 Toll-free Fax: (877) 561-6015 >From outside the US: Phone: (910) 790-3380 Fax: (910) 790-2023 --- Lab-Robotics Discussion To subscribe to this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just SUBSCRIBE as the subject. Alternatively, click on this link: mailto:discussion@SPAMFOIL.lab-robotics.org?subject=subscribe From: "Pluto2" Subject: Re: Lab-Robotics Discussion: Charged ELISA plates Date: Sun, 16 Jan 2000 11:45:08 -0500 Hydrophobic interactions between proteins and ploystyrene make ELISA plates to adsorb proteins on their surfaces. I believe adding charged groups on ELISA plates will adversely affect this adsorption. As Andy mentioned we dont know your purpose of using charged groups. Pierce Chemicals sell a maleimide activated microtitre plates, where SH group containing groups can be covalently conjugated. They also sell Ni2+ chelated plates, that is designed for binding with polyhistidine containing recombinant proteins. Hope this helps. Mohan Mohan Natesan Code 6177 Naval Research Laboratory Washington DC 20375 > --------------------- > Lab-Robotics Discussion > --------------------- > Fred, > > It is my understanding that most ELISA plates are in fact positively charged. > All the ones that we use in Amersham Pharmacia Biotech's ELISA kits are. > > The key to getting things to stick is the coating conditions, which vary > depending on the application. What is the plate being used for? > > Best regards, > > Andy. > > A.L.Bertera > > > > --------------------- > Lab-Robotics Discussion > --------------------- > Does anyone know where we can find positively charged ELISA plates? > > Regards, > > Fred > > Fred Spike, President > Spike International, Ltd > PO Box 15410 > Wilmington, NC 28408 USA From: "Barbara Sullivan (Nunc)" Subject: RE: Lab-Robotics Discussion: Charged ELISA plates Date: Mon, 17 Jan 2000 13:58:40 -0500 http://nunc.nalgenunc.com/resource/bulletins/06-principles.html A quick answer: Check the Nunc brand web site product catalogue (www.nalgenunc.com Nunc Brand)for ELISA plates with specific surfaces. Read on for more info regarding the specific surfaces & what you need to choose: The adsorption of molecules to a polystyrene surface is due to different intermolecular attractive forces -- hydrophobic and hydrophilic -- depending upon the molecular nature of the molecule to be adsorbed. Unmodified polystyrene is relatively hydrophobic -- meaning there are mostly hydrophobic moieties on the surface of the well. These surfaces absorb molecules with hydrophobic moieties. "Charged" polystyrene plates is a very generic term and not often correctly used; modified would be better. Modified polystyrene plates include the mid-binding, high binding and cell culture plates, along with others. Different surfaces are made using different modification methods combined with different varieties of polystyrene. The different modification methods have been perfected to add different molecules or change molecules in order to bind specific molecules. High binding plates have both hydrophobic and hydrophilic binding sites and usually refer to high binding of immunoglobins since these plates were first characterized with respect to immunology protocols (ELISA). Cell culture plates (often even more highly charged) are a mosaic of hydrophilic and hydrophobic sites, tending to have even more hydrophilic sites than a high binding surface like a MaxiSorp surface. In regard to binding IgG, they bind well, but they also bind alot of non-specific molecules found in reagents or sample to be tested -- great for cells. "Low-binding" plates, such as PolySorp which is more hydrophobic, bind more hydrophobic molecules well (high binding for many viral antigens, lipid containing molecules, etc.) and merely bind lower amounts of Ig than "high binding" plates. The other responders are quite right in that the choice of surface is dependent on the molecule to be bound, the reagents used and the detection system employed. I suggest you check out the NNI (Nalge Nunc International) Nunc brand site. There is a tremendous amount of technical information regarding different surfaces and binding protocols. http://nunc.nalgenunc.com/resource/bulletins/06-principles.html The Nunc brand product catalogue is also there, detailing the different polystyrene, polypropylene (more hydrophobic), and specialty surfaces (covalent binding surfaces, primary amine, secondary amine, streptaviden, etc.)http://nunc.nalgenunc.com/products/index.html Most likely, the surface you need is listed there. You can reach technical support at 800 416 6862 X O, X 2531 or X 4836 for the surface experts. Alternatively, I would be happy to send anyone who is interested a technical bulletin "Principles in adsorption to Polystyrene," or the more comprehensive "Solid Phase Guide." It is all extremely useful technical information that is hard to find in one place. Check the Nunc brand web site (www.nalgenunc.com -- Nunc brand product catalogue) for the specific plates. Hope this helps. Barbara Sullivan, Ph.D. Life Science Discovery Products NNI (Nalge Nunc International) bsullivan@SPAMFOIL.nalgenunc.com -----Original Message----- From: Pluto2 [mailto:pluto2@SPAMFOIL.talarion.com] Sent: Sunday, January 16, 2000 10:45 AM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Charged ELISA plates --------------------- Lab-Robotics Discussion --------------------- Hydrophobic interactions between proteins and ploystyrene make ELISA plates to adsorb proteins on their surfaces. I believe adding charged groups on ELISA plates will adversely affect this adsorption. As Andy mentioned we dont know your purpose of using charged groups. Pierce Chemicals sell a maleimide activated microtitre plates, where SH group containing groups can be covalently conjugated. They also sell Ni2+ chelated plates,that is designed for binding with polyhistidine containing recombinant proteins. Hope this helps. Mohan Mohan Natesan Code 6177 Naval Research Laboratory Washington DC 20375 > --------------------- > Lab-Robotics Discussion > --------------------- > Fred, > > It is my understanding that most ELISA plates are in fact positively charged. > All the ones that we use in Amersham Pharmacia Biotech's ELISA kits are. > > The key to getting things to stick is the coating conditions, which vary > depending on the application. What is the plate being used for? > > Best regards, > > Andy. > > A.L.Bertera > > > > --------------------- > Lab-Robotics Discussion > --------------------- > Does anyone know where we can find positively charged ELISA plates? > > Regards, > > Fred > > Fred Spike, President > Spike International, Ltd > PO Box 15410 > Wilmington, NC 28408 USA From: BLUCK@SPAMFOIL.britbio.co.uk Subject: Lab-Robotics Discussion: High throughput solubility testing Date: Mon, 17 Jan 2000 18:55:01 +0000 Does anybody have any experience of high throughput solubility testing in a microtitre format? I'm after techniques, equipment, papers, etc. and would appreciate any help. Many thanks. Gavin Bluck Medicinal Chemist British Biotech Pharmaceuticals Ltd. Watlington Road OXFORD OX4 5LY United Kingdom t: +44 (0)1865 748747 f: +44 (0)1865 780814 e: bluck@SPAMFOIL.britbio.co.uk w: http://www.britbio.co.uk From: MJCatPAA@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: High throughput solubility testing Date: Mon, 17 Jan 2000 15:25:51 EST Dear Gavin, Process Analysis & Automation in association with Hamilton and Labsystems, offer a unique completely automated solubility test system based around the excellently engineered and very sensitive Labsystems Nephelometer and the accurate range of Hamilton pipetting stations. The control software, OVERLORD, has been supplied in the pharmaceutical industry in UK, Europe and USA, and has special facilities for Nephelometer based research and quality control experiments. Perhaps I could direct you to our web page www.paa.co.uk for further description of the services that we offer in relation to laboratory automation. You can download application notes on OVERLORD and the Nephelometer from there. We have installed many automated Nephelometer systems in the UK and Europe. Hamilton handle all the sales of these systems, a salesperson will contact you soon, however if you require further information please contact me, Malcolm Crook on 01252-373000. From: "'K' Karunaratne" Subject: Dispensing TFA Date: Mon, 17 Jan 2000 15:33:10 -0800 Hi, I'm trying to dispense TFA into a microtiter plate format, I would like to hear from anyone who has experience dispensing to multiple wells simultaneously, without the use of individual syringes, or expensive pump technology. I want to be able to adjust the dispense volume from about 1 to 4ml, and I'm trying to be consistent across the 96 wells to around 5%, my