1	SHOW – Sample Handling Operation Wizard Ping Du 4 Fullerton Place Livingston, NJ 07039 Land: 973-422-1919 Cell: 858-603-5043
2	Abstract Manual sample handling, such as moving samples or recording data visually, is a tedious and error prone process. Sample Handling Operation Wizard (SHOW) is a system developed to guide manual sample handling in laboratories. It comprises a sample tray mounted on a 15” flat - panel computer monitor. Up to four transparent micro titer plates and up to 8 reagent vials may be placed on the sample tray. Images of the wells of the plates and vials are generated on the monitor and controlled by computer software.
3	Abstract (continued) These images are directly aligned with the positions of the physical wells or vials. By highlighting the wells or vials involved in a sample handling step of a predefined protocol, manual operations can be performed with precise guidance from the system. As a result, the risk of locating a wrong sample or placing a sample at a wrong location can be minimized, and sample handling operations become more efficient and less stressful.
4	Introduction Manual sampling handling Tedious Error-prone Stressful Lack of tracking Automated liquid handlers Complex High maintenance cost Not portable
5	Objective Display graphic guidance underneath each sample and text memo in close vicinity for manual sample handling Handle samples without memorizing or reading instructions of a protocol Define and store sample handling protocols Modify and test sample handling protocols Facilitate automatic recording sample handling details, such as amount transferred and the time of transfer
6	Design Principle Simple and easy to operate Inexpensive to build Portable and flexible Hardware A computer with 15” flat-panel monitor A sample tray holding four plates and 8 vials Software Define protocol Test protocol Execute protocol
7	Hardware
8	Software Flowchart
9
10	Software GUI
11	Control Menu – Home, Settings
12	Control Menu – Protocol, External
13	Control Menu – Tasks, Steps
14	Demo Application 1 - Parallel Synthesis Protocol: 4 x 5 library Task 1 Reagent A dissolution into vials Reagent A transfer from vials to 24-well synthetic plate Task 2 Reagent B dissolution into vials Reagent B transfer from vials to 24-well synthetic plate
15	Demo Application 2 Compound Plating Protocol: Compound Plating Task 1 Transfer from 24-well synthetic plate to 48- well storage plate Transfer from 24-well synthetic plate to 96- well QC plate Transfer from 24-well synthetic plate to 384-well assay plate
16	Demo Application 3 Serial Dilution Protocol: Serial Dilution Task 1 Transfer from stock solution to Row A of a 96-well assay plate Split samples to Rows B to H with two-fold decreasing volumes Add solvent
17	Potential Application - Data Recording Protocol: Record MIC (minimal inhibition concentration for bacterial growth) Task 1 Load a serial dilution assay plate Visually inspect cell growth Record MIC by clicking on the well with the lowest concentration and no cell growth (under implementation)
18	Conclusion The SHOW system provides guidance to manual sample handling Flexible design allows different plate formats System is simple and inexpensive Manual sample handling becomes more accurate and less stressful
19	"Acknowledgement:" Acknowledgement: Dr. Hanghui Liu Contact: Dr. Ping Du 4 Fullerton Place Livingston, NJ 07039 Land: 973-422-1919 Cell: 858-603-5043