Notes
Slide Show
Outline
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3456-well cAMP Assay
  • Michael Weber
  • Department of Automated Biotechnology
  • Merck
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Principles of Assay Technology
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Biology of a Gi-coupled Receptor
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3456-well Assay Protocol
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Forskolin Max, Min & Cell Titration
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Stimulation Optimization
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Forskolin and Antagonist Dose Response:
in 384- & 3456-well format
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Comparison of Alternative cAMP Technologies:
Lance vs HTRF vs DiscoveRx EFC
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Viewlux Image of Multi Compound Dose Response
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DMSO Sample Plate
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Sample Compound Plate
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Run Set Chart
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Conclusion
  • The 3456 –well EFC HitHunter cAMP assay exhibited comparable assay sensitivity and robustness to a 384-well plate format assay.
  • Miniaturization of the EFC assay into 3456-well format for a uHTS resulted in significant cost savings
    • 6-fold reduction in cell-culture associated costs
    • 25-fold reduction in reagent costs
  • 3456-well DiscoveRx cAMP assays are amenable to fully automated robotic uHTS
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Acknowledgements
  • Priya Kunapuli
  • Rui Tang
  • Tung Fong
  • Oleg Kornienko
  • Delphine Collin
  • Berta Strulovici