repeatability goal from shot to shot is also 5%. My absolute accuracy goal is 10%. I welcome ideas to try, but most of all sage wisdom from someone who has already tried it!!! Regards and thank you in advance K with korteks From: Stuart_Gibb@SPAMFOIL.bd.com Subject: Re: Lab-Robotics Discussion: High throughput solubility testing Date: Mon, 17 Jan 2000 21:17:14 -0500 Gavin To your point below. Please check out the BD web site at www.bd.com Look under Labware and HTS products as BD has launched a NEW CACO-2 system for doing Drug absorption testing which may help you in your work. If you have trouble getting any info please contact me directly via email. Regards Stuart Gibb Drug Discovery Manager/BD Bioscices. BLUCK@SPAMFOIL.britbio.co.uk on 01/17/2000 01:55:01 PM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: Stuart Gibb/FLKS/BDX) Subject: Lab-Robotics Discussion: High throughput solubility testing --------------------- Lab-Robotics Discussion --------------------- Does anybody have any experience of high throughput solubility testing in a microtitre format? I'm after techniques, equipment, papers, etc. and would appreciate any help. Many thanks. Gavin Bluck Medicinal Chemist British Biotech Pharmaceuticals Ltd. Watlington Road OXFORD OX4 5LY United Kingdom t: +44 (0)1865 748747 f: +44 (0)1865 780814 e: bluck@SPAMFOIL.britbio.co.uk w: http://www.britbio.co.uk --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: Peter Niggemann Subject: Laser marking of plates and such. Date: Tue, 18 Jan 2000 10:05:04 -0800 Laser marking is used quite a bit on small parts in the electronics industry. They are capable of etching a bar-code and text on just about any surface. Has anyone had any experience with laser marking of plates and such? My guess is it would work with the right kind of bar-code, small 2D like datamatrix, etc.. as the readers for these are pretty much optimized vision systems. Since we are going to be buying these 2d bar-code readers for the traqmate (sp?) tubes we are getting from Matrix I was curious as to how else we may apply the 2D bar-code technology. From: Jackbonham@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: Dispensing TFA Date: Mon, 17 Jan 2000 22:20:21 EST In a message dated 1/17/2000 8:29:55 PM Eastern Standard Time, k@SPAMFOIL.korteks.com writes: << I want to be able to adjust the dispense volume from about 1 to 4ml, >> Do you mean ul (microliter)? I'm not aware of any microplates with a 4ml capacity. Jack Bonham Titertek From: "Martin, Richard (SLH)" Subject: RE: Lab-Robotics Discussion: High throughput solubility testing Date: Tue, 18 Jan 2000 08:27:50 -0000 BMG sell a 96 well compatible nephelometer, although we have had no experience of it. Richard Martin. Celltech Chiroscience > -----Original Message----- > From: BLUCK@SPAMFOIL.britbio.co.uk [SMTP:BLUCK@SPAMFOIL.britbio.co.uk] > Sent: 17 January 2000 18:55 > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: High throughput solubility testing > > --------------------- > Lab-Robotics Discussion > --------------------- > Does anybody have any experience of high throughput solubility testing in > a > microtitre format? I'm after techniques, equipment, papers, etc. and > would > appreciate any help. > Many thanks. > > Gavin Bluck > Medicinal Chemist > British Biotech Pharmaceuticals Ltd. > Watlington Road > OXFORD > OX4 5LY > United Kingdom > > t: +44 (0)1865 748747 > f: +44 (0)1865 780814 > e: bluck@SPAMFOIL.britbio.co.uk > w: http://www.britbio.co.uk > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe The information contained in this email is intended for the personal and confidential use of the addressee only. It may also be privileged information. If you are not the intended recipient then you are hereby notified that you have received this document in error and that any review, distribution or copying of this document is strictly prohibited. If you have received this communication in error, please notify Celltech Chiroscience immediately on: +44 (0)1753 534655, or email 'is@SPAMFOIL.celltech.co.uk' Celltech Chiroscience plc 216 Bath Road, Slough, SL1 4EN, Berkshire, UK Registered Office as above. Registered in England No. 2159282 From: "Regina Gorski" Subject: Re: Lab-Robotics Discussion: Dispensing TFA Date: Tue, 18 Jan 2000 09:06:55 -0500 I'm not sure exactly what your doing and if you're using liquid handling robotics, but there's an article on small vol. pippeting on the Tomtec website: www.tomtec.com/Pages/sv.html Hope this helps ----- Original Message ----- From: 'K' Karunaratne To: Lab-Robotics Discussion Mailing List Sent: Monday, January 17, 2000 6:33 PM Subject: Lab-Robotics Discussion: Dispensing TFA > --------------------- > Lab-Robotics Discussion > --------------------- > Hi, > > I'm trying to dispense TFA into a microtiter plate format, I would like to > hear from anyone who has experience dispensing to multiple wells > simultaneously, without the use of individual syringes, or expensive pump > technology. > > I want to be able to adjust the dispense volume from about 1 to 4ml, and I'm > trying to be consistent across the 96 wells to around 5%, my repeatability > goal from shot to shot is also 5%. My absolute accuracy goal is 10%. > > I welcome ideas to try, but most of all sage wisdom from someone who has > already tried it!!! > > > Regards and thank you in advance > > > > > > K with korteks > > --- Lab-Robotics Discussion > There is a web based companion to the LRIG Discussion Mailing List > called DiscussWeb. The LRIG DiscussWeb allows for on-line messaging, > responses, and reviewing of past posts. It may be found at > https://www.lab-robotics.org/Web_Discuss/ From: BLUCK@SPAMFOIL.britbio.co.uk Subject: RE: Lab-Robotics Discussion: Dispensing TFA Date: Tue, 18 Jan 2000 14:16:37 +0000 I don't want to sound like a Luddite, but a manual multichannel pippette would be my preference. Due to TFA's corrosive and hygroscopic nature I'd keep it well away from pump heads and syringe valves. Sure, you have to pay for more man-hours but this would probably work out cheaper than the replacement parts! Gavin Bluck Medicinal Chemist British Biotech Pharmaceuticals Ltd. Watlington Road OXFORD OX4 5LY United Kingdom t: +44 (0)1865 748747 f: +44 (0)1865 780814 e: bluck@SPAMFOIL.britbio.co.uk w: http://www.britbio.co.uk -----Original Message----- From: 'K' Karunaratne [mailto:k@SPAMFOIL.korteks.com] Sent: 17 January 2000 23:33 pm To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Dispensing TFA --------------------- Lab-Robotics Discussion --------------------- Hi, I'm trying to dispense TFA into a microtiter plate format, I would like to hear from anyone who has experience dispensing to multiple wells simultaneously, without the use of individual syringes, or expensive pump technology. I want to be able to adjust the dispense volume from about 1 to 4ml, and I'm trying to be consistent across the 96 wells to around 5%, my repeatability goal from shot to shot is also 5%. My absolute accuracy goal is 10%. I welcome ideas to try, but most of all sage wisdom from someone who has already tried it!!! Regards and thank you in advance K with korteks From: Hans Monberg Subject: RE: Lab-Robotics Discussion: Charged ELISA plates Date: Tue, 18 Jan 2000 18:14:49 +0100 --------------------- Lab-Robotics Discussion --------------------- Fred, For charged ELISA plates it might be worth while to check the Exiqon web site: www.exiqon.com Best regards Hans Monberg -----Original Message----- From: Barbara Sullivan (Nunc) [mailto:BSULLIVAN@SPAMFOIL.nalgenunc.com] Sent: 17. januar 2000 19:59 To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Charged ELISA plates --------------------- Lab-Robotics Discussion --------------------- http://nunc.nalgenunc.com/resource/bulletins/06-principles.html A quick answer: Check the Nunc brand web site product catalogue (www.nalgenunc.com Nunc Brand)for ELISA plates with specific surfaces. Read on for more info regarding the specific surfaces & what you need to choose: The adsorption of molecules to a polystyrene surface is due to different intermolecular attractive forces -- hydrophobic and hydrophilic -- depending upon the molecular nature of the molecule to be adsorbed. Unmodified polystyrene is relatively hydrophobic -- meaning there are mostly hydrophobic moieties on the surface of the well. These surfaces absorb molecules with hydrophobic moieties. "Charged" polystyrene plates is a very generic term and not often correctly used; modified would be better. Modified polystyrene plates include the mid-binding, high binding and cell culture plates, along with others. Different surfaces are made using different modification methods combined with different varieties of polystyrene. The different modification methods have been