Notes
Slide Show
Outline
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Shared Materials Repositories
at the Harvard Institute of Proteomics
  • DF/HCC DNA Resource Core
  • Protein Structure Initiative Materials Repository (PSI-MR)
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Protein Function
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Protein Function
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High-Throughput Protein Studies
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Automated Colony Picking
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Plating Bacteria
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Automated Colony Picking
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FLEXGene Clone Production
  • Hundreds of clones per week
  • Quality control measures
    • Single-colony selection
    • Phage-resistant host strains
    • Barcode label tracking
  • Fully tracked in the FLEXGene LIMS
  • Routine protocols for transfer to expression vectors
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FLEX gene ORF collections
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Storage Sample Production
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Sample Storage Production
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Sample Storage Production
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Sample Storage Production
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Sample Storage—BioBank Automated Freezer Storage System
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Connect samples to biological information about clones?
  • Plasmid Information Database (PlasmID)
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PlasmID
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Organization Facilitates Searches
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Shared Repositories at HIP
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Clone Request Fulfillment
  • Minimally restrictive MTAs
  • Pre-approval agreements for MTAs
  • Accommodate special MTAs, restrictions
  • On-line request via PlasmID
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Clone Request Fulfillment
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FLEXGene Clones
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