perfected to add different molecules or change molecules in order to bind specific molecules. High binding plates have both hydrophobic and hydrophilic binding sites and usually refer to high binding of immunoglobins since these plates were first characterized with respect to immunology protocols (ELISA). Cell culture plates (often even more highly charged) are a mosaic of hydrophilic and hydrophobic sites, tending to have even more hydrophilic sites than a high binding surface like a MaxiSorp surface. In regard to binding IgG, they bind well, but they also bind alot of non-specific molecules found in reagents or sample to be tested -- great for cells. "Low-binding" plates, such as PolySorp which is more hydrophobic, bind more hydrophobic molecules well (high binding for many viral antigens, lipid containing molecules, etc.) and merely bind lower amounts of Ig than "high binding" plates. The other responders are quite right in that the choice of surface is dependent on the molecule to be bound, the reagents used and the detection system employed. I suggest you check out the NNI (Nalge Nunc International) Nunc brand site. There is a tremendous amount of technical information regarding different surfaces and binding protocols. http://nunc.nalgenunc.com/resource/bulletins/06-principles.html The Nunc brand product catalogue is also there, detailing the different polystyrene, polypropylene (more hydrophobic), and specialty surfaces (covalent binding surfaces, primary amine, secondary amine, streptaviden, etc.)http://nunc.nalgenunc.com/products/index.html Most likely, the surface you need is listed there. You can reach technical support at 800 416 6862 X O, X 2531 or X 4836 for the surface experts. Alternatively, I would be happy to send anyone who is interested a technical bulletin "Principles in adsorption to Polystyrene," or the more comprehensive "Solid Phase Guide." It is all extremely useful technical information that is hard to find in one place. Check the Nunc brand web site (www.nalgenunc.com -- Nunc brand product catalogue) for the specific plates. Hope this helps. Barbara Sullivan, Ph.D. Life Science Discovery Products NNI (Nalge Nunc International) bsullivan@SPAMFOIL.nalgenunc.com -----Original Message----- From: Pluto2 [mailto:pluto2@SPAMFOIL.talarion.com] Sent: Sunday, January 16, 2000 10:45 AM To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: Charged ELISA plates --------------------- Lab-Robotics Discussion --------------------- Hydrophobic interactions between proteins and ploystyrene make ELISA plates to adsorb proteins on their surfaces. I believe adding charged groups on ELISA plates will adversely affect this adsorption. As Andy mentioned we dont know your purpose of using charged groups. Pierce Chemicals sell a maleimide activated microtitre plates, where SH group containing groups can be covalently conjugated. They also sell Ni2+ chelated plates,that is designed for binding with polyhistidine containing recombinant proteins. Hope this helps. Mohan Mohan Natesan Code 6177 Naval Research Laboratory Washington DC 20375 > --------------------- > Lab-Robotics Discussion > --------------------- > Fred, > > It is my understanding that most ELISA plates are in fact positively charged. > All the ones that we use in Amersham Pharmacia Biotech's ELISA kits are. > > The key to getting things to stick is the coating conditions, which vary > depending on the application. What is the plate being used for? > > Best regards, > > Andy. > > A.L.Bertera > > > > --------------------- > Lab-Robotics Discussion > --------------------- > Does anyone know where we can find positively charged ELISA plates? > > Regards, > > Fred > > Fred Spike, President > Spike International, Ltd > PO Box 15410 > Wilmington, NC 28408 USA From: Chad Jenkins Subject: Re: Lab-Robotics Discussion: Request for info on reformatting compound library to 384 Date: Tue, 18 Jan 2000 10:45:21 -0800 Barbara, There is finally another option to seal 384 well plates. E&K Scientific now offers a Silicone Mat lid for use with 384 well plates. The mat lid is DMSO resistant, creates a tight seal with the plate and is Piercable! In addition, we now have a Silicone based adhesive for a Foil Sealing tape that withstands -80 temperatures and DMSO. The Foil adhesive Seals have not been integrated into any automated sealing instruments at this time, but will let you know once that becomes available. If you would like more information, product samples, etc... Please feel free to contact me Chad Jenkins cjenkins@SPAMFOIL.eandk.com (800) 934-8114 ext. 105 kozikowski.ba@SPAMFOIL.pg.com wrote: > --------------------- > Lab-Robotics Discussion > --------------------- > > Our screening library (solutions in 100% DMSO) is currently in 96 well format, > and we were considering reformatting our library to 384. One of our many > concerns is identifying a method to reliably seal the plates for storage. We > plan to investigate the Marsh ALPS system in conjunction with their 384 well > polypropylene v-bottom 384-well plates. A concern with this system is the > number of times that a plate can be resealed (we've heard a plate can be > resealed from 14-20x). > > Are there any other technologies/methods for forming a good DMSO-resistant seal > on a 384-well "mother" plate? Thank you in advance for any help. > > Barbara Kozikowski > Procter & Gamble Pharmaceuticals > > --- Lab-Robotics Discussion > To view statistics on this list, send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just STATS as the subject. You will receive > statistics for the mailing list showing number of subscribers, domain types and > messages. From: andy.bertera@SPAMFOIL.am.apbiotech.com Subject: Re: Plate sealing in HTS Date: Sun, 16 Jan 2000 20:59:41 -0500 Dear Discussion Group, Due to the relatively low number of plates that we have handled in the past, we have not had any need to seal plates. However, as we move towards using 1536 plates more routinely, we are now considering whether this is a requirement or not. As a consequence, I would be interested in finding out more about people's experiences with plate sealing and any help in answering the following type of questions would be welcome: 1. Is plate sealing an absolute requirement? For radioactive assays, in particular? 2. At what stage do you seal the plate? Immediately before detection? Do you ever need to remove these seals after they have been put in place? 3. Do you use 'plate tapes' for sealing or lids? What brand of seals are the best? Is heat sealing or adhesive sealing better? 4. Has anyone automated this process. If so, can you let me know how - in-house or bought-in? 5. Are any plates easier to seal than others, particular 1536-well plates ? Any help in answering these questions would be gratefully appreciated. Best regards, Andy. A.L.Bertera. From: "Thomas J. Baiga" Subject: Re: Lab-Robotics Discussion: Dispensing TFA Date: Tue, 18 Jan 2000 19:06:00 -0800 Hi K, Give me a call if you want to talk about dispensing TFA. We spent a bit of time learning the do's and don'ts of the process. We are able to dispense neat TFA with our xyz robots using the Cavro syringe pumps. However, we do some extensive mods to the liquid handling process, and you need atmosphere control for both the source and the destination. The other major problem is shielding - neat TFA will dissolve most solder points on circuit boards as well as form salt bridges that can short circuit a system. This is one of those engineering projects that ends up costing you a lot more than you thought the first time around. Maybe I can save you some time and money. If you want to come up to Carlsbad, we could discuss this process over lunch with John Kinton (Dir. of Engineering) and Steve Bush (CTO). Good luck. Tom ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ From: "Kevin Olsen" Subject: Laser Marking Date: Wed, 19 Jan 2000 07:52:42 -0500 Dear Colleagues, The compound bank at Wyeth Ayerst has been laser marking microplates since 1996. The laser marks can be decoded easily by rastering type bar code scanners. My experience has been that almost any type of bar code can be engraved into a microplate. My personal preferences are code 3 of 9 for a one-dimensional code and PDF 417 for a two-dimensional code. We have achieved our best results with microplates that have a white marking area. We burn away the white overlay and expose the underlying plastic. (Here's a tip, do not use the laser at high power. Gently remove the overlay with frequent low energy passes as opposed to a few high energy bursts.) This gives sufficient contrast for a scanner to read, but not the human eye. For this reason, we primarily use the laser marks as a back-up for the conventional label. Before investing in a laser system, I would suggest testing the marks with the scanners that are already installed in your lab. The marks were difficult to read with CCD type scanners, although recent advances in scanner technology may have overcome the difficulties that we experienced. Laser marking is well suited to automation since the laser has only three moving parts, the mirror that directs the beam (X and Y) and the ventilation system that removes the vaporized material. The marks are permanent. This is of great value to a dispensary planning to store plates at -80c for periods up to five years. I did a poster featuring this technology at ISLAR '97. Kevin Olsen Robotics and Automation Compound Bank Wyeth Ayerst Research North Middletown Road Pearl River, NY, 10965. From: Biotecinfo@SPAMFOIL.aol.com Subject: Immortalized cells for cell-based assays. Date: Wed, 19 Jan 2000 13:17:29 EST To all: I am in the process of immortalizing primary epithelial cells which will be used for HTS. The cells will be used for an assay that will be very similar to the way Caco-2 cells are used, i.e., transport studies through a monolayer grown across a cell well insert. For immortalization we have decided to use the oncogene c-myc. This gene has often been used in this capacity, but one potential side effect is that the cells lose their primary characteristics and even grow in multiple layers. My question is this...has anyone knowledge of immortalizing cells with an oncogene that is induced (simply for expanding the cells while preventing loss of the line) and then repressing the oncogene so the cell line reverts to a normal state. The idea is to grow the cells in an induced state (as immortal) and then repress the oncogene and use the cells for an cell-based assay in a normal state. Any ideas or help would be appreciated. Best regards, Dave Burden BT&C, Inc. 908-253-3444 FAX 908-575-1660 From: Donald.Bode@SPAMFOIL.aventis.com Subject: RE: Lab-Robotics Discussion: Plate sealing in HTS Date: Wed, 19 Jan 2000 14:40:23 -0500 Andy: At RPR (now Aventis), we have generally not sealed plates during HTS for at least a couple of years. Our experience with plate sealing (96 and 384, not 1536) has been with polypropylene plates containing test compounds. In that regard, we are trying to integrate a Zymark Presto sealer into our process. It uses adhesive seals on a roll (not exactly "seal tape"), which work well at room temperature but we have had problems at -20C. I think we are close to solving the problem, however. You can attach a Twister to the sealer for a capacity of up to 80 plates, and it might be possible to integrate the sealer into an Allegro system(?). We almost bought a Marsh heat sealer, but spoke to people who had experienced less than optimal results with anything other than Marsh plates so we passed. Another option is a sealer made by a German company (HJ Bioanalytik) and sold in the U.S. by Brandel (although the last time I checked, Brandel didn't know when they were actually going to be available). A plate enters from one side, is pulled through automatically, sealed with seal tape, and the tape is cut as the plate comes out the other side. It is small, works well manually (HMR, now Aventis, has used them that way in Germany), and should be readily automatable with minor modifications (which Brandel is supposed to be working on). With regard to lids, Tekcel has a great-looking system with a spring-loaded Teflon lid that seems to be automation-friendly. Chris Bode > ---------- > From: > andy.bertera@SPAMFOIL.am.apbiotech.com[SMTP:andy.bertera@SPAMFOIL.am.apbiotech.com] > Sent: Sunday, January 16, 2000 8:59 PM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: Plate sealing in HTS > > --------------------- > Lab-Robotics Discussion > --------------------- > Dear Discussion Group, > > Due to the relatively low number of plates that we have handled in the > past, we > have not had any need to seal plates. However, as we move towards using > 1536 > plates more routinely, we are now considering whether this is a > requirement or > not. As a consequence, I would be interested in finding out more about > people's > experiences with plate sealing and any help in answering the following > type of > questions would be welcome: > > 1. Is plate sealing an absolute requirement? For radioactive assays, in > particular? > 2. At what stage do you seal the plate? Immediately before detection? Do > you > ever need to remove these seals after they have been put in place? > 3. Do you use 'plate tapes' for sealing or lids? What brand of seals are > the > best? Is heat sealing or adhesive sealing better? > 4. Has anyone automated this process. If so, can you let me know how - > in-house > or bought-in? > 5. Are any plates easier to seal than others, particular 1536-well plates > ? > > Any help in answering these questions would be gratefully appreciated. > > Best regards, > > Andy. > A.L.Bertera. > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > From: Jones David Subject: RE:dispense TFA Date: Wed, 19 Jan 2000 18:12:24 -0500 It sounds as though your needs would be easily handled by our Labsystems Multidrop DW (DeepWell). The price of the Deepwell is about half of what you would expect on similar dispensers, and the accuracy and reliability of the Multidrop product line is widely known. Please call us at 1-800-522-7763. We can send you complete information and pricing. <><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><>< David W. Jones Marketing Product Manager LABSYSTEMS, North America 1-800-522-7763 david.jones@SPAMFOIL.thermobio.com > ---------- > From: 'K' Karunaratne[SMTP:k@SPAMFOIL.korteks.com] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Monday, January 17, 2000 6:33 PM > To: discussion@SPAMFOIL.lab-robotics.org > > --------------------- > Lab-Robotics Discussion > --------------------- > Hi, > > I'm trying to dispense TFA into a microtiter plate format, I would like to > hear from anyone who has experience dispensing to multiple wells > simultaneously, without the use of individual syringes, or expensive pump > technology. > > I want to be able to adjust the dispense volume from about 1 to 4ml, and > I'm > trying to be consistent across the 96 wells to around 5%, my repeatability > goal from shot to shot is also 5%. My absolute accuracy goal is 10%. > > I welcome ideas to try, but most of all sage wisdom from someone who has > already tried it!!! > > > Regards and thank you in advance > > > > > > K with korteks > > --- Lab-Robotics Discussion > To retrieve a help file on this list, send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just HELP as the subject. > > From: Ben Moshiri Subject: RE: Lab-Robotics Discussion: Dispensing TFA Date: Wed, 19 Jan 2000 20:00:21 -0600 Please take a look at Burt Goodman's article in the latest edition of JALA (Jan) - He describes how Molecumetics have been using our 48-position dispensing heads for dispensing TFA in to MiniBlocks for IRORI MiniKan cleavage. The reproducibility is around 3% and you can use a very simple bottle-top syringe pump to dispense manually. We have a 96-position version of the manifold under testing at the moment and it looks like <5% is achievable. Feel free to contact me if you are interested, or have a look at one at Lab Automation. >---------- >From: 'K' Karunaratne[SMTP:k@SPAMFOIL.korteks.com] >Reply To: discussion@SPAMFOIL.lab-robotics.org >Sent: Monday, January 17, 2000 5:33 PM >To: discussion@SPAMFOIL.lab-robotics.org >Subject: Lab-Robotics Discussion: Dispensing TFA > >--------------------- >Lab-Robotics Discussion >--------------------- >Hi, > >I'm trying to dispense TFA into a microtiter plate format, I would like to >hear from anyone who has experience dispensing to multiple wells >simultaneously, without the use of individual syringes, or expensive pump >technology. > >I want to be able to adjust the dispense volume from about 1 to 4ml, and I'm >trying to be consistent across the 96 wells to around 5%, my repeatability >goal from shot to shot is also 5%. My absolute accuracy goal is 10%. > >I welcome ideas to try, but most of all sage wisdom from someone who has >already tried it!!! > > >Regards and thank you in advance > > > > > >K with korteks > >--- Lab-Robotics Discussion >To temporarily suspend messages from this list, send an e-mail >message to discussion@SPAMFOIL.lab-robotics.org with just SUSPEND-xx (xx is a >number) as the subject. This will suspend your account for xx days >or until you send a RESUME command. > > From: "Mark Gedrych" Subject: RE: Lab-Robotics Discussion: Laser marking of plates and such. Date: Wed, 19 Jan 2000 22:32:37 -0500 Peter, The 2D bar codes that are applied to the base of microtubes are not simply laser etched onto polypropylene. Doing this would not create a mark with adequate contrast. Also, the contents of the microtube seen through the base of the tube would affect the level of contrast. Instead, the manufactures have come up with mechanisms that create a much higher contrast mark - either by printing a series of layers onto the tube where the top one is laser etched away, or by putting a doped plastic (which turns from white to black when laser marked) at the base of the tube. If you wanted to laser mark plates, then you would need the plates to have been pre-treated in a similar way. This is more a job for a plate manufacturer than a plate user. If you are going to have to apply something to a polypropylene plate yourself to allow it to be laser marked, then unless you want to print a lot of information into a very compact 2D code, or you need a very stable and permanent mark, then it seems to me that you might as well just apply a (larger) white label onto which you can ink jet print a 2D or 1D bar code or print-and-apply a 2D or 1D bar code. Mark __________________________________________________________ Mark Gedrych, The Automation Partnership Inc 266 Main Street, Suite 26, Medfield, MA 02052, USA Tel: (508) 359 5800 Ext 23 Fax: (508) 359 9352 mailto:Mark.Gedrych@SPAMFOIL.us.autoprt.com http://www.autoprt.com > -----Original Message----- > From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On > Behalf Of Peter Niggemann > Sent: Tuesday 18 January 2000 13:05 > To: Lab-Robotics Discussion Mailing List > Subject: Lab-Robotics Discussion: Laser marking of plates and such. > > > --------------------- > Lab-Robotics Discussion > --------------------- > Laser marking is used quite a bit on small parts in the electronics > industry. They are capable of etching a bar-code and text on just about > any surface. > > Has anyone had any experience with laser marking of plates and such? > > My guess is it would work with the right kind of bar-code, small 2D > like datamatrix, etc.. as the readers for these are pretty much > optimized vision systems. Since we are going to be buying these 2d > bar-code readers for the traqmate (sp?) tubes we are getting from > Matrix I was curious as to how else we may apply the 2D bar-code > technology. > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > From: "Ashton, Mark" Subject: RE: Lab-Robotics Discussion: dispense TFA Date: Thu, 20 Jan 2000 09:08:48 -0000 I have a question with regards the Labsystems Multidrop DW (DeepWell). How resistant is it to TFA and TFA vapours? Have you validated the machine with this as solvent? For it to be resistant the internal workings will need to be encased in a resistant material. What material is this? Thanks Mark Ashton ------------------------------------------- Dr. Mark R. Ashton Department Manager Oxford Asymmetry International 111 Milton Park, Abingdon, Oxon, OX14 4SD, UK Tel: + 44 (0)1235 441236/441200 Fax: + 44 (0)1235 441509 E-mail: mashton@SPAMFOIL.oai.co.uk internet: http://www.oai.co.uk/ -----Original Message----- From: Jones David [mailto:david.jones@SPAMFOIL.Thermobio.com] Sent: 19 January 2000 23:12 To: discussion@SPAMFOIL.lab-robotics.org Subject: Lab-Robotics Discussion: dispense TFA --------------------- Lab-Robotics Discussion --------------------- It sounds as though your needs would be easily handled by our Labsystems Multidrop DW (DeepWell). The price of the Deepwell is about half of what you would expect on similar dispensers, and the accuracy and reliability of the Multidrop product line is widely known. Please call us at 1-800-522-7763. We can send you complete information and pricing. <><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><>< David W. Jones Marketing Product Manager LABSYSTEMS, North America 1-800-522-7763 david.jones@SPAMFOIL.thermobio.com > ---------- > From: 'K' Karunaratne[SMTP:k@SPAMFOIL.korteks.com] > Reply To: discussion@SPAMFOIL.lab-robotics.org > Sent: Monday, January 17, 2000 6:33 PM > To: discussion@SPAMFOIL.lab-robotics.org > > --------------------- > Lab-Robotics Discussion > --------------------- > Hi, > > I'm trying to dispense TFA into a microtiter plate format, I would like to > hear from anyone who has experience dispensing to multiple wells > simultaneously, without the use of individual syringes, or expensive pump > technology. > > I want to be able to adjust the dispense volume from about 1 to 4ml, and > I'm > trying to be consistent across the 96 wells to around 5%, my repeatability > goal from shot to shot is also 5%. My absolute accuracy goal is 10%. > > I welcome ideas to try, but most of all sage wisdom from someone who has > already tried it!!! > > > Regards and thank you in advance > > > > > > K with korteks > > --- Lab-Robotics Discussion > To retrieve a help file on this list, send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just HELP as the subject. > > --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: Jackbonham@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: Dispensing TFA Date: Thu, 20 Jan 2000 11:11:44 EST Tom at Charybdis has hit a number of critical points regarding the pipetting of TFA. We had a few early "meltdowns" several years ago with TFA and have since learned how to make the Titertek MAP microplate processor behave properly. We use a single syringe to supply an eight or sixteen tip manifold for rapid dispensing in volumes greater than 25ul. Lower volumes don't do well with a manifold. It is important that the manufacturer of your pipetting equipment be aware of your desire to use TFA so they might select appropriate materials. All of our lines are pure Teflon, but even certain types of Teflon will swell with TFA. For this particular application we use a different type of Teflon in the syringe/valve assembly and we "harden" other areas of the unit such as the permanent drain. Our manifold tips are glass lined and as Tom mentioned, environmental control is helpful, as well. Although the Multidrop, mentioned earlier, is an inexpensive solution which I would like to suggest, the silicone tubing used in the peristaltic pumping unit wouldn't survive the TFA. Jack Bonham Titertek Instruments, Inc. From: Aleksandra.Weichsel@SPAMFOIL.aventis.com Subject: temperature stable deep well plates Date: Thu, 20 Jan 2000 12:34:56 -0600 Hi, I am looking for deep well plates and plate covers that withstand reaction temp 116C in DMSO. The regular PP deep well plates melt partially in these conditions. [mailto:Aleksandra.Weichsel@SPAMFOIL.aventis.com] Aleksandra Weichsel Sr. Research Chemist Selectide, Aventis Pharmaceuticals email: Aleksandra.Weichsel@SPAMFOIL.aventis.com Tel (520) 544-5833 From: Michael Sabatino Subject: 384 plate covers? Date: Thu, 20 Jan 2000 16:20:14 -0500 I am trying to help a customer find piercable covers for 384 plates. I know this was posted in the last few days but inadvertenly deleted it. I believe the company was E&K Can you repost your information. Or can someone forward that posting to me thank you Michael Sabatino LEAP Technologies. From: Stuart_Gibb@SPAMFOIL.bd.com Subject: Re: Lab-Robotics Discussion: Laser marking of plates and such. Date: Fri, 21 Jan 2000 11:34:12 -0500 Peter You may want to talk to me regarding the New Micronics 2D barcoded tubes which are being launched right now, these tubes have a proven track record of in excess of 10 years in this market. Thay have developed a unique way to manufacture the tubes with the special coding ensuring complete reprudicibilty from tube to tube not only in the dimensions of the tubes which is very important for equipment handling of the tubes but with the quality of the coding on the bottom. Micronics also have a whole system which can be handled by these major robotics companies which starts with the handling of the tubes, reading of the codes, capping of the tubes etc. Please contact me for further details directly at my vmail and I can contact you directly Vmail 1800 688 4886 x5072. BD have a booth at the Lab Autmation meeting in Palm Springs so please feel free to stop by our booth. Regards Stuart Gibb Drug Discovery Manager "Mark Gedrych" on 01/19/2000 10:32:37 PM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: Stuart Gibb/FLKS/BDX) Subject: Lab-Robotics Discussion: Laser marking of plates and such. --------------------- Lab-Robotics Discussion --------------------- Peter, The 2D bar codes that are applied to the base of microtubes are not simply laser etched onto polypropylene. Doing this would not create a mark with adequate contrast. Also, the contents of the microtube seen through the base of the tube would affect the level of contrast. Instead, the manufactures have come up with mechanisms that create a much higher contrast mark - either by printing a series of layers onto the tube where the top one is laser etched away, or by putting a doped plastic (which turns from white to black when laser marked) at the base of the tube. If you wanted to laser mark plates, then you would need the plates to have been pre-treated in a similar way. This is more a job for a plate manufacturer than a plate user. If you are going to have to apply something to a polypropylene plate yourself to allow it to be laser marked, then unless you want to print a lot of information into a very compact 2D code, or you need a very stable and permanent mark, then it seems to me that you might as well just apply a (larger) white label onto which you can ink jet print a 2D or 1D bar code or print-and-apply a 2D or 1D bar code. Mark __________________________________________________________ Mark Gedrych, The Automation Partnership Inc 266 Main Street, Suite 26, Medfield, MA 02052, USA Tel: (508) 359 5800 Ext 23 Fax: (508) 359 9352 mailto:Mark.Gedrych@SPAMFOIL.us.autoprt.com http://www.autoprt.com > -----Original Message----- > From: discussion@SPAMFOIL.lab-robotics.org [mailto:discussion@SPAMFOIL.lab-robotics.org]On > Behalf Of Peter Niggemann > Sent: Tuesday 18 January 2000 13:05 > To: Lab-Robotics Discussion Mailing List > Subject: Lab-Robotics Discussion: Laser marking of plates and such. > > > --------------------- > Lab-Robotics Discussion > --------------------- > Laser marking is used quite a bit on small parts in the electronics > industry. They are capable of etching a bar-code and text on just about > any surface. > > Has anyone had any experience with laser marking of plates and such? > > My guess is it would work with the right kind of bar-code, small 2D > like datamatrix, etc.. as the readers for these are pretty much > optimized vision systems. Since we are going to be buying these 2d > bar-code readers for the traqmate (sp?) tubes we are getting from > Matrix I was curious as to how else we may apply the 2D bar-code > technology. > > --- Lab-Robotics Discussion > To be removed from this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. > Alternatively, click on this link > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe > --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: Stuart_Gibb@SPAMFOIL.bd.com Subject: Re: Lab-Robotics Discussion: Plate sealing in HTS Date: Fri, 21 Jan 2000 12:07:06 -0500 Andy You probably want to contact Andy Zaayenga TekCel, LLC 116 South Street Hopkinton, MA 01748 Tel: (508)544-7000 ext 212 Fax: (630)604-2935 Cell: (732)672-4452 Mailto:andy.zaayenga@SPAMFOIL.tekcel.com Web Site: http://tekcel.com I believe they have developed an automated system for plate sealing. Also with regards Storage BD has a New product launch of new Polypropylene storage systems coming which I can give you some information ahead of time as we have not launched yet. If you would like to contact me and leave me a contact number on my voice mail I would be happy to send you details. Vmail 800 688 4886 x 5072 Regards Stuart Gibb Drug Discovery Manager BD Biosciences andy.bertera@SPAMFOIL.am.apbiotech.com on 01/16/2000 08:59:41 PM Please respond to discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) cc: (bcc: Stuart Gibb/FLKS/BDX) Subject: Lab-Robotics Discussion: Plate sealing in HTS --------------------- Lab-Robotics Discussion --------------------- Dear Discussion Group, Due to the relatively low number of plates that we have handled in the past, we have not had any need to seal plates. However, as we move towards using 1536 plates more routinely, we are now considering whether this is a requirement or not. As a consequence, I would be interested in finding out more about people's experiences with plate sealing and any help in answering the following type of questions would be welcome: 1. Is plate sealing an absolute requirement? For radioactive assays, in particular? 2. At what stage do you seal the plate? Immediately before detection? Do you ever need to remove these seals after they have been put in place? 3. Do you use 'plate tapes' for sealing or lids? What brand of seals are the best? Is heat sealing or adhesive sealing better? 4. Has anyone automated this process. If so, can you let me know how - in-house or bought-in? 5. Are any plates easier to seal than others, particular 1536-well plates ? Any help in answering these questions would be gratefully appreciated. Best regards, Andy. A.L.Bertera. --- Lab-Robotics Discussion To be removed from this list, please send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just UNSUBSCRIBE as the subject. Alternatively, click on this link mailto:discussion@SPAMFOIL.lab-robotics.org?subject=unsubscribe From: Peter Niggemann Subject: Re: Laser marking of plates and such. Date: Fri, 21 Jan 2000 21:45:31 -0800 At 07:32 PM 1/19/00 , Mark Gedrych wrote: Peter, The 2D bar codes that are applied to the base of microtubes are not simply laser etched onto polypropylene. Doing this would not create a mark with adequate contrast. Also, the contents of the microtube seen through the base of the tube would affect the level of contrast. Instead, the manufactures have come up with mechanisms that create a much higher contrast mark - either by printing a series of layers onto the tube where the top one is laser etched away, or by putting a doped plastic (which turns from white to black when laser marked) at the base of the tube. I am well aware of this. We have no intentions to replace what Matrix already provides. If you wanted to laser mark plates, then you would need the plates to have been pre-treated in a similar way. This is more a job for a plate manufacturer than a plate user. If you are going to have to apply something to a polypropylene plate yourself to allow it to be laser marked, then unless you want to print a lot of information into a very compact 2D code, or you need a very stable and permanent mark, then it seems to me that you might as well just apply a (larger) white label onto which you can ink jet print a 2D or 1D bar code or print-and-apply a 2D or 1D bar code. I think Kevin makes a good case for marking the plates with the laser. The marks are considerably more permanent than ink on a label. Worth exploring. From: Peter Niggemann Subject: Re: Lab-Robotics Discussion: Laser Marking Date: Fri, 21 Jan 2000 22:07:26 -0800 At 04:52 AM 1/19/00 , Kevin Olsen wrote: The compound bank at Wyeth Ayerst has been laser marking microplates since 1996. The laser marks can be decoded easily by rastering type bar code scanners. My experience has been that almost any type of bar code can be engraved into a microplate. My personal preferences are code 3 of 9 for a one-dimensional code and PDF 417 for a two-dimensional code. Kevin, Thanks for the info. It is great to know someone has had success with this technique. We have achieved our best results with microplates that have a white marking area. We burn away the white overlay and expose the underlying plastic. (Here's a tip, do not use the laser at high power. Gently remove the overlay with frequent low energy passes as opposed to a few high energy bursts.) This gives sufficient contrast for a scanner to read, but not the human eye. For this reason, we primarily use the laser marks as a back-up for the conventional label. Are these plates normally available or are they custom made? Who is the source? Before investing in a laser system, I would suggest testing the marks with the scanners that are already installed in your lab. The marks were difficult to read with CCD type scanners, although recent advances in scanner technology may have overcome the difficulties that we experienced. The cheaper CCD scanners have trouble reading even well printed barcodes so this is not surprising. In most cases we are using the Symbol LS4006 which does quite well on even bad labels. But luckily the processes we are setting up now have no barcodes yet so we have the luxury of buying whatever works well. Laser marking is well suited to automation since the laser has only three moving parts, the mirror that directs the beam (X and Y) and the ventilation system that removes the vaporized material. The marks are permanent. This is of great value to a dispensary planning to store plates at -80c for periods up to five years. Is there a big difference in lasers? There appear to be a number of types. What do you use? Also have you marked anything else? I was curious if you could mark nylon membranes as well since we would like to mark these for another process. I did a poster featuring this technology at ISLAR '97. If you have a powerpoint presentation of this I'll put it on the LRIG New England Web site. Better yet a photo of the system would go great on the robot page. :) Thanks for the info. From: Petr Kuzmic Subject: ANNC: Enzyme kinetics paper - Preprint Date: Mon, 24 Jan 2000 09:06:36 -0600 Hi, people interested in high throughput screening of enzyme inhibitors might be interested in reading a paper currently in press in Analytical Biochemistry: http://www.biokin.com/papers/AB99-0564/ The journal (don't know the volume or issue number yet) should appear in libraries a few months from now. Here is the abstract: _________________________________________________________ High-throughput screening of enzyme inhibitors: Automatic determination of tight-binding inhibition constants Petr Kuzmic 1,*, Steve Sideris 2, Lynne M. Cregar 2, Kyle C. Elrod 2, Kenneth D. Rice 3, and James W. Janc 2 1 BioKin, Ltd., P.O. Box 8336, Madison, WI 53708 2 Department of Enzymology and 3 Department of Medicinal Chemistry Axys Pharmaceuticals, Inc., 180 Kimball Way, South San Francisco, CA 94080 ABSTRACT Determination of tight-binding inhibition constants by nonlinear least-squares regression requires sufficiently good initial estimates of the best-fit values. Normally an initial estimate of the inhibition constant must be provided by the investigator. This paper describes an automatic procedure for the estimation of tight-binding inhibition constants directly from dose-response data. Because the procedure does not require human intervention, it was incorporated into an algorithm for high-throughput screening of enzyme inhibitors. A suitable computer program is available electronically (http://www.biokin.com). Representative experimental data are shown for the inhibition of human mast-cell tryptase. _____________________________________________________________________ Petr Kuzmic, Ph.D. * BioKin, Ltd. * Consulting & Software Development http://www.biokin.com * (608) 256-4790 * (608) 256-1269 FAX From: Chad Jenkins Subject: Re: Lab-Robotics Discussion: Request for info on reformatting compound library to 384 Date: Mon, 24 Jan 2000 10:03:04 -0800 --------------------- Lab-Robotics Discussion --------------------- Barbara, There is finally another option to seal 384 well plates. E&K Scientific now offers a Silicone Mat lid for use with 384 well plates. The mat lid is DMSO resistant, creates a tight seal with the plate and is Piercable! In addition, we now have a Silicone based adhesive for a Foil Sealing tape that withstands -80 temperatures and DMSO. The Foil adhesive Seals have not been integrated into any automated sealing instruments at this time, but will let you know once that becomes available. If you would like more information, product samples, etc... Please feel free to contact me Chad Jenkins cjenkins@SPAMFOIL.eandk.com (800) 934-8114 ext. 105 kozikowski.ba@SPAMFOIL.pg.com wrote: --------------------- > Lab-Robotics Discussion > --------------------- > > Our screening library (solutions in 100% DMSO) is currently in 96 well format, > and we were considering reformatting our library to 384. One of our many > concerns is identifying a method to reliably seal the plates for storage. We > plan to investigate the Marsh ALPS system in conjunction with their 384 well > polypropylene v-bottom 384-well plates. A concern with this system is the > number of times that a plate can be resealed (we've heard a plate can be > resealed from 14-20x). > > Are there any other technologies/methods for forming a good DMSO-resistant seal > on a 384-well "mother" plate? Thank you in advance for any help. > > Barbara Kozikowski > Procter & Gamble Pharmaceuticals > From: Lyne Larose Subject: 96/384 well plate readers Date: Thu, 23 Dec 1999 03:16:57 -0500 Question to group, Can anyone recommend a good 96/384 well fluorescence and/or absorption plate reader that can transfer reading values directly into the software of a liquid handling robot such as the Packard MultiProbe II ? Many Thanks, Scott Kelso Agriculture & Agri-Food Canada 960 Carling Avenue Ottawa, Ontario. K1A 0C6 (613)759-1657 kelsos@SPAMFOIL.em.agr.ca From: MJCatPAA@SPAMFOIL.aol.com Subject: Re: Lab-Robotics Discussion: 96/384 well plate readers Date: Wed, 26 Jan 2000 02:31:14 EST Dear Scott, We offer a fully integrated system based on out own software package (OVERLORD), the Hamilton pipetting robots and LabSystems plate readers. Please send more details about your method and sample throughput so we can give you a better idea of which equipent would be most suitable. Regards Malcolm Crook Process Analysis & Automation Falcon House Fernhill Road Farnborough GU14 9RX UK Phone: 44 1252 373000 FAX: 44 1252 371922 From: "fabrice turlais" Subject: Re: Lab-Robotics Discussion: 96/384 well plate readers Date: Wed, 26 Jan 2000 01:22:03 PST HI Scott, why don't you try the Wallac Victor 2? We have using it for about a year, it works nicely and can save files as excel spreadsheet. You can then get the multiprobe II to read the file. Fabrice Turlais CRC CT, Institute of Cancer Research Sutton SM2 5NG, UK. From: Lyne Larose Reply-To: discussion@SPAMFOIL.lab-robotics.org To: discussion@SPAMFOIL.lab-robotics.org (Lab-Robotics Discussion Mailing List) Subject: Lab-Robotics Discussion: 96/384 well plate readers Date: Thu, 23 Dec 1999 03:16:57 -0500 --------------------- Lab-Robotics Discussion --------------------- Question to group, Can anyone recommend a good 96/384 well fluorescence and/or absorption plate reader that can transfer reading values directly into the software of a liquid handling robot such as the Packard MultiProbe II ? Many Thanks, Scott Kelso Agriculture & Agri-Food Canada 960 Carling Avenue Ottawa, Ontario. K1A 0C6 (613)759-1657 kelsos@SPAMFOIL.em.agr.ca --- Lab-Robotics Discussion To review archived messages from this list, send an e-mail message to discussion@SPAMFOIL.lab-robotics.org with just RESEND-xx (xx is a number) as the subject. You will get a single digest message for the last xx days of messages. ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com From: "Ramesha, Chakk {High~Palo Alto}" Subject: RE: Lab-Robotics Discussion: 96/384 well plate readers Date: Wed, 26 Jan 2000 12:52:41 -0800 Try victor from Wallac. Chakk > -----Original Message----- > From: Lyne Larose [SMTP:pharoah@SPAMFOIL.sympatico.ca] > Sent: Thursday, December 23, 1999 12:17 AM > To: discussion@SPAMFOIL.lab-robotics.org > Subject: Lab-Robotics Discussion: 96/384 well plate readers > > --------------------- > Lab-Robotics Discussion > --------------------- > Question to group, > > Can anyone recommend a good 96/384 well fluorescence and/or absorption > plate reader that can transfer reading values directly into the software > of a liquid handling robot such as the Packard MultiProbe II ? > > Many Thanks, > > Scott Kelso > Agriculture & Agri-Food Canada > 960 Carling Avenue > Ottawa, Ontario. > K1A 0C6 > (613)759-1657 > kelsos@SPAMFOIL.em.agr.ca > > --- Lab-Robotics Discussion > To subscribe to this list, please send an e-mail message to > discussion@SPAMFOIL.lab-robotics.org with just SUBSCRIBE as the subject. > Alternatively, click on this link: > mailto:discussion@SPAMFOIL.lab-robotics.org?subject=subscribe From: "Feiglin, Marc" Subject: RE: Lab-Robotics Discussion: Request for info on reformatting compound library to 384 Date: Wed, 26 Jan 2000 21:47:27 -0500 Ask Corning about their RoboLid. This is a lid that combines the sealing capability of a silicone mat, and the handling characteristics of a polystyrene lid. We use these lids for our sample collection. The lids can easily be remove and replaced by automation. > --------------------- > > Lab-Robotics Discussion > > --------------------- > > > > Our screening library (solutions in 100% DMSO) is currently in 96 well > format > , > > and we were considering reformatting our library to 384. One of our > many > > concerns is identifying a method to reliably seal the plates for > storage. We > > > plan to investigate the Marsh ALPS system in conjunction with their 384 > well > > polypropylene v-bottom 384-well plates. A concern with this system is > the > > number of times that a plate can be resealed (we've heard a plate can > be > > resealed from 14-20x). > > > > Are there any other technologies/methods for forming a good > DMSO-resistant se > al > > on a 384-well "mother" plate? Thank you in advance for any help. > > > > Barbara Kozikowski > > Procter & Gamble Pharmaceuticals > > > > --- Lab-Robotics Discussion > There is a web based companion to the LRIG Discussion Mailing List > called DiscussWeb. The LRIG DiscussWeb allows for on-line messaging, > responses, and reviewing of past posts. It may be found at > https://www.lab-robotics.org/Web_Discuss/ > From: Paul Skerker Subject: pin tools for compound handling Date: Thu, 27 Jan 2000 13:42:16 -0500 Can anyone share their experience with using pin tools for compound plating. We would like to add 50-250 nl of compound in 100% dmso to dry plates. Any thoughts or issues ?? Thanks, Paul Skerker ______________________________ Paul Skerker, Ph.D. Leukosite, Inc. 215 First Street Cambridge, MA 02142 paul_skerker@SPAMFOIL.leukosite.com (617) 551-3692 From: Todd.Smith@SPAMFOIL.aventis.com Subject: Mini_Pumps Date: Fri, 28 Jan 2000 13:02:24 -0600 I am looking for a minature 24V pump in either a gear type or a diaphram type configuration. I need all the wetted parts to be teflon or PP. If anyone has experience with these types of mini pumps I would like to talk with you. Todd Smith Biology / Chemistry Automation Selectide, a subsidiary of Aventis 1580 East Hanley Boulevard Tucson, Arizona 85737 (520) 544-5884 Phone (520) 575-1439 Fax todd.smith@SPAMFOIL.aventis.com From: "David Allen" Subject: RE: Lab-Robotics Discussion: Mini_Pumps Date: Fri, 28 Jan 2000 19:00:39 -0800 > I am looking for a minature 24V pump in either a gear type or a diaphram > type configuration. I need all the wetted parts to be teflon or PP. If > anyone has experience with these types of mini pumps I would like to talk > with you. > > Todd Smith FMI and Cole Parmer have low voltage, DC, gear pumps. I've worked with CP's peristaltic pumps and FMI's low voltage piston pumps. both are highly reliable and easy to interface. if it _must_ be teflon or PP, you may have to go with a diaphragm pump. I don't remember seeing plastic gear pumps. what kind of flow rates and pressures do you need? does it have to be DC? da From: "PABLO CORREA" Subject: AutoRobot Software sellers Date: Sat, 29 Jan 2000 05:42:52 PST I'm looking for the current seller of the program AutoRobot, formerly designed and sold by Rainbow Design, used to simulate robotic cells with AutoCAD environment. Otherwise, do you know another similar software? My address: pcm_1@SPAMFOIL.hotmail.com. Regards, Pablo Correa Electro-Mechanical Engineer Robotics Researcher From: David Coombs Subject: Re: Lab-Robotics Discussion: Mini_Pumps Date: Sat, 29 Jan 00 19:59:40 -0400 Hello, I use a 12V version of a gear pump made by Greylor in Florida. I don't know if they make a 24V version, but you can ask. The pump is powerful enough, but the motor is very noisy electronically and needs extensive suppression and isolation to keep it from interfering with the computer in my instrument. david Pump: PQ-12DC po num. part no. 120000 Address: Cape Coral Fl phone: 941-574-2011 Dr. David Coombs BioComp Instruments, Inc. 650 Churchill Row Fredericton, NB Canada E3B 1P6 Phone: 800-561-4221 Fax: 506-453-3583 Email: dhc@SPAMFOIL.unb.ca Web page: http://131.202.97.21/ From: "Thomas J. Baiga" Subject: Re: Lab-Robotics Discussion: Mini_Pumps Date: Sun, 30 Jan 2000 10:41:42 -0800 At 01:02 PM 1/28/00 -0600, you wrote: --------------------- Lab-Robotics Discussion --------------------- I am looking for a minature 24V pump in either a gear type or a diaphram type configuration. I need all the wetted parts to be teflon or PP. If anyone has experience with these types of mini pumps I would like to talk with you. Try looking at these pumps. Their application people are also quite helpful. For some first-hand knowledge from engineers using these pumps for organic/inorganic chemistry applications, give Charybdis a call and ask to speak with John Kinton. Micropump Inc. 1402 NE 136th Avenue Vancouver, WA 98684-0818 Office Phone: (360) 253-2008 Voice Mail: (360) 253 3220 General Facsimile: (360) 253 8249 Sales Facsimile: (800) 222 9565 (USA only) Email: info@SPAMFOIL.micropump.com URL: www.micropump.com ----------------------------------------------------------------------- Thomas J. Baiga President and Chief Executive Officer Charybdis Technologies, Inc. 5925 Priestly Drive Suite 101 Carlsbad, California 92008 Phone: 760.930.6100 Fax: 760.930.6099 tjbaiga@SPAMFOIL.charybtech.com http://www.charybtech.com/ From: "David Allen" Subject: RE: Lab-Robotics Discussion: AutoRobot Software sellers Date: Sun, 30 Jan 2000 22:55:45 -0500 > I'm looking for the current seller of the program AutoRobot, formerly > designed and sold by Rainbow Design, used to simulate robotic > cells with > AutoCAD environment. > > Otherwise, do you know another similar software? > I've been using Simul8 with success. I used it to model a commercial robotics system and it showed ahead of time that their implementation would not meet spec. when the trials were run, the actual values were within 10% of the predicted values. it also showed that certain changes would have brought it into spec. those changes were much easier to make and try on the computer than at the factory. it's fairly easy to setup and they have great tech support. at $495, it's an excellent value. the graphics aren't as 'pretty' as AutoCAD but.... da From: "Mark Orlowski" Subject: RNase free tips Date: Mon, 31 Jan 2000 10:57:23 -0500 I am using a Biomek2000 and Quadra96 for an assay in which all reagents and tips must be RNase free. Beckman and Quadra have both told me they do not sell autoclaved/PCR-ready tips for thier instuments. If I autoclave them myself TomTec says the Quadra tips will melt, and Beckman says they might deform enough so that the robot cannot pick them up. Can anyone offer suggestions or know of alternate vendors who sell these tips? Thanks. M. Orlowski From: Gladys_Range@SPAMFOIL.hgsi.com Subject: Re: Lab-Robotics Discussion: Mini_Pumps Date: Mon, 31 Jan 2000 11:32:09 -0500 You might want to explore these sources. I have used Teflon pumps from all of them and was very happy with their performance. ASF, Inc (Diaphragm and gear pumps) Siggy Mueller 2100 Narcross Parkway #120 Narcross Georgia (770)441-3611 The Lee Company (Mini pumps) 2 Pettipaug Rd Westbrook Ct 06498-0424 (203) 399-6281 Pall Corporation 25 Harbor Park Drive Port Washington, NY 11050 (516) 484-3600 G.V. Range (301)610-5790 gladys_range@SPAMFOIL.hgsi.com From: Juan_Antonio_Mostacero@SPAMFOIL.sbphrd.com Subject: Robotic Centrifuges Date: Mon, 31 Jan 2000 18:02:30 +0100 Does anybody knows if there is any microplate centrifuge that could be integrated in a robotic system like Sagian Core System or similar? Thanks, Juan From: David Subject: Re: Lab-Robotics Discussion: AutoRobot Software sellers Date: Mon, 31 Jan 2000 17:22:32 +0000 PABLO CORREA wrote: > > --------------------- > Lab-Robotics Discussion > --------------------- > I'm looking for the current seller of the program AutoRobot, formerly > designed and sold by Rainbow Design, used to simulate robotic cells with > AutoCAD environment. > > Otherwise, do you know another similar software? > > My address: pcm_1@SPAMFOIL.hotmail.com. > > Regards, > > Pablo Correa > Electro-Mechanical Engineer > Robotics Researcher Try Workspace 5 from Flow Software Technologies, email Simon Tilley on stilley@SPAMFOIL.workspace5.com David -- ________________________________________________________ David N. Sands, ST Robotics International Website: http://www.strobotics.com The name is Baud, James Baud. ASCII stupid question, get a stupid ANSI! Enter any 11-digit prime number to continue... From: "Richard Rodriguez" Subject: Bohdan Label Automator Date: Mon, 31 Jan 2000 12:43:26 -0800 Would anyone care to share your experiences with the Bohdan Label Automator both pro and con? I may be contacted at rrodriguez@SPAMFOIL.panlabs.com. Richard Rodriguez From: "Lance A. Larka" Subject: Re: Lab-Robotics Discussion: RNase free tips Date: Mon, 31 Jan 2000 22:08:37 -0800 You might want to try contacting Axygen Scientific. I know that they make Biomek compatible tip racks and I know that they make gamma-sterilized plasticware as well. They are a manufacturer only, but I'm sure will point you to a distributor. Their number is 510-494-8900 and the web URL is www.axygen.com. My personal experience with the Beckman tips is that they are deformed enough to begin with and autoclaving them only makes them worse. Many an assay of mine has aborted because the MP8 head lifted the tip rack along with the tips. Good luck. -Lance ************************************************** Lance A. Larka Research Scientist Operon Technologies, Inc. 1000 Atlantic Ave. Suite 108 Alameda, CA 94501 Ph. 510.865.8644 ex. 152 vm. 224 Fx. 561.828.8327 E-mail: llarka@SPAMFOIL.operon.com HTTP://www.operon.com/ "Science answers the question you asked, Not the question you thought you asked." ************************************************** Mark Orlowski wrote: > --------------------- > Lab-Robotics Discussion > --------------------- > I am using a Biomek2000 and Quadra96 for an assay in which all reagents > and tips must be RNase free. Beckman and Quadra have both told me they do > not sell autoclaved/PCR-ready tips for thier instuments. If I autoclave > them myself TomTec says the Quadra tips will melt, and Beckman says they > might deform enough so that the robot cannot pick them up. Can anyone > offer suggestions or know of alternate vendors who sell these tips? > Thanks. > > M. Orlowski >